Dimitrios L. Wagner

Rotation PhD student @emiliedevet.bsky.social in our lab made the Dish soap protocol work (on the first attempt) for a nuclear target with Dawn (the US version of dreft/fairy ) and thereby extended the dish soap protocol to a version for across the atlantic 🤩 @tothovalab.bsky.social #hemesky

Distinct EBV-Associated Phenotypes Due to a Novel Homozygous Missense Variant in CD27. https://www.biorxiv.org/content/10.64898/2026.01.15.698722v1

Genome-scale perturb-seq in primary human CD4+ T cells maps context-specific regulators of T cell programs and human immune traits Gene regulatory networks encode the fundamental logic of cellular functions, but systematic network mapping remains challenging, especially in cell states relevant to human biology and disease. Here, ...

Together with @ronghuizhu.bsky.social, we are thrilled to present our new perturb-seq study of 22M primary CD4+ T cells, across donors and timepoints – the result of a decade-long collaboration between the Marson @marsonlab.bsky.social and Pritchard @jkpritch.bsky.social labs 🧵 tinyurl.com/gwt2025

Terrific to have our paper, led by @tjflemin.bsky.social, featured as a plenary article in @bloodjournal.bsky.social today: ashpublications.org/blood/articl...

The resting and ligand-bound states of the membrane-embedded human T-cell receptor–CD3 complex Nature Communications - The T-cell receptor (TCR) activation mechanism has remained uncertain. Here, the authors present molecular structures for the apo and ligand-bound human TCR–CD3...

🚨This week in @natcomms.nature.com: We resolve an enduring enigma in T cell biology—how TCR binding to p/HLA triggers intracellular signaling. CryoEM in native environment reveals a 'jack-in-the-box' mechanism! Collab btw
@rockefeller.edu @mskcancercenter.bsky.social
rdcu.be/eUPPl

Clinical development of gene edited tacrolimus-resistant Treg (FKBP12KO-Treg) to enable simultaneous immunosuppression and support of immune regulation https://www.biorxiv.org/content/10.64898/2025.12.10.693159v1

Mutant NPM1 Maintains the Leukemic State through HOX Expression Brunetti et al. show that specific loss of NPM1c from the cytoplasm leads to downregulation of HOX genes and differentiation in NPM1 mutant AML. Blocking NPM1c nuclear export by XPO1 inhibition reduce...

Original work here ⬇️, led by the inimitable Lorenzo Brunetti and Michael Gundry. This work became foundational in the field.

Today's the print release of a #RibackLab and #GoodellLab collaboration— we’re excited to share the visual story behind the science, including the dramatic artwork that inspired how we thought about aberrant function of NPM1c - which occurs in about 1 in 3 adult acute myeloid leukemias (#AML).

A big advance, folks, for off-the-shelf, universal, engineered (base-edited) T cells achieving complete response vs incurable leukemia in several patients
www.nejm.org/doi/full/10....

Our case report of a patient developing drug-induced systemic sclerosis after bleomycin exposure – still showing antibodies after 5 years. Nicely done Philipp 🎉 #rheumsky

authors.elsevier.com/sd/article/S...

Extrusive and cohesive cohesin cooperate to repair double-strand breaks in DNA @science.org @albertomarin.bsky.social @gerlichlab.bsky.social @imbavienna.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...

Big news! Our team developed Neuro-PERC, a non-viral CRISPR delivery platform for genome editing of the brain. In our new preprint - bit.ly/NeuroPERC_pre - we report efficient editing in multiple models & species, including field-first results in large animals (pigs) 🧵👇

Super cool! Congrats! Have you looked into immune responses towards Cas proteins and (Neuro-)PERC peptides?

So now I’m wondering what you’ve been reading 😉

On this #WorldAIDSday @cgaebler.bsky.social, Olaf Penack & teams at #CharitéBerlin report the #SecondBerlinPatient cured of #HIV after heterozygous CCR5Δ32 HSCT. The study opens exciting perspectives for immune-based #HIVCure strategies for longterm ART-free viral control and ultimately cure.

Today’s xkcd made me cry.

In a good way.

xkcd.com/3172/

Workflow of EBV-seq

Adapting the 10x workflow to detect EBV reads, Younis et al show that EBV-transcribing B cells are increased in SLE patients, especially among CD27+CD21low B cells. They have autoreactive BCRs and are more poised for antigen-presentation.

2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...

CD8 T cells produce IFNg and CXCL13 in TLS

What mechanisms drive checkpoint inhibitor-associated interstitial nephritis (ICI-AIN)?
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.

www.biorxiv.org/content/10.1...
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#Nephsky 🧪

A monoclonal antibody that inhibits the shedding of CD16a and CD16b and promotes antibody-dependent cellular cytotoxicity against tumors @natcomms.nature.com
www.nature.com/articles/s41...

Congrats Jacob! Enjoy your time there and looking forward to the creative science it will inspire :)

(1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.

Ubiquitinylation is more complex than just a signal for proteasomal degradation: Linear ubiquitinylation of Methionine-1 is important for NFkB signalling.
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2

Cell-stereotyped DNA repair outcomes are widespread during genome editing Genome editing outcomes are governed by DNA repair pathways that vary with cell type and state. We developed scOUT-seq (single-cell Outcomes Using Transcript sequencing), a scalable approach that join...

Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...

A hitchhiker’s guide to the galaxy of space immunology Buck scientists help define the scope of Astroimmunology   With the advent of commercial spaceflight, an increasing number of people may be heading into space in the coming years.  Some will even get ...

A new subspecialty takes off! Astroimmunology is focused on the effects of #spaceflight on the #immunesystem and defines avenues for countermeasures. A bonus: the work will also inform research on #aging @natrevimmunol.nature.com evimmunol.nature.com www.buckinstitute.org/news/a-hitch...

I hope you still continue with the memes and stupid jokes 🙏

BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)

Enhancer-promoter compatibility is mediated by the promoter-proximal region Gene promoters induce transcription in response to distal enhancers. How enhancers specifically activate their target promoter while bypassing other promoters remains unclear. Here, we find that the p...

What is a promoter? And how does it work?

We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters

www.biorxiv.org/content/10.1...

Honored to be part of the 2025 #STATWunderkinds! Grateful for the many supportive mentors, colleagues, and friends with special shoutout to @bkleinstiver.bsky.social.

Full list: www.statnews.com/wunderkinds/

@statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social

Super cool! congrats Connor! 🥳

“ ‘Off the shelf’ Treg may soon be… on the shelf. “ - awesome quote by Prof. Fadi Issa.

Check out our new story: 👇
www.nature.com/articles/s41...

One week after the Nobel prize for Treg 🍀