Alex Moloney

a cartoon of spongebob holding a piece of paper with memecenter.com written on the bottom ALT: a cartoon of spongebob holding a piece of paper with memecenter.com written on the bottom

Running 2 hours over time, first session of the day..
"Could we keep it to one question each from the audience"
The "question" from the audience...

#handsoff My niece in Boston.

My colleague @amoloney.bsky.social is hiring an intern for the summer in Bristol! Alex is a great person to collaborate with and I'm sure whoever works with him will have a blast this summer. biotechne.wd5.myworkdayjobs.com/en-US/Biotec...

Thanks Stephanie!

At his last celebration, he said something that stayed with me: "When you start, you think science is about the papers...then you realize it's about the people."

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS🍬🍬🍬

For the first time, we are able to image the molecular architecture of the glycocalyx at Ångström resolution. A dream come true!

Read the preprint: biorxiv.org/content/10.110…

And find our tweetorial below!
#glycotime

Your wish is my command. Licensed by Tocris and coming imminently!

Live imaging of the extracellular matrix with a glycan-binding fluorophore - Nature Methods Rhobo6 is a cell-impermeable small-molecule fluorophore that displays reversible fluorogenic binding to glycans, making it a general, wash-free, and non-perturbative label for the extracellular matrix...

Check it out, powerful new method for optical imaging of the live ECM, from @hhmijanelia.bsky.social Group Leader Kayvon Pedram #proudofalumni #glycotime

www.nature.com/articles/s41...

Graphic showing modification of mRNA with ADP-ribose mediated by bacterial enzyme cmdTAC

Check it out! Another novel RNA modification - ADP-ribosylation - that was previously only known on proteins. A cousin to #glycoRNA 👏

www.nature.com/articles/s41...

And collaborators! 😄

Ow boy! Just when I thought I couldn't be impressed by a JQ1 containing compound anymore... it gets mashed with my favourite Janelia Fluor dye (JF635) and a halotag ligand. Exceptional work #chembio

Exceptional degrader ("targeted glue") development story. Tour de force 👏

Controversial COVID study that promoted unproven treatment retracted after four-year saga Paper on hydroxychloroquine led by French researcher Didier Raoult is second-most-cited study ever to be withdrawn.

Controversial COVID study that promoted unproven treatment retracted after four-year saga

Paper on hydroxychloroquine led by French researcher Didier Raoult is second-most-cited study ever to be withdrawn.

@richvn.bsky.social reports at Nature.

www.nature.com/articles/d41...

Tough one! For me:
2000 Bertozzi Science Staudinger paper.
2008 Fox/Devaraj Tetrazine papers
2012 Chin/Fox JACS UAA incorporation.

Lipid-directed covalent fluorescent labeling of plasma membranes for long-term imaging, barcoding and manipulation of cells Fluorescent probes for cell plasma membrane (PM) are generally based on amphiphilic anchors that incorporate non-covalently into biomembranes. Therefore, they are not compatible with fixation and permeabilization, presence of serum, or cell co-culture because of their exchange with the medium. Here, we report a concept of lipid-directed covalent labeling of PM, which exploits transient binding to lipid membrane surface generating high local dye concentration, thus favoring covalent ligation to random proximal membrane proteins. This concept yielded a class of fluorescent probes for PM (MemGraft), where a cyanine dye (Cy3 and Cy5) bears at its two ends low-affinity membrane anchor and reactive group: an activated ester or a maleimide. We found that MemGraft probes with these reactive groups provide efficient PM labelling, in contrast to a series of control compounds, including commercial Cy3-based labels of amino and thiol groups, revealing the crucial role of the membrane anchor combined with high reactivity of activated ester and a maleimide groups. In contrast to conventional PM probes, based on non-covalent interactions, MemGraft labelling approach is compatible with cell fixation, permeabilization, trypsinization and presence of serum. The latter allows long-term cell tracking and video imaging of cell PM dynamics without signs of phototoxicity. The covalent strategy also enables staining and long-term tracking of co-cultured cells labelled in different colors without probes exchange. Moreover, combination of different ratios of MemGraft-Cy3 and MemGraft-Cy5 probes enabled long-term cell barcoding in at least 5 color codes, important for tracking and visualizing multiple cells populations. Ultimately, we found that MemGraft strategy enables efficient biotinylation of cell surface, opening the path to cell surface engineering and cell manipulation. ### Competing Interest Statement The authors have declared no competing interest.

Interesting ChemRxiv preprint by the group of Andrey Klymchenko. They develop fluorescent probes that enrich in the membrane and then covalently label proteins. This leads to plasma membrane labeling, which is resistant to cell permeabilization. #ChemBio www.biorxiv.org/cont...

Amazing! Thanks Chris

Really hope journals start to integrate bluesky 🦋 into altmetric soon! Any plans? @acspublications.bsky.social @cellpress.bsky.social

Are you up on organ clocks?
—Today @washingtonpost.com www.washingtonpost.com/wellness/202...
—Previously on Ground Truths
erictopol.substack.com/p/the-emerge...

Me whizzing to the structures in this preprint 👀.

Align on vision, differ on skill sets. Love this idea

Eureka Moments Shared by Chemists. Hints at Enhancing One’s Own Creativity (and Even One’s Joy) Interviews were conducted with 18 chemists from several subdisciplines of chemistry and include a diversity of demographics on the topic of creativity as seen through the eyes of Eureka moments. The experiences fell within three categories, i.e., (1) analytical problem-solving which can be reconstructed into a series of logical steps that can be identified; (2) memory retrieval processes of previously acquired knowledge; and (3) insights characterized by a sudden and unexpected understanding. There were variations of detail within each category. Suggestions for enhancing the probability of experiencing Eureka moments are provided.

A really fun piece in ACS Central Science that takes a deep dive into Eureka moments in chemical discovery! 💡

"Eureka Moments Shared by Chemists. Hints at Enhancing One’s Own Creativity (and Even One’s Joy)" by Jeffrey Seeman and Judy Wu
pubs.acs.org/doi/10.1021/...

#ChemSky

The starter pack the TPD community has been waiting for:

go.bsky.app/CE2P2Yh

I also know a podcast that's perfect for eureka moment stories @carolynbertozzi.bsky.social . Back of the Napkin would love to host you! #glycotime

Thanks to Alex for hosting me on Bio-techne's Back of the Napkin Podcast. We discuss my science, life as a scientist, what it's like to be a TED speaker, and how strong feelings of anger won me an NIH New Innovator Award. Hope it can be helpful if anyone needs some inspiration.

We are here now! ☺️

A conversation between two incredibly gifted communicators and quite frankly just good people. Can't wait to listen to this.

First Napkin on Bluesky! 🦋 Drug delivery extrodinare and science communication master @kwhitehead.bsky.social on #BackoftheNapkin 🎙 Katie sure nows how to cook up an innovative idea! open.spotify.com/episode/7jVY...

Thanks for the links Oliver. We also have some exciting new chem bio tools our R&D team having been working on which I'm excited to share soon!

Great list! Please can you add me too.

If you don’t feel stupid doing science, you’re not trying hard enough.