Marvin Tanenbaum

A fantastic effort by first authors Huib Rabouw, @janinschoko.bsky.social, @michamuller.bsky.social with lots of help in image analysis by @baarsmatthijs.bsky.social and @jakobpueschel.bsky.social. Also a great collaboration with the groups of @hansclevers.bsky.social and Ron Fouchier!

Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity In this study, an imaging technology, “VISUN,” is developed that allows live-cell visualization of unmodified influenza virus during infection with single-vRNP resolution. Combining VISUN with multipl...

Out now in Cell Systems: We develop a technique to visualize influenza A virus in live cells with single viral RNA resolution. Combined with precise readouts of viral transcription, we map the whole influenza A viral life cycle and identify key bottlenecks in infection.

www.cell.com/cell-systems...

Pre-assembly of biomolecular condensate seeds drives RSV replication Nature - Viral ribonucleoprotein–viral protein networks form pre-replication centres that nucleate viral factories and drive respiratory syncytial virus replication.

Now out in Nature! We visualize infection of the RNA virus RSV in real-time with single-vRNP resolution to understand how RSV establishes viral factories, biomolecular condensates that act as sites of viral replication. A huge collaborative effort led by Dhanushika Ratnayake!

rdcu.be/e1bBW

Excited to share our new paper! We developed a method to visualize proteasomal degradation at the single–molecule level in live cells, enabling us to dissect distinct modes of substrate engagement, probe co-factor dependence, and study proteasome–ribosome collisions.
www.biorxiv.org/content/10.6...

The project was led the very talented graduate student @maxmadern.bsky.social and we received a lot of important advice on the proteasome from our collaborator David Haselbach

New lab paper!! We develop a technology for real-time, single-molecule visualization of proteasomal substrate degradation in cells. We find that the site of substrate engagement by the proteasome determines decay kinetics, efficiency and co-factor requirement.

www.biorxiv.org/content/10.6...

Curious about where, how and when #extracellularvesicles fuse with target cells?

A new methodology developed in our group, spearheaded by Jasper van den Ende, provides insight in this process.

EV-FUSIM: an EV-Fusion Spatiotemporal Imaging Method.
www.biorxiv.org/content/10.1...

The Hubrecht Institute has an open call for it's PhD program. It's a great place to work and do research, so apply!

Wow, very interesting!

viruses?

Prkra dimer senses double-stranded RNAs to dictate global translation efficiency Prkra serves as a primary dsRNA sensor in pluripotent cells, restricting RNA virus replication. By dimerizing through dsRBD3, it sequesters eIF2 complexes from the translation machinery and compensate...

Really nice paper on a new sensor for double-stranded RNA:

Prkra dimer senses double-stranded RNAs to dictate global translation efficiency: Molecular Cell www.cell.com/molecular-ce...

Can anyone recommend good mRNA therapeutics conferences that are coming up? Ideally conferences that are attended by both industrial and academic researchers?

It's possible to apply for the Hubrecht Talent Program again!
The HTP aims to promote scientific excellence in the Netherlands by supporting talented minority students in pursuing a career in scientific research.

Read more in the flyer and on www.hubrecht.eu/about-us/hub...

Marvin Tanenbaum Marvin Tanenbaum is developing revolutionary imaging techniques that allow him to film molecular processes in living cells at the level of individual biomolecules.This provides fundamentally new insig...

Very honored to receive the Ammodo Science Award for fundamental research! Great recognition for the amazing work done by all our group members, both past and present, over the past 10 years! Also a huge congrats to my co-awardee Leila Akkari!

www.ammodo-science.org/researches/m...

Sounds really exciting!

Amazing work led by Dhanushika Ratnayake, and a huge thanks to the large group of fantastic collaborators!

Pre-assembly of biomolecular condensate seeds drives RSV replication During infection many RNA viruses, including respiratory syncytial virus (RSV), form specialized biomolecular condensates, inclusion bodies (IBs), where viral transcription and replication occur[1][1]...

Very excited to share a new paper from the lab! We visualize RSV infection with single-vRNP resolution and show how the virus coordinates transcription and replication with the formation of viral factories, biomolecular condensates that act as sites of viral genome control.

doi.org/10.1101/2025...

Save the date for the next edition of this wonderful meeting. We look forward to seeing you in Portugal!!

The critical role of enterovirus 2A protease in viral translation, replication, and antagonism of host antiviral responses Enteroviruses dramatically remodel the cellular infrastructure for efficient replication and curtailing host antiviral responses. Proteases 2Apro and 3Cpro have been implicated in these processes base...

Check out the latest paper from our collaboration with the Kuppeveld lab where we use a combination of virology and single-molecule imaging assays to reveal the role of the enterovirus 2A protein:
www.biorxiv.org/content/10.1...

Super happy to receive a VICI grant from NWO! 5 more years of studying mRNA translation using single-molecule imaging

This is also our experience. Photobleaching of FPs is often reported at low excitation power (e.g. LED based widefield), and it is often unpredictable how such FPs will behave at high power, for example when using a different microscope modality

Wow, very nice!

Amazing work lead by @maxmadern.bsky.social and Sora Yang, and a very nice collaboration with @mariannebauer.bsky.social and Olivier Witteveen. @hubrechtinstitute.bsky.social

Long-term imaging of individual ribosomes reveals ribosome cooperativity in mRNA translation Ribosomes cooperate through transient collisions to ensure efficient translation.

Our paper on Stopless-ORF Circular RNAs (socRNAs) is now out in Cell. By high-res tracking and comparing translation by either single or multiple ribosomes, we find that ribosomes cooperate to overcome pausing to ensure fast and efficient translation

www.cell.com/cell/fulltex...

Wow, mindblowing!

Very happy to share our preprint on visualizing the life cycle of Influenza viruses using single-molecule imaging! 🥳 We developed two techniques to visualize infections of unmodified influenza viruses in live cells from endosomal release to budding of new viruses. For more details&videos see below ⬇️

janinschoko.bsky.social PhD student in the Tanenbaum Lab at the Hubrecht Institute, long-term fan of live-cell imaging, now infected by Influenza A research

Janin Schokolowski now on bluesky too: @janinschoko.bsky.social

Fantastic, multidisciplinary team effort by Huib Rabouw, Janin Schokolowski and @michamuller.bsky.social with enormous help from @baarsmatthijs.bsky.social and Jakob Puschel and great collaborations with @hansclevers.bsky.social's and Ron Fouchier's groups!

Mapping the complete influenza A virus infection cycle through single vRNP imaging Cell-to-cell heterogeneity is a common feature of viral infection that can generate enormous complexity, complicating understanding of infection progression and interpretation of differences between v...

Our new paper is out: "Mapping the complete influenza A virus infection cycle through single vRNP imaging". Combining newly-developed single-molecule imaging approaches with in situ viral transcriptomics, we identify numerous non-canonical infection pathways.

www.biorxiv.org/content/10.1...

I love this paper!