@tim_stasevich

Where Do Colorado Officials Stand on Proposed ICE Facility in Hudson? Several members of Colorado’s congressional delegation sent a letter to Department of Homeland Security and ICE leadership Tuesday opposing a proposed ICE detention center in Weld County. Rep. Brittan...

heard from @projectsaltbox.bsky.social that #ICE is planning a Trump concentration camp in Hudson CO. Does anyone have intel? Protests happening? Our Dem. lawmakers at least are opposed. coloradotimesrecorder.com/2026/02/wher...
and y'all the tracker on @projectsaltbox.bsky.social is awesome.

Intelligent Imaging Innovations - Microscopist Intelligent Imaging Innovations

3i is hiring a Microscopist - repost & tell a friend!
Ideal candidate has experience in fundamental optical theory & familiarity with lightsheet, spinning disk, multiphoton, TIRF etc. Full-time, exempt position (travel to visit customers) offering a competitive salary & comprehensive benefits.

Max Fels @mfels.bsky.social from our lab discovers giant DNA viruses that infect amoeba encode eIF4E and the entire suite of 4F complex proteins to control mRNA translation, including beautiful crystal structures of viral 4E bound to modified mRNA 5' caps:

www.cell.com/cell/fulltex...

GitHub - imaging-formats/bffile: Modern Bio-Formats wrapper with clean lazy Python API Modern Bio-Formats wrapper with clean lazy Python API - imaging-formats/bffile

Rewrote my Python bioformats wrapper (from aicsimageio/bioio) as a standalone package:

- fully bootstrapped Java setup (just pip install)
- lazy, repeatably-indexable Array object
- fully spec-compliant OME-Zarr group obj.
- xarray/dask exports

github.com/imaging-form...
v0.0.rc1 on PyPI

Highlights from Japan A selection of research from scientists based in institutions in Japan.

Our collection presents a selection of research from scientists based in Japan.

Our model is designed to make publishing faster, fairer and more transparent, and this collection showcases research with global scientific contributions.
buff.ly/mUQxLxL

We are very proud that Thalia has been nominated for three Yorkshire Choice awards - Please vote here: www.yorkshirechoiceawards.co.uk/vote

Three years ago, Thalia was diagnosed with a devastating brain tumour. She has fought back and done her best to raise awareness and funds to support others!

Love this. Congrats.

Our new paper's out: FidlTrack—structure-aware single-particle tracking benchmarks/boost SPT fidelity

With it we resolve with sub-organelle res. e.g. BACE1 amyloidogenic APP cleavage #Alzheimers, ER exit events, map nanobody binding in realtime in ER/organelles 🔬🧠#SingleMolecule

rdcu.be/e3Ris

Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity In this study, an imaging technology, “VISUN,” is developed that allows live-cell visualization of unmodified influenza virus during infection with single-vRNP resolution. Combining VISUN with multipl...

Out now in Cell Systems: We develop a technique to visualize influenza A virus in live cells with single viral RNA resolution. Combined with precise readouts of viral transcription, we map the whole influenza A viral life cycle and identify key bottlenecks in infection.

www.cell.com/cell-systems...

Beautiful work!

Single-molecule dynamics of the TRiC chaperonin system in vivo - Nature Single-particle tracking experiments in intact cells reveal dynamic co- and post-translational interactions of the TRiC–PFD chaperonin complex with client proteins during in vivo protein folding.

I’m excited to share my first-author paper, with co-first author @rongqinxiaoxiao.bsky.social, now out in @nature.com. We developed a live-cell single-particle tracking platform to see how TRiC & prefoldin engage proteins during co- and post-translational folding. 1/9 www.nature.com/articles/s41...

A high-resolution, easy-to-build light-sheet microscope for subcellular imaging An accessible light-sheet microscope delivers subcellular-resolution, multicolor volumetric, and live-cell imaging, lowering barriers to state-of-the-art performance.

In @elife.bsky.social: A high-resolution, easy-to-build light-sheet microscope for subcellular imaging doi.org/10.7554/eLif...

Live-cell single-molecule dynamics of eukaryotic RNA polymerase machineries Eukaryotic gene expression is orchestrated by RNA polymerases (RNAPI, II, and III) and associated factors, yet their real-time dynamics remain obscure. Using single-molecule tracking in living yeast, ...

🧬🔬🎥 @science.org Live-cell single-molecule dynamics of eukaryotic RNA polymerase machineries | Science www.science.org/doi/10.1126/...

What nanobodies can do for you - Nature Methods Since the chance discovery of nanobodies in the late 1980s, their uses and applications have kept growing. Researchers are now exploring new ways to harness nanobody versatility.

Researchers are exploring new ways to harness the versatility of nanobodies, which are smaller camelid antibodies. @vivienm.bsky.social asked scientists about some of their current and future nanobody work.

www.nature.com/articles/s41...
and rdcu.be/e1yTd

New preprint from the lab! We identify the ZnF protein Mulberry as a condensation-dependent structural regulator of genome topology that organizes “multi-way regulatory hubs” in early Drosophila embryos.
www.biorxiv.org/content/10.6...

Congratulations!!

This was a great chance to work with @IwasakiLabRiken to discuss the nearly perfect complementarity of Ribo-Seq and single molecule imaging studies of mRNA translation. I hope there are more studies in the future combining these two modalities to unravel translational regulation.

Yesterday, five-year-old Liam and his dad Adrian were released from Dilley detention center. I picked them up last night and escorted them back to Minnesota this morning.

Liam is now home. With his hat and his backpack.

Notice of Temporary Extension of Eligibility for the NIH Pathway to Independence Award (K99/R00):
for Individuals whose final due date was September-November 2025 and January-March 2026. As always, reach out to your PO/IC contact to discuss.
grants.nih.gov/grants/guide...

Does the noncoding genome actually carry more genetic information than coding seqs? Motivated by this question we mutated every bp in the 10kb MYC locus. Results are even more exciting: Decoding the MYC locus reveals a druggable ultraconserved RNA element www.biorxiv.org/content/10.6...

Developability comes for free...? Did AI de novo antibody generation models learn developability properties without explicitly being trained on this?

Love this theory for why antibody generative models produce sequences with nice developability properties: it’s a reflection of PDB. ayusuf.substack.com/p/developabi....

This is a very nice example of one way of thinking about conditional generative models.

Seeing Chromatin Scaling with Cell Size, from Interphase to Mitosis - FocalPlane Seeing Chromatin Scaling with Cell Size, from Interphase to Mitosis -

Melike Lakadamyali @melikel.bsky.social, Jérôme Solon and Manuel Mendoza @longchrom.bsky.social share the behind the paper story of their research interrogating how chromosome compaction scales with cell size using a combination of live confocal microscopy and 3D STORM.

Pre-assembly of biomolecular condensate seeds drives RSV replication Nature - Viral ribonucleoprotein–viral protein networks form pre-replication centres that nucleate viral factories and drive respiratory syncytial virus replication.

Now out in Nature! We visualize infection of the RNA virus RSV in real-time with single-vRNP resolution to understand how RSV establishes viral factories, biomolecular condensates that act as sites of viral replication. A huge collaborative effort led by Dhanushika Ratnayake!

rdcu.be/e1bBW

i have just gotten off a productive call with sauron where i laid out our requests

- nazgul bodycams
- morgul knife must remain sheathed unless suspect is determined to be carrying the one ring
- shelob will be the new point of contact

General strike now.

AG Bondi demands access to Minnesota voter rolls after fatal Border Patrol shooting Her demand came as Minneapolis was reeling from a second fatal shooting by a federal agent this month.

If you want proof that Trump is going to try to rig the elections, here it is:

In a letter to Gov. Tim Walz Saturday, Bondi claimed that Walz could “restore the rule of law” by complying with a list of demands, including giving the Department of Justice (DOJ) the state’s voter registration records.

‘This is what fascism looks like’: terror in Minneapolis reminiscent of civil war Alex Pretti’s death could be a moment of reckoning for Democrats to call time on Trump waging war on his people

‘This is what fascism looks like’: terror in Minneapolis reminiscent of civil war

There's an interesting parallel between the in silico affinity maturation presented in the PPIFlow paper, which turns the antibody design problem into a motif scaffolding problem, and what the adaptive immune system does with the so-called germline-encoded GRAB motifs www.biorxiv.org/content/10.6...

Potential fork of PPIFlow with a more permissive license is very exciting: github.com/Mingchenchen...
Highly recommend their paper: results are super impressive and it's *not* an AF3 clone

First preprint of the year! New work from @jimmy-ly.bsky.social revealing unexpected roles for 5' UTR length in controlling alternate translational isoforms - important implications for both physiological cell function and rare disease. Small changes -> big impacts.

www.biorxiv.org/content/10.6...