Dimitrios L. Wagner

Cell-stereotyped DNA repair outcomes are widespread during genome editing Genome editing outcomes are governed by DNA repair pathways that vary with cell type and state. We developed scOUT-seq (single-cell Outcomes Using Transcript sequencing), a scalable approach that join...

Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...

A hitchhiker’s guide to the galaxy of space immunology Buck scientists help define the scope of Astroimmunology   With the advent of commercial spaceflight, an increasing number of people may be heading into space in the coming years.  Some will even get ...

A new subspecialty takes off! Astroimmunology is focused on the effects of #spaceflight on the #immunesystem and defines avenues for countermeasures. A bonus: the work will also inform research on #aging @natrevimmunol.nature.com evimmunol.nature.com www.buckinstitute.org/news/a-hitch...

I hope you still continue with the memes and stupid jokes 🙏

BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)

Enhancer-promoter compatibility is mediated by the promoter-proximal region Gene promoters induce transcription in response to distal enhancers. How enhancers specifically activate their target promoter while bypassing other promoters remains unclear. Here, we find that the p...

What is a promoter? And how does it work?

We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters

www.biorxiv.org/content/10.1...

Honored to be part of the 2025 #STATWunderkinds! Grateful for the many supportive mentors, colleagues, and friends with special shoutout to @bkleinstiver.bsky.social.

Full list: www.statnews.com/wunderkinds/

@statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social

Super cool! congrats Connor! 🥳

“ ‘Off the shelf’ Treg may soon be… on the shelf. “ - awesome quote by Prof. Fadi Issa.

Check out our new story: 👇
www.nature.com/articles/s41...

One week after the Nobel prize for Treg 🍀

“Good things take time”

Allogeneic Treg do not prevent their own rejection in immunocompetent hosts - HLA matching or HLA editing required for optional function!

A beautiful collaboration with Fadi Issa & Joanna Hester’s team @ox.ac.uk. @dlwagnerlabs.bsky.social

HLA matching or CRISPR editing HLA-I/II enables engraftment & function of allo human Treg therapy in a humanized mouse transplantation model @natcomms.nature.com @dlwagner.bsky.social @bcmhouston.bsky.social
www.nature.com/articles/s41...

@dlwagnerlabs.bsky.social on tour! 🌍🇩🇪🇺🇸

We had a great time at the Annual Congress of the European Society for Gene and Cell Therapy #ESGCT2025 in Sevilla. 🇪🇸

Super proud of everyone who presented their projects & grateful for working with this amazing team: science and good vibes! 🧬🧪💙

Accurate.

#academia #academy #philsky #philosophysky #philosophy #science

TCR activation impairs CAR-T cytotoxicity against separate target cells https://www.biorxiv.org/content/10.1101/2025.10.07.680634v1

Our team is on their way to the ESGCT conference 2025! Excited for this upcoming week full of science, talks, and inspiring discussions with the cell & gene therapy community!
Starting with the HDR workshop - talks by Isabell (‘one-pot’ PASTA) & Viktor
(BEKI) + posters by Ana, Lily, Jonas and Laura

Excited to share our preprint📯:
We introduce #IntegrateALL, an open-source RNA-seq pipeline for interpretable molecular diagnostics in acute lymphoblastic leukemia!🧬
Big thanks to Nadine Wolgast & team @ www.uksh.de
, catchall-kfo5010.com!
See: doi.org/p7k5 and 🧵

Big shoutout to the whole group who made #IntegrateALL possible - especially @alinamh.bsky.social, Thomas Beder and Claudia Baldus at www.catchall-kfo5010.com

Potentiating CAR-T bystander killing by enhanced Fas/FasL signaling mitigates antigen escape in heterogeneous tumors https://www.biorxiv.org/content/10.1101/2025.09.22.677496v1

Check out one of our latest stories about combining our non viral knock-in strategy with integrases for an efficient, targeted integration of transgenes >8kb in human T cells!

‘One-pot’ PASTA: programmable and site specific transgene addition - all in just one reaction 🫕

This has been such a great collaboration with @dlwagnerlabs.bsky.social! You really need to check it out

One-pot PASTA 🍝 - a one-step protocol to knock-in large transgenes.
I feel tempted to think about one-pot PIZZA 🍕 next!

First authors Isabell and @therealjonaskath.bsky.social were amazing!!

Super-large targeted Knock-Ins in primary T cells, with multigene inserts >8kb, in a single reaction. HOW?? 🙀

Combine simple CRISPR-HDR & Integrases for one-pot PASTA🍝

= Programmable And Site-specific Transgene Addition. 😎

Teamwork GIF ALT: Teamwork GIF

Led by the integrase lover Isabell Kassing (lawyer turned biologist) and our one and only @therealjonaskath.bsky.social. Amazing collab with @danielibrahim.bsky.social who pushed this idea. Lots of help from the @dlwagnerlabs.bsky.social and Alessa Ringel!

❤️

We found that the serine integrase Pa01 can be improved by adding a nucleus localization signal at the c-terminus -a small change that lifts Pa01 en par with the evolved Bxb1 variant from the Liu lab (eeBxb1). Engineered integrases boost 'one-pot' PASTA 🍝🛠️🚀

'one-pot' PASTA is more efficient than non-viral HDR and also better than PASSIGE with large constructs in primary human T cells. 🔬🚀

Super versatile; exchanging just nuclease + template enables you to put your cargo in a different place. 📍🗺️

'one-pot' PASSTA builds on 4 simple reagents that are easy to source & GMP compatible!

1. CRISPR-cas nuclease
2. HDR template (for landing pad insertion)
3. serine integrase mRNA
4. plasmid with cargo

-> a single electroporation in proliferating T cells =
✨ Efficient integration of XL-genes ✨

Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. H...

You like engineering T cells, but you are missing the right flavor?

Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1

We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪

🚀 In preparation for releasing two new stories, @therealjonaskath.bsky.social is revisiting the journey so far—sharing the lab’s last six papers.

What a ride it’s been… 🙌🧬🧪🧫

Next-generation multiplex-edited CAR-NK cells: more edits, more power? First clinical trials demonstrated the safety of adoptive cell transfer with allogeneic natural killer (NK) cell products from healthy donors, making them an attractive candidate for ‘off-the-shelf’ c...

🚨 Paper alert!
"Next-gen multiplex-edited CAR-NK cells: more edits, more power?" @jitc @bmj.com
With @dlwagner.bsky.social we discuss Wang et al.’s landmark paper on multiplex base-edited non-viral CAR-NK cells. Promising efficiency but safety challenges! jitc.bmj.com/content/13/9...

Plasmacytoid dendritic cells can transition to become cDC2s under to influence of TNF, while type-I interferon reduces TNFR expression and thereby inhibits transition. These transitioned cDC2 seem fully functional. 🧪 ⑂

Hornero et al 2025 in Nat Immunol
www.nature.com/articles/s41...

23 tools to work with (single-cell) TCR/BCR-seq immune repertoire data 🧵 👇