Clausen Lab

Tissue-specific consequences of tag fusions on protein expression in transgenic mice Author summary To better understand the function of specific proteins, researchers need ways to quantify their abundance, to visualise their movements, to understand what other molecules they interact...

'In this manuscript, we show that tag fusions often change protein expression to a degree that is highly dependent on cell and tissue context. We characterise two underlying mechanisms, providing information that should help others to design more effective transgenic strategies'

www.nature.com/articles/d41...

On that note: hello world! My new lab on genetics of proteome remodeling is starting at the incredible MPI-CBG. We have big plans and open positions for PhD students and an RA. Apply now (!) at imprs.mpi-cbg.de and tinyurl.com/2vywj6f6 respectively.

wow, this looks amazing, in particular the reprogramming of oppA. next-level GCE. big congratulations to the whole team 👍👍

Just out in @science.org: Together with the lab of @wilhelmpalm.bsky.social, we used sequential in-vitro/in-vivo CRISPR screens to decipher metabolic adaptations in tumors. We find that acidosis is a dominant factor that shapes energy metabolism and stress resilience. www.science.org/doi/10.1126/...

congrats marco!! best of luck in the best of cities!

The proteasome-substrate-shuttle protein UBQLN2 contains—like other quality control system proteins—a long region devoid of lysine (a lysine desert)

Martin Grønbæk-Thygesen (from @rhp-lab.bsky.social) et al show that introducing K here causes ubiquitylation and degradation

doi.org/10.1101/2025...

Nobel Laureate Professor Sir John Gurdon dies aged 92 It is with great sadness that the University shares the news of the death of Professor Sir John Gurdon, founder of the Gurdon Institute.

Science has lost a GIANT and the world has lost one of the kindest most inspiring people I had the pleasure of knowing. Just an extraordinary man whose curiosity was infectious and whose dry wit will always be memorable- RIP John

www.cam.ac.uk/research/new...

Allosteric control of the bacterial ClpC/ClpP protease and its hijacking by antibacterial peptides
Axel Mogk, Marta Carroni et al
www.embopress.org/doi/full/10....

A Leishmania virulence factor harnesses an allosteric kinase switch to regulate its ubiquitin ligase activity Stringent control of ubiquitylation is a central requirement of signaling specificity in eukaryotes. Here, we discover a domain module integrating pro…

Allosteric activation of a ubiquitin ligase by an internal kinase domain! Congratulations to our team member Thornton Fokkens for this fascinating discovery in a neglected disease area with strong therapeutic need. Many thanks to all our great collaborators!
www.sciencedirect.com/science/arti...

Spectacular work. Absolutely spectacular. Go check out the paper. It truly is #lipidtime now

so well deserved

Dear scientific community,

I am looking for two postdoctoral structural biologists to join my group at the Department of Biochemistry, University of Oxford.

my.corehr.com/pls/uoxrecru...

my.corehr.com/pls/uoxrecru...

The Vienna BioCenter PhD program in Molecular BioSciences will offer 20+ fully-funded positions in the Autumn Call which is now open. Please share. Further details: www.vbcphd.at

Our Wadjet-II paper by @roisnehamelinf.bsky.social is out in polished form: www.cell.com/structure/fu...

With a great preview by @kevincorbett.bsky.social & @amardeeep.bsky.social highlighting the potential of Wadjets:

Wadjet—Keeping a watchful eye on circular DNA.

www.cell.com/structure/fu...

WARM congratulations to: Tom DENDOOVEN, @martinpacesa.bsky.social, @mschuhmacher.bsky.social , @lfmilles.bsky.social, @preinerin.bsky.social,Markus Hopfler, @charleneboumendil.bsky.social, Gergo Gogl, @shifralansky.bsky.social, @alexanderbelyy.bsky.social,Erik Benson, @dbojar.bsky.social! 🤩 #ERCStG

Job opportunity: come join the excellent and friendly #ProteostasisUK community!

I’ve already highlighted this landmark #lipidtime paper by @nadlerlab.bsky.social & colleagues, but ICYMI, do read André’s thread — this study is a huge leap forward in understanding the logic of intracellular lipid flux & is a phenomenal example of #chembio probes 🤝 quantitative imaging 🤝 modeling!

A split-site E3 ligase mechanism enables ZNFX1 to ubiquitinate and cluster single-stranded RNA into ubiquitin-coated nucleoprotein particles Grabarczyk et al. show the structure and mechanism of a non-canonical ubiquitin ligase, which is activated through nucleic-acid-induced oligomerization and is critical for cell survival during immune ...

Happy to share our work on the structure and function of the unusual E3 ligase ZNFX1 @cp-cell.bsky.social. It uses a nucleic acid-activated transthiolation mechanism, ubiquitinating and clustering RNA to protect cells in an immune response. @clausenlab.bsky.social
www.cell.com/cell/fulltex...

Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org‬: www.embopress.org/doi/full/10....

Co-translational ribosome pairing enables native assembly of misfolding-prone subunits - Nature Communications Protein complexes are essential for cell function. Here, authors show that paired ribosomes can help each other’s nascent chains fold correctly, enabling proper dimer assembly and preventing misfoldin...

Co-translational ribosome pairing enables native assembly of misfolding-prone subunits. www.nature.com/articles/s41...

A widespread family of viral sponge proteins reveals specific inhibition of nucleotide signals in anti-phage defense Chang et al. discover anti-CBASS 4 (Acb4), a family of viral sponges that inhibits bacterial immunity by sequestering nucleotide immune signals. Acb4 homologs in phages that infect hosts across all ma...

The beautiful, ever-expanding universe of viral proteins targeting nucleotide immune signals!

Paper by @reneechang.bsky.social in @cp-molcell.bsky.social on a nucleotide sponge www.cell.com/molecular-ce...

and preprint by @doudna-lab.bsky.social on viral nucleases www.biorxiv.org/content/10.1...

:) agreed!!

Quantitative imaging of lipid transport in mammalian cells Nature - Directional, non-vesicular lipid transport is responsible for fast, species-selective lipid sorting into organelle membranes.

big one! a decade in the making. nadler & team develop tools to track lipid transport in living cells at single-species resolution. turns out non-vesicular, asymmetry-driven transport is the main architect of organelle identity. feels like the dawn of a golden era for lipid biology. rdcu.be/eBGJv

wow - what amazing tools and fantastic work. era of lipid biology ahead. big congratulations to the whole team.

The small GTPase Ran defines nuclear pore complex asymmetry Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!

Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!

Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

Cryo-EM structures of Egl-BicD-RNA complexes reveal how diverse mRNAs are selected for subcellular localization Localization of mRNAs is a widespread mechanism for controlling where proteins operate in cells and underpins many fundamental processes, from embryonic patterning to synaptic plasticity. This spatial...

www.biorxiv.org/content/10.1...

beautiful work. big congrats.

Excited to share our latest work with @simonbullock11.bsky.social! We looked at how diverse mRNAs get selected for subcellular localization and it turns out that a single protein can recognize different RNA elements using shared features that weren’t apparent before.
www.biorxiv.org/content/10.1...

In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells In eukaryotic cells, LINC complexes physically bridge the two nuclear membranes to cytoskeletal filaments, transmitting mechanical forces to the nucleus. Due to their dynamic and membrane-embedded nat...

Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo

www.biorxiv.org/content/10.1...

thank you so much, marcin, for the warm welcome in orleans. it was a pleasure to visit the CBM institute, get inspired by your science and share memorable time with family and friends. already looking forward to the next time.