Ruizhao WANG

PANCS-spec-Binders: A system for rapidly discovering isoform- or epitope-specific binders Proteins that bind to a target protein of interest, termed "binders," are essential components of biological research reagents and therapeutics. Target proteins present multiple binding surfaces with ...

Check out our newest work! This is a story on how to get selectivity in binders - both isoform and site selectivity. Read the paper or enjoy this brief Skytorial of what we did!

www.biorxiv.org/content/10.1...

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How cool it is!!

How do cells adapt morphology to function? In a 🔥 preprint by @zjmaggiexu.bsky.social , with @dudinlab.bsky.social and @amyweeks.bsky.social , we identify a self-organizing single-cell morphology circuit that optimizes the feeding trap structure of the suctorian P. collini. 🧵 tinyurl.com/4k8nv926

A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording - Nature Biotechnology Prime editing in mammalian cells benefits from a comprehensive list of genetic parts.

A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording - @jshendure.bsky.social @troymcdiarmid.bsky.social @uwgenome.bsky.social go.nature.com/49eTPCu

Fun to see this thread answer some questions I had, thank you.

Cool! This could have a lot of influence on sensors, pull down, signaling rewiring...🤔

I see👍 thank you!

I would like to hear some suggestions on why you think we should not use it?

🚀 Our new paper is out @natmethods.nature.com!

Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.

🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/

Thank you, looks very cool! Can I message you about the cell marker you are using here?

Very cool! What kind of cell types are used here, and what kind of microscope is used? How long does the imaging take?

PANCS-Binders (phage-assisted noncontinuous selection of protein binders) screens multiple high-diversity protein libraries against a panel of dozens of targets for high-throughput binder discovery. @chembiobryan.bsky.social @mstyles-chembiol.bsky.social

www.nature.com/articles/s41...

That was so cool!

Humanized Caffeine-Inducible Systems for Controlling Cellular Functions https://www.biorxiv.org/content/10.1101/2025.06.13.659463v1

That was cool👍

Cool, intresting findings, could be invented as tool for transcription control?

Very cool study!

I have a lot of recommendations if you are in China😀

Genomes encode biological complexity, which is determined by combinations of DNA mutations across millions of bases

In new work @arcinstitute.org, we report the discovery and engineering of the first programmable DNA recombinases capable of megabase-scale human genome rearrangement

Congrats! Useful tools!

Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks - Nature Biotechnology Far-red kinase biosensors are applied to image kinase signaling networks at super resolution.

Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks - @michellefrei17.bsky.social @jinzhanglab.bsky.social go.nature.com/3GfqrzL

Smart idea! Bring phos-sensor to transcription activation?

Wow, I heard the report before. Congratulations!

Wow, congratulations!

Engineered nucleocytosolic vehicles for loading of programmable editors Engineered nucleocytosolic vehicles for loading of programmable editors (ENVLPE) with enhanced CRISPR ribonucleoprotein (RNP) loading and stabilization enable the efficient delivery of gene-editing ef...

www.cell.com/cell/fulltex...
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @cellcellpress.bsky.social. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models. All core constructs are available from @addgene.bsky.social.

Great work and very impressive!

Haha

Wow, congratulations!

Congrats!!

LIDAR is out! rdcu.be/efugD We are one baby step closer to making it useful for therapeutics. NIH high-risk high-reward program provides key support.