PULAK GHOSH

Template-independent enzymatic functionalization of DNA oligonucleotides with environment-sensitive nucleotide probes using terminal deoxynucleotidyl transferase Abstract. Given the emerging use of terminal deoxynucleotidyl transferase (TdT) in biotechnology and its clinical potential as a cancer marker and target,

Happy to share that one of my PhD work now in Nucleic Acids Research. We developed a fluorescent technique to detect the TdT activity, which often overexpress in leukemia cell. Grateful to my collaborator and my PhD supervisor Prof. S. G. Srivatsan. @IISER Pune.
academic.oup.com/nar/article/...

Structures of a DNA Polymerase Caught while Incorporating Responsive Dual‐Functional Nucleotide Probes Environment-sensitive C5-heterocycle-modified nucleotide probes serve as excellent substrates for Thermus aquaticus DNA polymerase (KlenTaq). The probes provided unprecedent insight into the chemical...

Happy to share our recently published Angew Chem article describing the mechanism of incorporation of modified nucleotides by DNA polymerases using fluorescence, 19F NMR and X-ray. Thanks to my Supervisor Prof.S.G.Srivatsan and congratulations to all authors!
onlinelibrary.wiley.com/doi/10.1002/...