Paul Conduit

We are looking to hire a postdoc to work on the regulation of microtubule nucleation in neurons. See advert attached. Contact me by email for any extra info.

haha, i paid with my time and feeling the pain of constructive criticism

We propose that the plus ends of MTs nucleated by g-TuRCs at the cis-Golgi are stabilised by CLASP at the trans-Golgi such that MTs grow along the cis-to-trans Golgi axis and thus grow towards and into axons rather than dendrites. This mechanism therefore helps maintain MT polarity in neurons.

Depleting CLASP or reducing the levels of g-TuRC at the Golgi perturb asymmetric microtubule nucleation towards the axon, causing more MT plus ends to grow towards and into dendrites (on the opposite side of the soma). This perturbs dendritic MT polarity, which is normally mainly minus-end-out.

We show that specific Golgi stacks within the soma are oriented cis-to-trans towards the axon entry site and that these Golgi stacks nucleate MTs towards the axon. We find that MT nucleating g-TuRCs and MT plus-end stabilising CLASP localise to the cis- and trans- Golgi compartments respectively.

In neurons, MTs in axons have their plus ends pointing away from the soma (plus-end-out) while many MTs in dendrites have their plus ends pointing towards the soma (minus-end-out). This differential polarity is important for correct cargo sorting, neurite identity, neuronal development and function

An image of class I da neurons in the head region of a Drosophila larva

Very happy to announce that our new paper on how asymmetric #microtubule nucleation from Golgi is required for #microtubule #polarity in #neurons has been published online in @currentbiology.bsky.social. An amazing effort from postdoc Akila Yagoubat. authors.elsevier.com/a/1kiDc3QW8S...

#JobOffer

💼 Molecular biology and drosophila engineer (M/F)
📅 February, 17th
📍 Institut Jacques Monod / @pconduit.bsky.social's Lab

Apply before February 6 ➡️ emploi.cnrs.fr/Offres/CDD/U...

#Biology #Engineer

Do you want to hear the latest and greatest about the neuronal cytoskeleton? Join us in Chile next April for #NeuroCyto2025, organized by @monicamsousa.bsky.social, Kassandra Ori-McKenney and Tomás Falzone! Registration is open until March 5: neurocytoskeleton.com/2025/

I think I might keep my new name 😂

Our lab has multiple open positions for post-docs to study the regulation and function of cytoplasm mechanics during embryo development.
Details below 👇👇
Please Repost !!!

@ijmonod.bsky.social @cnrs.bsky.social