Lisanne Mout

Optimization of AsCas12a for combinatorial genetic screens in human cells - Nature Biotechnology Improved Cas12a variants and sgRNA design rules enhance genome-wide screens.

This looks really cool! Would you consider including variable direct repeats, this should minimize homology between the interspersed repeats? >>> doi.org/10.1038/s415...

1/ You run ChIP-seq to find where your transcription factor binds. You run RNA-seq to see which genes change expression. Now, you ask a "simple" question, what are the "direct targets"?

GitHub - RILAB/statements: Successful Job Applications and Grants Successful Job Applications and Grants. Contribute to RILAB/statements development by creating an account on GitHub.

Lunch today halfway around the world in Hangzhou and met a new asst professor who was very thankful for this resource. Please help out folks like them and other early career scientists with examples of job apps. Submit yours!
github.com/RILAB/statem...

Fluctuating DNA methylation tracks cancer evolution at clinical scale - Nature Cancer evolutionary dynamics are quantitatively inferred using a method, EVOFLUx, applied to fluctuating DNA methylation.

Studying cancer evolution needs multi-region or single cell seq for phylogenetics, right? Amazingly (I think!) we found single-sample bulk methylation suffices, via analysis of "fluctuating methylation". In @nature.com today led by brilliant @calumgabbutt.bsky.social www.nature.com/articles/s41...

8 Resources to study Transcription factor binding, enhancers and histone modification distribution
 1. ENCODE www.encodeproject.org/

Included in this is SickKids in Toronto, by the way. So Canadian kids with cancer will also be affected by this.

Imagine voting for kids with brain tumors to lose access to clinical trials.

“‘She uncovered the social experience of women in science’ — the lab assistants who never became managers, the geologists poring over data in government offices while their male peers were doing fieldwork, those who despite their advanced degrees didn’t get hired or promoted, or who were sidelined…”

Two PhD positions available in our lab to study #chromatin, #epigenetics and cancer

NASP modulates histone turnover to drive PARP inhibitor resistance - Nature PARP inhibitor treatment triggers histone release from the chromatin in cancer cells; consequently, targeting the histone chaperone NASP renders cells vulnerable to PARP inhibition.

Thrilled to share our latest work, just published in @nature.com ⬇
www.nature.com/articles/s41...

We discovered that PARP inhibitors 💊 trigger histone eviction from the chromatin and this creates a hidden vulnerability in PARPi resistant tumors.
🧵 (1/8)

Image credit: @gloglita.bsky.social‬ @lifescienceeditors.bsky.social‬ captured DynaTag in action: a pA-Tn5 probe (multicoloured) binds an antibody (white), which binds p53 DNA-binding domain (green) on DNA (blue) within 2 nucleosomes

🧪Move over CUT&Tag, there’s a new #TranscriptionFactor mapping method in town.
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)

Please pread the word.

We have just launched the Hubrecht International PhD Program (HIPP).

Are you looking for a PhD position in molecular and developmental biology or related subject in an international, very supportive and collaborative environment?

Then apply to the HIPP!! 👇

Range extender mediates long-distance enhancer activity - Nature The REX element is associated with long-range enhancer–promoter interactions.

Our paper describing the Range Extender element which is required and sufficient for long-range enhancer activation at the Shh locus is now available at @nature.com. Congrats to @gracebower.bsky.social who led the study. Below is a brief summary of the main findings www.nature.com/articles/s41... 1/

Transcriptional and post-transcriptional regulation of transposable elements and their roles in development and disease - Nature Reviews Molecular Cell Biology Transposable elements (TEs) comprise nearly half of the human genome. This Review discusses transcriptional and post-transcriptional mechanisms that repress TE activity, how TEs escape this suppressio...

We wrote a review on Transposable Elements (TEs) and almost all aspects of TE silencing and their roles in biological processes & disease.
www.nature.com/articles/s41...

1/ Working with big data in R?
Your wrangling just got a massive upgrade.
duckplyr is now in the tidyverse—and it’s fast. Really fast. 🧵

Applications are now open! We are recruiting 20 Assistant Professors in a wide range of subject areas. We're looking for early-career researchers with strong scientific merits and future potential.
🔗 All positions: ki.se/en/about-ki/...

Postdoctoral Associate – Nima Sharifi Laboratory - Miami, Florida (US) job with University of Miami Desai Sethi Urology Institute | 12840300 The Nima Sharifi Laboratory is seeking to recruit outstanding postdoctoral associates with a primary interest in the fundamental mechanisms of huma...

Do you have a primary interest in fundamental mechanisms of human biology, biochemistry, metabolism, genetics, or medicinal and analytical chemistry and looking for a postdoctoral fellowship? 🧪 www.nature.com/naturecareer...

@saramostafavi.bsky.social (@Genentech) & I (@Stanford) r excited to announce co-advised postdoc positions for candidates with deep expertise in ML for bio (especially sequence to function models, causal perturbational models & single cell models). See details below. Pls RT 1/

Are you @ the late stages of your postdoc ? Want to pursue a PI career ?

Then this 👇👇 is for you ! Apply for a spot @ our Talent Forum
www.helmholtz-munich.de/en/stem-cell...

Peer-networking / Career orientation and more !!!

Please distribute :) 🙂

How a discovery in Yellowstone National Park led to the development of PCR - Richmond Scientific A discovery in Yellowstone National Park led to the development of PCR, the gold-standard COVID-19 tests used to fight the global pandemic.

Reminder: Nobel-prize winning PCR (1983), used in basically all genetic tech today, was only possible because of extremophile bacterium discovered in 1964 in Yellowstone funded by a small ~$80k NSF grant with no obvious application at the time. #science 🧪
www.richmondscientific.com/how-a-discov...

Assistant Professor Computational Analysis of Single-cell and Spatial Omics Data Are you passionate about developing omics data analysis methodology? Are you fascinated by the opportunities of omics data at single-cell or spatial resolution? Would you like to work at the forefront...

Job opportunity at our institute at the University of Amsterdam:
www.academictransfer.com/en/jobs/3519...

Endometrial tumorigenesis involves epigenetic plasticity demarcating non-coding somatic mutations and 3D-genome alterations - PubMed In summary, we identify a complex genomic-epigenomic interplay in EC development and progression, altering 3D genome organization to enhance expression of the critical driver ERα.

Check out our latest paper! Out now in Genome Biology! Many thanks to all coauthors for their invaluable contributions on this lengthy journey pubmed.ncbi.nlm.nih.gov/40346709/

Circular RNA in aggressive tumours

Abundant circRMST molecules were found in aggressive prostate and lung cancers, interacting with transcription factors SOX2 & NKX2-1 to drive oncogene ASCL1 expression, reported by @pmresearch-uhn.bsky.social & @danafarber.bsky.social

www.cell.com/cancer-cell/...

She Worked in a Harvard Lab to Reverse Aging, Until ICE Jailed Her (Gift Article) President Trump’s immigration crackdown ensnared Kseniia Petrova, a scientist who fled Russia after protesting its invasion of Ukraine. She fears arrest if she is deported there.

The Americans are doing to Kseniia what the Russians did to Brittney Griner for having 0.7 grams of cannabis oil. Except no one is coming to negotiate her freedom and Harvard is sitting on their hands. I am so ashamed. Please share this story.
www.nytimes.com/2025/04/11/s...

1/ As a biologist, you work with a lot of data. But do you know how to use it to its fullest? Learning to program can change your research and career! Let's explore the top tips to get you started.

The landscape of N6-methyladenosine in localized primary prostate cancer - Nature Genetics Transcriptome-wide m6A RNA methylation profile in 162 primary prostate tumors identifies m6A association with prognostic clinical features and disease aggression.

The latest research from the He Lab (www.hansenhelab.org) reinforces the importance of integrating epigenomic assays into clinical practice to enhance cancer patient care. Congratulations to the entire team!
@uhnresearch.bsky.social @pmresearch-uhn.bsky.social
doi.org/10.1038/s415...

The end of the genetic paradigm of cancer journals.plos.org/plosbiology...

Whole genome sequencing of 378 prostate cancer metastases reveals tissue selectivity for mismatch deficiency with potential therapeutic implications - PubMed NCT01855477.

Check out our latest paper! WGS on 378 metastases of patients with prostate cancer. Biggest findings: 1. Non-coding mutations converge on DNA damage repair 2. Coding mutations defer between metastatic sites, with potential implications on systemic therapy selection.
pubmed.ncbi.nlm.nih.gov/40114169/

postdoc opportunity - join our team! · Verhaak Lab The Verhaak lab in the Department of Neurosurgery at the Yale School of Medicine is looking to recruit a postdoctoral associate who will help us develop new therapeutic approaches to treat patients wi...

We are looking to recruit new postdocs! Please check verhaaklab.com/archive/2025....

Here's the entry form: ow.ly/80eS50Vey4X

Postdoctoral Fellow (Bioinformatics & Computational Biology) - Parkville, Melbourne (AU) job with Peter MacCallum Cancer Centre – Parkville | 12837684 Applicants are invited to join an exciting multi-discliplinary research program within Mark Dawson’s Laboratory at the Peter MacCallum Cancer Centre.

We are looking for a talented postdoctoral bioinformatician @thedawsonlab.bsky.social to work with us on single cell multiomics incorporating lineage tracing and spatial technologies- we specifically want someone curious, fearless with a licence to innovate!! Come join us!!