Brian English

Janelia is hiring! "Invent new imaging methods, molecular tools, or protein chemistry approaches"

www.janelia.org/content/lead...

Today I departed from @hhmijanelia.bsky.social knowing that the next time I come here, I will be a visitor.

Thank you all for having me. I will miss you a lot.

Holiday party 2025 @hhmijanelia.bsky.social

Day 2 also covers Fourier Optics, with a fantastic lecture from Dr. Peter Kner! You'll never understand Fourier transforms better than you do after Peter explains it, trust us.

Apply now to join us and finally understand #Fourier space! bit.ly/49YQl7q

#microscopy

The Company of Biologists 100 logo to the left and QR code to the right. Portrait of Jennifer Waters to the left, text to the right 100 extraordinary biologists Jennifer Waters Jennifer Waters, a FocalPlane Scientific Advisory Board member, is the Director of Core for Imaging Technology & Education, Harvard Medical School, USA. She created MicroList and draws on over 25 years of teaching experience to create accessible microscopy education resources, including the online platform Microtutor. #100biologists #biologists100

We are highlighting Jennifer Waters, a @focalplane.bsky.social Scientific Advisory Board member, Director of CITE, Harvard Medical School, and creator of MicroList (now featured in FocalPlane) and Microtutor, as an extraordinary biologist. #100biologists
@jencwaters.bsky.social

Attempt number seven at uploading this video of intermediate filaments in an enormous COS7 cell. I have a feeling the BlueSky compression will not do it any favors.

4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods 4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.

We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt

We moved the AI@MBL course "Deep Learning for Microscopy Image Analysis" to HHMI Janelia (@hhmijanelia.bsky.social).

Join us for two weeks of intense lectures, exercise, and hands-on project work!

Course dates: June 4-18 2026
Application by: January 15 2026

www.janelia.org/you-janelia/...

Oh no!

New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇

www.biorxiv.org/content/10.1...

TODAY (Sunday Nov 9th) starting at 4pm. Please join me in an interactive performance at the Athenaeum in Alexandria VA #interactiveart #performanceart

Exciting draw and late @fcbayernmunich.bsky.social comeback against Union Berlin in der alten Försterei. See you next Saturday morning when we play Freiburg at home #miasanfamily♥️ #washingtondc #MiaSanMia

Fantastic and huge congratulations 🎉

A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....

Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....

Our confirmed count stands at 8.1 million participants worldwide for yesterday’s No Kings protest. Thank you to everyone who helped verify the numbers through our submission website. While some MAGAs attempted to submit false or misleading data (such as reporting “one person” for major cities)

Very excited to be co-organizing this meeting. Hope to see you there!

Maud Menten was born in Canada in 1879 and completed her UG and MD education at University of Toronto. As a research assistant with Leonor Michaelis in 1912, they wrote the classic paper describing the “Michaelis-Menten” model of enzyme kinetics.. #WomenInScience

Just heard him speak at Janelia just now. Wonderful talk!

#miasanfamily♥️

(Part 1) Hear from @hhmi_news Investigator David Stern about his research program and what he's uncovering in the lab.

Stern will join the Institute in February 2026, bringing his lab and HHMI appointment to Kansas City. @hhmi.org

Read more: bit.ly/4np96Fb

"Her work made it impossible to separate what we learn from our obligation to protect it. Generations of researchers have tried to follow that example." - Jennifer Doudna (2/2)

The culmination of many years work. One of the most advanced light-sheet microscopes in the world!

-> A large field of view Snouty light-sheet microscope, with any immersion remote refocus and real-time multi-angle projections!

For details see previous posts and here: github.com/amsikking/HT...

Congratulations Jie! @jiexiaolab.bsky.social

Stunningly beautiful RNA movies, congratulations!

🚀 Our new paper is out @natmethods.nature.com!

Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.

🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/

Nobel laureate Jennifer Doudna tells Jeffrey Goldberg that vaccinations have been so successful that "there's been a collective forgetting that measles, and mumps, and rubella—these used to be diseases that would kill people in fairly large numbers." #TAF25 bit.ly/46a5DUE

Today’s work, aside from meetings.

Head of Scientific Instrumentation Workshops EMBL is a world leader in scientific research and at the forefront in the development of cutting-edge technologies. It operates across six sites in Heidelberg (headquarters), Barcelona, Cambridge, Gre...

@embl.org Heidelberg is looking for a new Head of Scientific Instrumentation! This is an exciting opportunity at the interface between scientific research and technology development, overseeing our mechanical and electronic workshops!
embl.wd103.myworkdayjobs.com/en-US/EMBL/d...

Beautiful work led by @oliveringe.bsky.social. It was great fun to be involved with this project.

See Ollie’s paper and thread 👇

Thanks Misha for the shout-out!
It has been a truly mind-opening journey. WHOLISTIC is one step towards whole-organism neuroscience and a true physiological observatory - with extensions on the way! I am continually amazed by what the data reveals - so much remains to be discovered and mined.