Jim Dompierre

🧫 Amazing workshop, great mood at #Mifobio2025!
Julie Lesieur & Delphine Judith led “Understanding immune synapse micro-architecture” using expansion microscopy + 3D-STED. 🙌
➡️ Another session on Oct 15 — don’t miss it!

#Microscopy #STED #Mifobio2025
@gdrimabio.bsky.social

#Mifobio2025
@gdrimabio.bsky.social

First workshop of the week! Self-blinking dyes of Luke Lavis, with Luke himself, Eva Pinto, Sandrine Lévêque-Fort and the Abbelight @abbelight.bsky.social team.
#Mifobio2025
(I apologize for the horrible photos taken in an imaging school)

A Trypanosoma brucei cell visualized by confocal microscopy after ultrastructure expansion. The cell was immunolabelled for the BILBO1 protein (yellow) and stained with fluorescent NHS-ester (grey). The flagellar pocket is highlighted in pink. Credit: Marie Zelená

The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6

#Mifobio2025
Focus on the module 4 :
👉 Multi-scale imaging of biological systems

We are delighted to welcome David LABONTE
@imperialcollegeldn.bsky.social @jungeakademie.bsky.social

Lecture : “photogrammetry, synthetic data, and high-throughput automatic data extraction in insects”

#GDRimabio

#Mifobio2025
Focus on the module 4 :
👉 Multi-scale imaging of biological systems

We are delighted to welcome Anne BEGHIN @mbisg.bsky.social

Lecture - “Breaking scale: Image Analysis–Based AI to Streamline multilevels 3D quantitative Imaging and Tackle the vEM Challenge”

#GDRimabio

#Mifobio2025

Focus on the module 4 :
👉 Multi-scale imaging of biological systems

We are delighted to welcome @merlinlange.bsky.social
Institut de la Vision Paris

Lecture : “Building a Multimodal Atlas of Vertebrate Development”

👉 imabio-cnrs.fr/mifobio-2025/

#GDRImaBio #microscopy

#Mifobio2025

Focus on the module 4 :
👉 Multi-scale imaging of biological systems

We are delighted to welcome Professor Timo BETZ
@uni-goettingen.de

Lecture - “Flow, Force & Form : Multimodal 3-D Microscopy for Living-Tissue Mechanics

👉 imabio-cnrs.fr/mifobio-2025/

#GDRImaBio #microscopy

#Mifobio2025
Focus on the module 5:
👉 Physical measurements, control and handling -Mechanobiology

We are delighted to welcome Daria BONAZZI
@pasteur.fr @ijmonod.bsky.social

Lecture : “How Mechanical Forces Shape Bacterial Infections?”

👉 imabio-cnrs.fr

#GDRimabio #microscopy #biological_imaging

#Mifobio2025

Focus on the module 5:
👉 Physical measurements, control and handling -Mechanobiology

We are delighted to welcome Professor @ivatolic.bsky.social
Ruđer Bošković Institute

Lecture : “Mechanobiology of the Mitotic Spindle”

👉 imabio-cnrs.fr

#GDRimabio #microscopy #biological_imaging

#Mifobio2025

Focus on the module 5:
👉 Physical measurements, control and handling -Mechanobiology

We are delighted to welcome Professor @salaita.bsky.social @salaitalab.bsky.social

Lecture - “May the Force Be Measured : mechano probes reveal the molecular forces generated by cell

#GDRimabio

#Mifobio2025

Focus on the module 5:
👉 Physical measurements, control and handling -Mechanobiology

We are delighted to welcome Pierre NASSOY
@univbordeaux.bsky.social

Lecture : “formation, morphometrics and mechanics of epiblast models”

👉 imabio-cnrs.fr

#GDRimabio #TreeFrog_Therapeutics

#Mifobio2025
Focus on the module 2 :
👉 the challenges of spatial and temporal quantification

We are delighted to welcome Dr. Giuseppe VICIDOMINI @vicidominilab.bsky.social @iitalk.bsky.social

Lecture - “Photon-Resolved Microscopy : Image-Scanning Microscopy with SPAD Array Detector and Beyond”

#Mifobio2025

Focus on the module 3 :
👉 Analysis, simulation and modelling : a new paradigm with AI ?

We are delighted to welcome Wei OUYANG - Kungliga Tekniska högskolan

Lecture : “Generative AI for Microscopy : From Automated Imaging to Virtual Cell Modeling”

👉 imabio-cnrs.fr/mifobio-2025/

I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager!
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...

#Mifobio2025

Focus on the module 2 :
👉 the challenges of spatial and temporal quantification

We are delighted to welcome Anne Sentenac
@institutfresnel.bsky.social

Lecture : “Super-resolved fluorescence microscopy using Structured Illumination Microscopy”

👉 imabio-cnrs.fr/mifobio-2025/

#Mifobio2025

Focus on the module 2 :
👉 the challenges of spatial and temporal quantification

We are delighted to welcome @fbzt.bsky.social @impvienna.bsky.social

Lecture : “Introduction to MINFLUX Imaging and Tracking”

👉 imabio-cnrs.fr/mifobio-2025/

#GDRImaBio #microscopy #biological_imaging

#Mifobio2025

Focus on the module 2 :
👉 the challenges of spatial and temporal quantification

We are delighted to welcome Professor Joerg BEWERSDORF @yalesph.bsky.social

Lecture - “Single-molecule Localization Microscopy Techniques: Foundations and Cell Biological Applications”

#GDRImabio

#Mifobio2025

Focus on the module 1 :
👉 "Marking strategies at different scales of life"

We are delighted to welcome @michellefrei17.bsky.social
@ethz.ch

Lecture : "Chemigenetic kinase biosensors for multiplexed activity sensing and super-resolution imaging"

👉 imabio-cnrs.fr/mifobio-2025/

#Mifobio2025 - Focus on the module 1 "Marking strategies at different scales of life"

We are delighted to welcome Professor @amypalmerzinc.bsky.social @colorado.edu

🎤 Lecture : “Genetically encoded sensors and how to use them quantitatively to learn new biology”.

👉 imabio-cnrs.fr/mifobio-2025/

#Mifobio2025 - Focus on the module 1 "Marking strategies at different scales of life"

We are delighted to welcome Professor @michaelzlin.bsky.social

Lecture : “New dimensions in optical imaging: exploring space and time with fluorescent and bioluminescent proteins”

👉 imabio-cnrs.fr/mifobio-2025/

#Mifobio2025

Focus on the module 1 :
👉 "Marking strategies at different scales of life"

We are delighted to welcome Professor Marc Vendrell
@edinburgh-uni.bsky.social

Lecture : “Fluorescent probes for translational bioimaging”

👉 imabio-cnrs.fr/mifobio-2025/

🔬For 22 years, we have been organizing #Mifobio "Functional Microscopy for Biology" the @cnrs.fr thematic school.

🎓 Courses, Seminars, Round Tables, Workshops. The overall theme concerns #biological_imaging in its most interdisciplinary aspects.

👉 Program: imabio-cnrs.fr

#GDRImaBio #Mifobio2025

Göttingen Expansion Forum 2025 Live Stream YouTube video by Neuroscience and Beyond

#GEF25 LIVE BROADCAST

www.youtube.com/live/a_0X5Le...

We are delighted to welcome @abbelight.bsky.social to the
11th edition of #Mifobio the @cnrs.fr thematic school !

Rendez vous from 10 to 17 October 2025 in #Seignosse #France !

Four channel maximum intensity projection of an expanded NIH 3T3 fibroblast imaged with Zeiss Airyscan. DNA displayed in white. Mitochondrial outer membrane displayed in green. F-actin displayed in orange. alpha-tubulin displayed in purple.

Time to add some ExM to #FluorescenceFriday ! Sample description in alt-text.

Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy

• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets

spirochrome.com/product/hak-...

Pan-ASLM system for large scale expansion microscopy from the Bewersdorf lab.

www.biorxiv.org/content/10.1...

Our new preprint is about the airway epithelium microtubule network, cilia, basal body protein composition, averaging of volumetric fluorescence data, and expansion microscopy.
Four years of very hard work from our very talented Emma van Grinsven. www.biorxiv.org/content/10.1... (1/N)

Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social