Jim Dompierre

📢 Excited to share our new study in Nature Communications!
We show that the A-C linker safeguards centriole architecture & controls duplication. A big congratulations to Lorène for this beautiful discovery, which represents 4 years of her thesis work.
www.nature.com/articles/s41...

🚨 New EMBO Practical Course!
Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬
📅 20–24 Apr 2026 | 📍 EMBL Heidelberg
Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social

💡 Register your interest to get notified when registration opens:

🔗 www.embl.org/about/info/c...

New: Pasolli, Meiring, Akhmanova et al. @utrechtuniversity.bsky.social describe tools to acutely relocate #vimentin intermediate filaments by inducibly coupling them to microtubule motors. rupress.org/jcb/article/...

@joycemeiri.bsky.social @gijsjekoenderink.bsky.social
#Technology #Cytoskeleton

A multicellular star-shaped actin network underpins epithelial organization and connectivity - Nature Communications The combined role of cellular junctions and actomyosin networks in tissues remains unclear. Here, the authors identify a tissue-scale star-shaped network of actomyosin that preserves cell shape, limit...

Ever wished to become an actin star? 😎
Then try getting suspended above a non-adhesive substrate.
www.nature.com/articles/s41...

Lipid synthesis supports membrane growth and organelle biogenesis. Sunyoung Hwang, Eduardo Torres et al. @umasschan.bsky.social show that synthesis of sphingolipids, mainly long-chain bases, is essential to increasing nuclear membranes during #CellDivision. rupress.org/jcb/article/...

Registration of multiplexed images via actin staining in expanded cultured cells. (a) Composite image generated by registering two images acquired from the first and second imaging rounds. Phalloidin-labeled actin fibers were used as fiducial markers. The yellow box displays six-color images, including the DAPI channel. Gray, nucleus; red, actin; cyan, vimentin; blue, laminA/C; yellow, CCP; and magenta, cytokeratin 8/18. (b–c) Magnified views of the boxed region in a. Scale bars: (a) 2 µm; (b–c) 500 nm.

#ExpansionMicroscopy allows multiple rounds of staining & imaging, but requires high-quality registration of images between rounds. This study develops a registration technique using dense NHS-ester labels for multiplexed cyclic imaging in expandable tissue gels @plosbiology.org 🧪 plos.io/44M4wcX

Fig. 4 Super-resolution microscopy in live and fixed cells with Rhobin. (A) Rhobin transiently binds rhodamine molecules and thereby constantly replenishes signal lost through photobleaching of dyes. (B-D) No-wash STED microscopy of live U2OS cells transiently expressing LifeAct-Rhobin2 labeled with 100 nM JFX650. (C, D) Zoom-ins of regions highlighted in (B) imaged either with confocal or STED microscopy. Scale bar: 20 μm (B) and 10 μm (C, D). (E-H) Rhobin enables timelapse STED microscopy of fast ER dynamics with minimal signal loss. U2OS stably expressing N-terminal tag fusions of SEC61B were stained with 1 μM Halo-JFX650 (HaloTag7, reHaloF) or 2 μM SiRhP (Rhobin2) and imaged at a frame rate of 0.387 fps for at least 100 time points. (F, G) Selected frames from timelapse acquisitions of Rhobin2:SEC61B labeled with SiRhP (F) or HaloTag7:SEC61B labeled with Halo-JFX650 (G). Scale bar: 2 μm. (H) Signal .CC-BY-NC 4.0 International licenseavailable under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprintthis version posted June 25, 2025.;https://doi.org/10.1101/2025.06.24.661379doi:bioRxiv preprint 22 loss during timelapse imaging. Mean ± SD signal inside cells over time for indicated constructs. (I-K) PAINT-type super-resolution microscopy with Rhobin. Rhobin2-SEC61B was transiently expressed in U2OS cells and labeled with 5 nM SiRhP after chemical fixation. Imaging under no- wash conditions and near-TIRF illumination. (I) Repeated, but transient binding of SiRhP molecules to Rhobin2 at low nanomolar concentrations can be observed as intensity bursts in intensity time traces extracted from a diffraction-limited area. See movie S3 for raw data of binding-induced blinking. (J) Sum image across 10,000 frames of an image stack, simulating a diffraction-limited image. (K). Reconstructed image from localized molecules in the full stack.

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...

GitHub - aelefebv/nellie: Nellie: Automated organelle segmentation, tracking, and hierarchical feature extraction in 2D/3D live-cell microscopy Nellie: Automated organelle segmentation, tracking, and hierarchical feature extraction in 2D/3D live-cell microscopy - aelefebv/nellie

Try and share Nellie today!
We've included some sample data if you want to explore what Nellie can do.

Code: github.com/aelefebv/nel...
Paper: nature.com/articles/s41...

And please share your results with us! We're excited to see how you'll use it in your research.

🧵Fin/N

🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...

New study showing the development of pan-Expansion Microscopy by Morgan et al. @luskinglab.bsky.social to reveal the molecular composition and structural plasticity of individual nuclear pore complexes including LINC complex-dependent dilation. rupress.org/jcb/article/...

🔔 Rappel – AG de la SBCF, 5 juin 13h–16h30, Institut Cochin & en ligne 🔬

✨ Prix Jeune Chercheur 2025 – Marine Laporte
✨ Présentations jeunes chercheurs
✨ Bilan & approbation des comptes

🔗 Zoom : ID 966 7570 3089 | Code 547574
tinyurl.com/2my3e9fv

#SBCF #biologiecellulaire #jeuneschercheurs

Three full days of #ExM in Bordeaux. Onsite practical course on expansion microscopy. We will be sharing very soon the theoretical and application courses.

Ring deconvolution microscopy: exploiting symmetry for efficient spatially varying aberration correction - Nature Methods Ring deconvolution microscopy leverages symmetry to provide fast and straightforward spatially varying deblurring and offers improved speed and image quality compared to standard approaches across div...

Another super paper came out this week from Amit Kohli and Laura Waller on Ring deconvolution microscopy. This one is fairly technical, but essentially it's a computational approach that lets you do deconvolution when there is a spatially varying PSF. www.nature.com/articles/s41...

Figure 3: The effect of buffer composition on photobleaching. A field of view (FOV) was irradiated several times, with images taken at a regular interval. representative images are shown as well as the average fluorescence intensity traces of 8 FOVs normalized to the initial image. Standard deviations correspond to the light shaded regions. Panel A: Fixed U2OS Cell stained with Ab488-phalloidin imaged in buffer C or buffer ANice. Panel B: Living U2OS cells stained with concanavalin A-AF488 imaged in HBSS or HBSS supplemented with 8mM KI (blue).

A non-toxic, user-friendly buffer that enhances green fluorophore performance in conventional and super-resolution imaging by Wim Vandenberg and team:
www.biorxiv.org/content/10.1...

Join me May 20 @ 11 AM ET for a webinar with Bio-protocol!

I'll walk through our single-shot 20-fold expansion microscopy (20ExM) method + share the full protocol so you can try it too.

Le gouvernement annule 3,1 milliards d’euros de crédit pour les missions écologie, économie et recherche et enseignement supérieur Selon un décret publié samedi au « Journal officiel », ces annulations sont faites dans le cadre de l’effort supplémentaire de 5 milliards d’euros pour 2025.

"Choose France for Science!" *government cuts half a billion more to the research budget*

🚨🔬💗Whether investigating cell organelles or mapping proteins, together with Victor Puelles's lab we lay a roadmap for selecting optimal #ExM and #SuperResolution #microscopy combinations. Daria Aristova and Dominik Kylies review with amazing co-authors

pubs.aip.org/aip/apr/arti...

Happy #fluorescentFriday: dendritic spines of Purkinje cell after 10x expansion microscopie #expansionMicroscopie #ExM

Time to expand your vision! 👀🌱
Our latest work on ExM in plants is out
Back-to-back with the Danzl 's lab (@ISTAustria) led by Magali Grison @lbm-bordeaux.bsky.social with @monica-bic.bsky.social and @gmaucort.bsky.social from the @bic-bordeaux.bsky.social .

academic.oup.com/plcell/artic...

Root expansion microscopy: A robust method for super resolution imaging in Arabidopsis Root expansion microscopy (ROOT-ExM) achieves super-resolution expansion microscopy in plants.

Finally out!, after a few months of delay, our protocol on ExM in plant roots is now available in The Plant Cell
academic.oup.com/plcell/artic...
Beautiful images in a great collaboration of the @bic-bordeaux.bsky.social with @emmanuellebayer.bsky.social and Magali Grison @lbm-bordeaux.bsky.social

Functionalized Docetaxel Probes for Refined Visualization of Mitotic Spindles by Expansion Microscopy Visualizing the ultrastructure of mitotic spindles, the macromolecular machines that segregate chromosomes during mitosis, by fluorescence imaging remains challenging. Here we introduce an azido- and ...

🆕 Our new functionalized docetaxel probe enables superior visualization of dense mitotic spindle structures during cell division by expansion microscopy!

▶️ pubs.acs.org/doi/10.1021/...

Journalistes Français, il est temps de relayer ce qui se passe aux États Unis, c'est proprement terrifiant.

Informez, et faites que l'on se vaccine contre une dictature anti-science, illibérale et mortifère.

#MiFoBio2025 the autumn microscopy school by @gdrimabio.bsky.social will take place from 10th to 16th October 2025. The call for workshops to share pratical knowledges is open till 31/01 ateliers-mifobio.fr
We'll bring stuff as usual, stay tuned ! @cnrsingenierie.bsky.social

Figure 4

Using expansion microscopy alongside protein cycleHCR, the researchers unveil the complex network of 10 subcellular structures in mouse embryonic fibroblasts. (pg. 7) Figure 4 illustrates the application of cycleHCR to protein imaging with expansion microscopy, highlighting the detailed spatial...

High-resolution template matching of the human ribosome in situ by Lippincott-Schwartz Lab @hhmijanelia.bsky.social @hhmi.bsky.social. >40 conformations within together reveal molecular dynamics of the elongation cycle with previously unknown bending and “spring-like” motion.

Could you add me too please?

Did I miss any Blueskiers who are working on Expansion Microscopy? #UExM

go.bsky.app/Qxks9WD