Alexandre D'Halluin

The endoribonuclease Rae1 from Bacillus subtilis cleaves mRNA upstream of stalled ribosomes Abstract. The ribosome-associated endoribonuclease 1 (Rae1) cleaves messenger RNAs (mRNAs) in a translation-dependent manner. Here, we identify a new Rae1

🎉 Very excited to see our latest research published in @narjournal.bsky.social !! 🎉

We unvieled the mechanistic of the RNase Rae1 in B. subtilis, cleaving mRNA upstream of stalled ribosomes at their tail end !

Congrats to all the teammates for the huge work ! ✨

academic.oup.com/nar/article/...

Conservation of sporulation genes and a transmembrane-containing Spo0B variant in Paenibacillus Sporulation is a strategy employed by many bacteria to survive harsh environmental conditions. The genus Paenibacillus includes spore-forming species notorious for spoiling pasteurized dairy products ...

Here we show that >90% of Paenibacillus encode a unique Spo0B with a transmembrane domain! TM domain helps it interact with its phosphorelay partners. Congrats to @isabellalin.bsky.social who left for grad school this week and her mentor @cassidyprints.bsky.social! www.biorxiv.org/content/10.1...

Conservation and evolution of the programmed ribosomal frameshift in prfB across the bacterial domain | mBio Translation termination is catalyzed by one of two release factors in bacteria, RF1 or RF2. It has been known for decades that RF2 levels in Escherichia coli are regulated by a programmed ribosomal frameshift within the prfB gene that encodes RF2. We ...

Latest from the lab! Analysis of everyone’s favorite regulatory mechanism in bacteria — the RF2 programmed frameshift! Likely present in the ancestor of bacteria, use of this mechanism is influenced by stop codon usage! Big congrats to @cassidyprints.bsky.social
journals.asm.org/doi/10.1128/...

Remodeling of RNA-binding proteome and RNA-mediated regulation as a new layer of control of sporulation | mSystems Understanding how bacteria survive extreme conditions is key to tackling challenges in health, food safety, and industry. This study reveals a previously unexplored layer of control in Bacillus subtil...

Thrilled to share my first last-author paper, just out in #mSystems! A work about #RBPs, #RNA and #sporulation in #subtilis.
Special thanks to my brilliant student T. Kaboré who's signing hist first PhD paper and to the whole team @galinier-lab.bsky.social
👉 journals.asm.org/doi/10.1128/...

We are also hiring a 4-year postdoc to study the dynamics and evolution of mobile genetic elements in the C-fixing Icelandic host-spring microbiomes

Join our exciting multidisciplinary team funded by @ukri.org BBSRC sLoLa

www.jobs.manchester.ac.uk/Job/JobDetai...

EMBL is back on hiring faculty! Check out the multiple open positions:

3 PIs- 2 in Heidelberg incl. our Molecular Systems Biology Unit, 1 in Grenoble
1 Head of Unit (Cell Biology & Biophysics, Heidelberg).

Closing deadlines in Sep!
+3 more positions to come out then, stay tuned...
@embl.org

Research position in Microbial Pharmacology and Population Biology (282139) | UiT The Arctic University of Norway Job title: Research position in Microbial Pharmacology and Population Biology (282139), Employer: UiT The Arctic University of Norway, Deadline: Wednesday, August 27, 2025

Feels surreal to say but: I'm hiring! If you are or know anyone interested in looking at the evolution of phage resistance using A. baumannii and natural transformation, then this might be for you
www.jobbnorge.no/en/available...

A rapid, simple, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies Nature Communications - Here, the authors present a method to rapidly isolate actively translating ribosomes in a time- and cost-effective manner using poly-lysine. The method is compatible with a...

Our RAPPL purification of ribosomes and associated material is out. I am really proud of this study in collaboration primary with Pavlovic-Djuranovic lab, as well as Jovanovic and Hashem Labs. This method aims to replace 60 years old purification of ribosomes by sucrose cushions

rdcu.be/ezlhg

A Mycobacterium tuberculosis Mbox controls a conserved, small upstream ORF via a translational expression platform and rho-dependent termination of transcription Magnesium (Mg2+) is vital for bacterial survival, and its homeostasis is tightly regulated. Intracellular pathogens like Mycobacterium tuberculosis (Mtb) often face host-mediated magnesium limitation,...

Sharing a new preprint thanks to @tinearnvig.bsky.social , Terry, Catherine and Declan !
We unveiled a dual mechanism of regulation of an Mtb Mbox riboswitch, combining translation regulation of a new conserved peptide and rho-dependent termination of transcription.
www.biorxiv.org/content/10.1...

Extracellular exosomal RNAs are glyco-modified - Nature Cell Biology Sharma, Jiao and colleagues report glycosylation of small RNAs within exosomes and propose a glycosylation-dependent mechanism for RNA targeting into exosomes, which may influence intercellular commun...

🍹Sharma, Jiao et al. report glycosylation of small RNAs within #exosomes and propose a glycosylation-dependent mechanism for #RNA targeting into exosomes, which may influence intercellular communication and RNA stability in the extracellular environment.
👉https://rdcu.be/eykKY
bit.ly/455vyuP

Frontiers to retract 122 articles, links thousands in other publishers’ journals to “unethical” network The publisher Frontiers has begun retracting a batch of 122 articles across five journals after an investigation found a network of authors and editors engaged in “unethical actions” such as manipu…

Frontiers to retract 122 articles, links thousands in other publishers’ journals to “unethical” network

Evolution of hunt, kill, devour in a Vibrio ecospecies Bacterial ecospecies are a recently recognized genetic structure that is hypothesized to arise by complex adaptation to a new niche. Using coadaptation analysis as a foundation for laboratory experime...

Happy to share the preprint from here in Shanghai, together with @danielfalush.bsky.social, as well as with team Zhizhou Jia, Huqi Wen, and Zhe Xie!

www.biorxiv.org/content/10.1...

Targeting the translation machinery: The RNase Y specificity factor Y-complex coordinates ribosome degradation https://www.biorxiv.org/content/10.1101/2025.06.27.661978v1

📢 Le CNRS lance sa campagne de recrutement !
+350 postes à pourvoir dans toute la France 🌍
Inscrivez-vous aux concours et participez à l’aventure scientifique. #RejoignezleCNRS
👉 carrieres.cnrs.fr/concours-ext...

#Recherche #Emploi

New preprint from our lab !

We have identify a new target of the endoribonuclease Rae1, flyY, and found that this RNase strictly cleaves its known targets 12nt upstream of stalled ribosomes, at their tailed end.

Mobile integrons encode phage defense systems Integrons are bacterial genetic elements that capture, stockpile, and modulate the expression of genes encoded in integron cassettes. Mobile integrons (MIs) are borne on plasmids, acting as a vehicle ...

🧪
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉

Short 🧵
www.science.org/doi/10.1126/...

Quality over quantity: Small RNA pauses translation elongation to lift protein activity Small noncoding RNAs typically regulate the translation initiation of target mRNAs and thereby protein production. In this issue of Molecular Cell, Thongdee et al. discovered a novel regulatory mechan...

Quality over quantity: Small RNA pauses translation elongation to lift protein activity: Molecular Cell www.cell.com/molecular-ce...

Formation of a membraneless compartment regulates bacterial virulence - Nature Communications The RNA-binding protein CsrA switches from upregulation to downregulation of virulence genes in bacteria through unclear mechanisms. Here, the authors show that the switch between promoting and repres...

Interesting study shows CsrA forms a dynamic, membraneless compartment with sRNAs, mRNAs & the degradosome in E. coli, flipping its role between activating and repressing virulence. A new layer of RNA-based regulation! #microbiology #RNA #CsrA

www.nature.com/articles/s41...

Remodeling of RNA-Binding Proteome and RNA-mediated regulation as a new layer of control of sporulation Sporulation allows certain bacteria to survive extreme conditions for extended periods posing challenges to public health and food safety. Transcriptional level of regulation relying on σ factors has ...

🚨 New preprint! 🚨
We just dropped our latest work on RNA-binding proteins and sporulation in B. subtilis! Turns out, the RBP landscape gets a major remix during sporulation 👀. Check it out here 👉 www.biorxiv.org/content/10.1...
#RBP #RNA #sporulation #Bacillus #microbiology @lcbofficiel.bsky.social

Congratulations to all the candidates who qualified for the #CNRS #CRCN competition in #Section21. Such brilliant science and incredible enthusiasm! Keep it up!

c3n-cn.fr/2025/01/31/c...

Transcriptomic profiling of individual bacteria by MATQ-seq - Nature Protocols Bacterial single-cell sequencing via MATQ-seq combines index sorting, random priming and rRNA depletion, and offers high cell retention and transcript capture rates, making it ideal for experiments wi...

If you want to do single-cell RNA-seq on your favourite bacterium, here‘s our detailed Nature Protocol for microbial MATQ-seq. It profiles up to 600 genes in a single bacterial cell!

www.nature.com/articles/s41...