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Ryan Abdella

@tinyfrozenproteins.bsky.social

Research Assistant Professor and CryoEM Facility Manager at the University of Minnesota interested in transcription, viral replication, and host-pathogen interactions.

379 Followers  |  129 Following  |  8 Posts  |  Joined: 26.09.2023  |  1.4264

Latest posts by tinyfrozenproteins.bsky.social on Bluesky

Post image 17.11.2024 23:54 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0

I should add this is being processed in Cryosparc ATM. I’ll try 3D classification after and hope that can clear up some of the misaligned particles but would be nice to be able to select on metadata.

15.11.2024 16:12 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

#cryoEM folks, what’s your favorite trick for focused refinements? I have ~4 million symmetry expanded particles and it’s clear some will wander away too far from their correct alignment. Is there a way to select particles based on magnitude of alignment changes?

15.11.2024 16:09 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 3    πŸ“Œ 0

Hi everyone! I’m a postdoc at the University of Minnesota working on viral replication and host-pathogen interactions.

15.11.2024 12:34 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

I would like to be added please!

15.11.2024 12:29 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0

Awesome work! Have you or others attempted to put any of the disulfides into a NabFab?

14.06.2024 14:16 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

#cryoEM folks, why are facilities forgoing globus in favor of physical hard disks? - sincerely, impatient postdoc

10.10.2023 03:23 β€” πŸ‘ 3    πŸ” 1    πŸ’¬ 1    πŸ“Œ 0

Native CTD reading when?

29.09.2023 15:51 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0

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