John Tregoning

Comparative cross-species transcriptomics during RSV infection identifies targets to treat RSV disease Respiratory syncytial virus (RSV) remains a health threat to young children worldwide. The host immune response plays a key role in disease following infection. Infection models advance our understand...

Our new paper out with @chrischiulab.bsky.social

www.journalofinfection.com/article/S016...

Violets are blue
Roses are Red
You’d better buy Live Forever
If you don’t want to get dead
www.amazon.ie/Live-Forever...

Modifying non-coding regions of linear DNA vaccines to explore the interplay of expression and inflammation in immunogenicity - PubMed The COVID-19 pandemic highlighted the need for rapidly deployable, flexible vaccine platforms; particularly RNA which is now being explored for several other pathogens. DNA vaccines have potential advantages over RNA, including cost of manufacture, ease of storage and potentially lower reactogenicit …

Roses are Red
Violets are Free
We changed the vaccine response
By adding CpG
#academicvalentines
pubmed.ncbi.nlm.nih.gov/39829233/

Manipulating the delivery and immunogenicity of DNA vaccines through the addition of CB[8] to cationic polymers - PubMed Challenges with vaccine reactogenicity, stability, and access have highlighted the need to develop alternative strategies for formulation and delivery. We explored the incorporation of cucurbit[n]urils (CBs), as supramolecular "hosts," into nucleic acid-polymer polyplexes. CBs are small, non-toxic, …

Roses are Red
Violets are Great
You can improve DNA vaccines
By adding CB[8]
#academicvalentines
pubmed.ncbi.nlm.nih.gov/40978528/

Intranasal delivery of mRNA expressing newly identified Acinetobacter baumannii antigens protects against bacterial lung disease - PubMed Vaccines are central to the strategy to control antimicrobial resistant (AMR) bacterial infections; one multidrug resistant pathogen of particular concern is Acinetobacter baumannii. In this study we identified two novel A. baumannii antigens using mass spectrometry and phage expression: Oxa23 and P …

Roses are Red
At florists they’re retailed
mRNA can prevent infection
When it is inhaled
#academicvalentines
pubmed.ncbi.nlm.nih.gov/40615441/

Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics - PubMed Two-photon polymerization (2PP) 3D printing enables top-down biomaterial synthesis with nanoscale spatial resolution for de novo design of monodisperse injectable drug delivery systems. Spatiotemporal Controlled Release Inks of Biocompatible polyEsters (SCRIBE) is a novel poly(lactic-co-glycolic aci …

Roses are Red
Violets are Blue
Nanoscale Printable Capsules
Can deliver vaccine when tuned
pubmed.ncbi.nlm.nih.gov/40018867/
#academicvalentines

A sort and sequence approach to dissect heterogeneity of response to a self-amplifying RNA vector in a novel human muscle cell line - PubMed Self-amplifying RNA (saRNA) is an extremely promising platform because it can produce more protein for less RNA. We used a sort and sequence approach to identify host cell factors associated with transgene expression from saRNA; the hypothesis was that cells with different expression levels would ha …

Roses are Red
Violets are Blue
The amount of RNA vaccine in a cell surprised us
It might shock you too
#academicvalentines
pubmed.ncbi.nlm.nih.gov/39759876/

A Transgenic Mouse With a Humanized B-Cell Repertoire Mounts an Antibody Response to Influenza Infection and Vaccination - PubMed The development of a universal influenza vaccine likely requires an understanding of previous exposure to influenza virus (through vaccination or infection) and how that shapes the antibody repertoire to vaccination, sometimes called original antigenic sin or antigenic imprinting. While animal model …

Roses are Red
Violets are Blue
My mate Stevo and I
Tested antibodies against flu
#academicvalentines
pubmed.ncbi.nlm.nih.gov/39317662/

An ultra-early, transient interferon-associated innate immune response associates with protection from SARS-CoV-2 infection despite exposure - PubMed This work was supported by the NIHR Health Protection Research Unit in Respiratory Infections, United Kingdom, NIHR Imperial College London, United Kingdom (Grant number: NIHR200927; AL) in partnership with the UK Health Security Agency and the NIHR Medical Research Council (MRC), United Kingdom (Gr …

Roses are Red
Violets are Blue
An ultra-early, transient interferon-associated innate immune response
Prevents SARS-CoV-2
pubmed.ncbi.nlm.nih.gov/39667271/
(an easy rhyme for starters)
#academicvalentines

In a tradition that literally no one wanted me to continue
Here is this year’s
#academicvalentines a chance to celebrate a year of papers

Science funding needs fixing — but not through chaotic reforms The changes announced by a major UK science funder are putting scientists — and the future of research — in a difficult position.

UK science funding is looking bleak at the moment
www.nature.com/articles/d41...

Deborah Cohen & Prof. John S.Tregoning in conversation | Events at Waterstones Bookshops | Waterstones Events at Waterstones get you closer to the books and authors you admire most. Find information and tickets about Deborah Cohen & Prof. John S.Tregoning in conversation today.

Looking forward to talking about ageing, health and the internet @gowerst-books.bsky.social
www.waterstones.com/events/debor...

Is it true that … you lose most body heat from your head? This 1970s notion is a bit of a myth – but it’s still a good idea to wear a hat if it’s cold out

Myth busted: you don’t lose 40–45% of your body heat through your head. 🧢🌡️

"The head does lose heat, but it’s not uniquely heat-losing."

@prof-tregoning.bsky.social explains you lose heat from any exposed skin – head, face, and legs included! 🥶 www.theguardian.com/lifeandstyle...

Live Forever?: A Curious Scientist’s Guide to Wellness, Ageing and Death Live Forever?: A Curious Scientist’s Guide to Wellness, Ageing and Death : Tregoning, Prof. John S.: Amazon.co.uk: Books

My book launches today in paperback.
It has a bonus chapter on something that actually makes you live longer (and better).

www.amazon.co.uk/dp/1836431082

And don't forget my book Live Forever was in the same list
(not only am a science neighbour in @imperialmed.bsky.social but also am an @imperiallifesci.bsky.social alumni)

Cold plunges and kombucha: Do winter wellness trends work? Experts weigh in on whether cold water swimming, drinking kombucha and taking vitamins can boost your immune system.

Do cold plunges and kombucha really have immune-boosting properties?

Prof. John Tregoning @prof-tregoning.bsky.social says in BBC health, the benefits are mostly indirect and what makes people feel healthier in winter may come down to reducing stress.👇
www.bbc.co.uk/news/article...

Cold plunges and kombucha: Do winter wellness trends work? Experts weigh in on whether cold water swimming, drinking kombucha and taking vitamins can boost your immune system.

7th most read article on the BBC today
www.bbc.co.uk/news/article...

YouTube video by BBC - World at One / PM Why do people want to live forever? | BBC Radio 4 PM

Was on BBC Radio 4 yesterday talking about ageing and people desperately seeking to avoid it!
www.youtube.com/watch?v=KTQ-...

It’s a new term: here are 99 lab hacks Nature asked contributors, editors and working researchers to share their best advice for scientists.

Top lab tips from @nature.com
www.nature.com/articles/d41...

Putin’s quest for longevity – podcast At a recent ceremony for world leaders in Beijing, a hot mic picked up a surprising exchange between Vladimir Putin and Xi Jinping about the possibility of living to 150. Putin suggested the secret mi...

Putin, Xi and the obsession with radical life extension. Fascinating chat with @prof-tregoning.bsky.social and @pjotrsauer.bsky.social 🧪
With @elliebury.bsky.social
And Tom Glasser
www.theguardian.com/science/audi...

Immunology of RNA-based vaccines: the critical interplay between inflammation and expression Given the explosion of RNA vaccines in the last 5 years, this review explores gaps in knowledge and what is needed to fill these. Tregoning and colleagues focus on the immune response to RNA vaccines,...

Great to see our review about RNA vaccines out.
It covers the knowns and unknowns and where next

www.cell.com/molecular-th...

Don't forget, you can get another 'dose' of @prof-tregoning.bsky.social next week at WeHaveWaysPod WHWFest ... there's more to John than meets the eye! Author of Live Forever?, he also played the drums (IYKNK) - and knows his stuf, WW2-wise ...

Jolly excited doesn't begin to cover it! From Moran to Morell, with infections, gangrene (😷), immunology and all things medical in between - our guest editor this week on #NewsoftheWar is @prof-tregoning.bsky.social ...

linktr.ee/NewsOfTheWar

With @prof-tregoning.bsky.social as our special guest editor this week, we have hard-hitting facts - and EXCELLENT RABBIT HOLES - about gangrene, penicillin, mortality, immunology, and more. @andyaitch.bsky.social @merrynwalters.uk

Jolly excited about this weeks episode, in a mid season break of 1941 we have our first guest editor for News Of The War!

@prof-tregoning.bsky.social joins @andyaitch.bsky.social and @merrynwalters.uk to talk about infections, immunology, horse serum and much more.
🚨 Friday 5th September 🚨

💉🌐 Oral vaccines are cheap, easy & life-saving, but why do they underperform in the places they’re needed most?

This paper highlights multifactorial causes & urges tailored solutions for equity in immunisation.

👉 https://bit.ly/42aACy3

#ImmunisationAwarenessMonth

Manipulating the delivery and immunogenicity of DNA vaccines through the addition of CB[8] to cationic polymers Nucleic acid vaccines require antigen expression and inflammation to induce the adaptive immune response. Sallah and colleagues explored the use of cucurbiturils as supramolecular “hosts” to crosslink...

And third, manipulating DNA delivery through the inclusion of cucurbit[n]urils (CBs), as supramolecular “hosts,” into nucleic acid-polymer polyplexes
www.cell.com/molecular-th...

Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics - PubMed Two-photon polymerization (2PP) 3D printing enables top-down biomaterial synthesis with nanoscale spatial resolution for de novo design of monodisperse injectable drug delivery systems. Spatiotemporal...

Secondly in collaboration with @djpeeler.bsky.social
David developed a novel long lasting drug delivery approach using teeny tiny particles printed on a tiny 3D printer
pubmed.ncbi.nlm.nih.gov/40018867/

Intranasal delivery of mRNA expressing newly identified Acinetobacter baumannii antigens protects against bacterial lung disease - npj Vaccines npj Vaccines - Intranasal delivery of mRNA expressing newly identified Acinetobacter baumannii antigens protects against bacterial lung disease

Just to flag some papers from the lab
First up - we identified novel antigens from Acinetobacter baumannii and demonstrated greater protection when delivered intranasally
www.nature.com/articles/s41...

Very excited that we are looking for a new lab member. If you are interested in viral immunology have a look at this job advert. You will work in 2 labs, ours and @prof-tregoning.bsky.social at @imperialnhli.bsky.social @imperialinfect.bsky.social