Chris Toepfer

Assaying the myosin super-relaxed state across muscle types, cells and proteins for understanding muscle biology and use in drug discovery - Nature Protocols The Mant-ATP assay is a simple and accessible means for rapid quantitative assessment of the ratio of super-relaxed myosin to disordered relaxed myosin. This protocol provides a standardized methodolo...

Great collaborative work led by Christopher Toepfer and his team at the University of Oxford

@wellcometrust.bsky.social @thebhf.bsky.social @rdm.ox.ac.uk @carlsbergfondet.dk @greentempleton.bsky.social @medsci.ox.ac.uk @ox.ac.uk @loyolachicago.bsky.social @bristolmyerssquibb.bsky.social

We thank our group of committed and determined collaborators in Denmark and the US that have allowed this set of protocols to be brought together into a single place. Work made possible by funders@wellcometrust.bsky.social@rdm.ox.ac.uk@thebhf.bsky.social@carlsbergfondet.dk@greentempleton.bsky.social

A particularly novel part of this protocol never published before has been the approach to applying this technique in high-throughput in solution and in iPSC derived materials. Taking protocols from first steps through to data analysis and the final data appraisal.

This protocol has drawn together a series of teams working across tissues, cells, and molecules to bring together a compendium of approaches for this useful biochemical assay to make it simple for adoption.

We have today published a comprehensive set of protocols in
@natureportfolio.nature.com for assaying the myosin super-relaxed state across tissues, iPSC-CMs and molecules.
rdcu.be/eSysL

Some good news, myself and @simoesfilipa.bsky.social are advertising for a post-doctoral researcher to join us at the Oxford Organoid hub: tinyurl.com/OOHJob

Come and join us to develop novel human organoid systems as part of the MRC/BHF CRE in advanced cardiac therapies.

A joy to present at the Oxford @thebhf.bsky.social Centre of Research Excellence annual symposium. Great discussions and a great opportunity to show some of the research happening around the Oxford Organoid Hub. @rdm.ox.ac.uk @simoesfilipa.bsky.social @idrm.ox.ac.uk