Predicted structural comparisons of cotton virus A (CotVA) open reading frames (ORFs) 2 and 4. A, Top: comparison of AlphaFold 3 (AF3) prediction (turquoise) over the Protein Data Bank crystal structure (3K4T, orange) of the cauliflower mosaic virus (CaMV) virion-associated protein (VAP). Bottom: structural alignment of the AF3 prediction of the CaMV VAP (white) with the AF3 prediction of CotVA ORF4 (colored). B, Same as A but seen from the bottom. C, Comparison of hydrophobicity of CaMV VAP (top), and the predicted hydrophobicity of CotVA ORF4 (bottom). D, Structural alignment of AF3 prediction of Cestrum yellow leaf curling virus (CmYLCV) aphid transmission factor (ATF) (white) to CotVA ORF2 (colored). Confidence in the predictions is measured in the B-factor field (temperature factor) colored from low confidence (cold colors) to high confidence (warm colors).
While investigating cotton leafroll dwarf virus in Mississippi,
Michael West-Ortiz et al. identified a novel virus, cotton virus A (CotVA), in Gossypium plants. Learn more: doi.org/10.1094/PBIO...
19.05.2025 19:34 — 👍 3 🔁 0 💬 0 📌 0
The Phytovirome | Phytobiomes Journal
The Phytovirome | Phytobiomes Journal apsjournals.apsnet.org/doi/full/10..... Schönegger et al. (2025) found that wild carrots harbored a greater diversity of virus taxa relative to farmed carrots and documented key differences in the virus communities of each host type.
03.04.2025 21:43 — 👍 3 🔁 3 💬 1 📌 0
Geographic distribution and relative abundance of Silhouette clusters among symptomatic plants. Sampling depth at each site is indicated by the size of the circle. Indicator amplicon sequence variants (ASVs) are listed for each Silhouette cluster according to Figure 3 and Supplementary Table S7.
Classical approaches to plant disease diagnosis assume one pathogen per disease. Betsy A. Alford et al. reexamine Fusarium oxysporum’s presumed role in chickpea wilt/yellowing on Ethiopian smallholder farms. Learn more: doi.org/10.1094/PBIO...
21.03.2025 18:02 — 👍 1 🔁 0 💬 0 📌 0
Comparison of microbial co-occurrence and network attributes associated with rhizospheres of infested versus uninfested plants across selection regimes and generations. Network attributes (e.g., average degree, modularity, # connector taxa, etc.) of each of the 21 networks, separated by insect treatment, are shown on the right. Bonferroni-adjusted results of six t tests comparing network attributes between aphid-infested and uninfested groups are inset within each top right panel (*P < 0.05, **P < 0.01), and the results of a generalized linear model of the number of connector taxa between insect treatments are presented in the bottom right panel (***P < 0.001).
Microbial communities support plant health but engineering them for pest resistance is complex. Using host-guided selection, Laramy Enders et al. reduced aphid damage on tomatoes by up to 20%, revealing key insights. Learn more: doi.org/10.1094/PBIO...
25.02.2025 17:29 — 👍 1 🔁 0 💬 0 📌 0
Six seed sterilization protocols tested on two varieties of maize. A, Examples of clean dead (top left), contaminated dead (top right), contaminated germinant (bottom left), and clean germinant (bottom right) B73 seeds. B, Overview of the six sterilization protocols used. Percentage of seeds that germinated and/or were contaminated with fungal growth for C, B73 seeds and D, Sugar Bun seeds for each of the different protocol variations. Letters indicate significant differences for clean germinants (Tukey honestly significant difference, P value < 0.05).
SynCom studies need seed sterilization to ensure gnotobiotic conditions. J. Jacob Parnell et al. tested six protocols on two maize varieties, finding sterilization requirements depend on genotype and seed source. doi.org/10.1094/PBIO...
11.02.2025 18:10 — 👍 0 🔁 1 💬 0 📌 0
Influence of soil extracts on the stimulatory effect on broomrape seeds. A, Relative germination ratios of broomrape seeds in presence of the conducive (CN) or suppressive (SN) soil extracts, either unfiltered or 0.22-µm-filtered at 101- or 102-fold dilutions, and in the presence, or not, of gluconasturtiin (10−6 M). Ratios are expressed as the number of germinated seeds on total seeds relative to the average ratio obtained with the positive control rac-GR24 (10−7 M), a synthetic strigolactone giving a positive germination ratio of 1 ≈ 65.20 ± 6.32% (median ± sd). B, Relative prehaustorium formation ratio of broomrape seeds in presence of the CN or SN soil extracts either 0.22-µm-filtered or unfiltered and at 101 or 102 dilutions. Ratios are expressed as the number of germinated seeds presenting prehaustoria on total germinated seeds relative to the average ratio obtained with the positive control TDZ (10−8 M), a synthetic analog of cytokinin, giving a positive prehaustorium formation ratio of 1 ≈ 95.33 ± 5.33% (median ± sd). Significance was determined using a nonparametric Kruskal-Wallis rank test and Dunn's Kruskal-Wallis multiple comparisons (P ≤ 0.05) and is indicated with different letters.
In Western France, rapeseed fields with reduced Phelipanche ramosa parasitism showed fungal-driven suppression. Suppressive soils hinder attachment and induce necrosis, with Berkeleyomyces linked to the effect. Lisa Martinez et al. highlight potential control strategies. doi.org/10.1094/PBIO...
07.01.2025 19:39 — 👍 0 🔁 1 💬 0 📌 0
