Nanodiamonds for spatial resolution benchmarking in two-photon microscopy Reliable and reproducible measurement of spatial resolution is essential for validating and comparing the performance of two-photon microscopy systems. We present fluorescent nanodiamonds as robust, photostable, reusable, and biocompatible probes for benchmarking spatial resolution. Owing to their nanoscale dimensions and stable fluorescence, nanodiamonds act as near-ideal point-like emitters, enabling accurate characterisation of the point spread function across varying imaging conditions. We demonstrate that nanodiamond-based phantoms serve as a reliable alternative to conventional fluorescent beads embedded in agarose, while at the same time offering advantages in stability and optical properties. Our results position nanodiamond phantoms as a next-generation calibration material that bridges ease of use and reproducibility, advancing quantitative imaging and cross-platform comparability in modern fluorescence microscopy. ### Competing Interest Statement The authors have declared no competing interest. Wellcome Trust, https://ror.org/029chgv08, Investigator Award in Science 220277/Z20/Z European Research Council, https://ror.org/0472cxd90, ERC-StG “NeuroVisEco” 677687, ERC AdG “Cones4Action” covered under the UK’s EPSRC guarantee scheme EP/Z533981/1 UK Research and Innovation, https://ror.org/001aqnf71, BBSRC, BB/R014817/1, BBSRC, BB/W013509/1 International Human Frontier Science Program Organization, https://ror.org/02ebx7v45, RGP001/2025 Leverhulme Trust, https://ror.org/012mzw131, PLP-2017-005, RPG-2021-026, RPG-2-23-042 Lister Institute of Preventive Medicine Gdańsk University of Technology, https://ror.org/006x4sc24, Nobelium DEC-4/1/2024/IDUB/I.1a/No, Platinum 1/1/2025/IDUB/I.1B/Pt

1/10 New preprint on bioRxiv
Nanodiamonds for spatial resolution benchmarking in two-photon microscopy

We introduce nanodiamond-based phantoms as a robust, reusable alternative to bead–agarose samples for PSF calibration.

www.biorxiv.org/content/10.6...

14.01.2026 15:28 — 👍 6    🔁 5    💬 1    📌 0

💎 New Publication Alert: Next-Generation Calibration Materials
We propose fluorescent nanodiamonds as a superior alternative to standard beads for calibrating two-photon systems.
Check out: www.biorxiv.org/content/10.6...
#Microscopy #Science #Innovation #Photonics #AcademicPublishing

14.01.2026 13:07 — 👍 1    🔁 1    💬 0    📌 0

Polymer Materials Characterization For U-Shape Optic Fiber Sensors: A Review - [v1]

If someone is interested in fiber optic then I reccomend our review (preprint) : www.preprints.org/manuscript/2...

09.12.2024 17:04 — 👍 0    🔁 0    💬 0    📌 0