Anja Schmidt

A photo of a coffee mug. The mug is labeled with writing that says "hhmi | janelia Research Campus." The drink in the mug has the words "Insight From Imaging 2025" printed on the foam.

One last Bob's coffee break at @hhmijanelia.bsky.social before our final #InsightFromImaging activity: Participants are working in groups to formulate a hypothesis and design an imaging experiment to test it 🔬

For our final lecture at #InsightFromImaging 2025, @jesseaic.bsky.social is discussing electron microscopy and correlative approaches, including results from our FIB-SEM system, which can achieve 4 nm resolution in 3D!

A 3D rendering of microscopy data. Fluorescently labeled tubulin is shown in green, while second harmonic generation is shown in magenta.

We spent the afternoon at #InsightFromImaging in the lab exploring 5 different microscopes (& 5 different samples!) to understand approaches for imaging depth.

Kidney slice prep by Ellen, imaged by Jenny using 2P (green tubulin) + SHG (magenta), viz by @scientistrachel.bsky.social in #AGAVE

A microscopy image with text annotation. The left side of the image is labeled "System Correction Only" and shows blurry and aberrated mitochondria. The right side of the image is labeled "Adaptive Optics + Deconvolution" and shows a clearer image of mitochondria.

Today at #InsightFromImaging we're focusing on imaging depth, starting with a lecture by @cmhobson.bsky.social. Adaptive optics (available on our MOSAIC system!) can improve deep imaging, as shown by these mitochondria in a zebrafish embryo.

A collage of photos, which show people analyzing images and giving presentations.

This morning at #InsightFromImaging we focused on particle tracking. After a lecture by @jesseaic.bsky.social, participants split into groups to use #TrackMate to track our HL60 migration data from yesterday, followed by presentations on their results 📈

HL60 cells take on so many fun shapes as they migrate! This #InsightFromImaging data features cells prepared by Leanna and imaged on the @aicjanelia.bsky.social LLSM by @cmhobson.bsky.social

Continuing our speed theme at #InsightFromImaging, we spent the afternoon at the microscope exploring imaging speed, as well as tradeoffs in imaging scale. Video featuring imaging of neutrophil-like HL60 cells on a widefield vs the AIC's LLSM.

A photo of a classroom. A man stands at the podium next to a projector screen. The audience sits at tables in front of the screen.

Today's theme at #InsightFromImaging 2025 is imaging speed. We're kicking things off with a lecture by Chad Hobson.

A microscopy image of a cell labeled for actin (magenta) and paxillin (green). The left half of the image is taken in widefield mode, while the right half is taken in confocal mode. The scale bar is 10 um.

For our first #InsightFromImaging lab, we're exploring imaging resolution across a variety of microscopy systems. When is increased resolution really necessary? What trade-offs does it require?

A photo of a classroom. There is a projector screen at the front of the room, and participants sit at tables with laptops performing image analysis tasks.

This morning at #InsightFromImaging we jumped into hands on image analysis activities, including comparing the pros and cons of imaging denoising approaches

A photo of a man giving a presentation. He is standing in front of a projector screen. In the front of the photo, the audience sits at long tables.

We're kicking off #InsightFromImaging 2025 with a keynote lecture by @scopeshifu.bsky.social on advanced microscopy and the resources available through the Advanced Imaging Center's visitor program 🔬

A 3D rendering of microscopy data. The structure of a fish is visible in gray while green blood vessels are visible through the eye.

When #FluorescenceFriday falls on Halloween, we break out the spooky data 👻 Here we are looking at the blood vessels of a zebrafish through its eye 🎃

Wishing everyone a happy #FluorescenceFriday and a #HappyHalloween! This expansion microscopy visualization of blood vessels in a zebrafish is the result of a collaboration between Ellen, Jenny, @nicolasdenans.bsky.social, and @scientistrachel.bsky.social.

A microscopy image of a cell stained for actin (magneta color) and focal adhesions (green color).

Our team is hard at work preparing for #InsightFromImaging 2025! We can't wait to welcome HHMI #HannaGrayFellows to @hhmijanelia.bsky.social next week for lectures, hands-on labs, and activities focused on designing informative and quantitative bioimaging experiments 🔬

That was challenging and big fun! Safe travels home!

A photo of a group of people standing in front of scientific equipment.

Today we're wrapping up 3 weeks of zebrafish imaging on our MOSAIC system. We've enjoyed having Lisa and Miray of @annamolofskylab.bsky.social here and wish them safe travels home!

The WB-ExM protocol described here works with.... every sample we tested! Here a 3do quail embryo (white= pan-protein; red=MF20) www.biorxiv.org/content/10.1...

Another three imaging-packed weeks :) it was a pleasure working with you.

My first SiMView project and it was such a fun one. Especially working with my favorite organism :) there is never anything wrong about staring at developing Drosophila in 4D!

Our deadline is coming up soon :)

Less than one month left to apply for our current call for proposals (deadline June 16)! Reach out to our team for a free technical consultation before proposal submission. Learn more at www.aicjanelia.org/apply 🔬

A photo of a group of people in a laboratory setting. The four people are standing on the right side of the image in front of a microscopy set up.

Today we are wrapping up a MOSAIC project imaging Drosophila with @blankenshiplab.bsky.social Wishing them safe travel home and happy data analyzing!

We’ve had an incredible experience being hosted at the AIC at Janelia for iPALM imaging. Tremendous support and expertise.
If your project could benefit from advanced imaging, check out their instruments (FIB-SEM, iPALM, LLSM and others) and get in touch.👇

A new call for proposals is open at our Advanced Imaging Center for imaging on our pre-commercial microscopes. Reach out for a technical consultation and learn how to apply to use the newest technology for free!

Research Technician - Open Chemistry Primary Work Address: 19700 Helix Drive, Ashburn, VA, 20147 Current HHMI Employees, click here to apply via your Workday account. Summary: Open Chemistry is a collaborative research group whose missio...

We're looking for 1-2 enthusiastic early career chemists to join our Open Chemistry team at @hhmijanelia.bsky.social. Come help us create amazing dyes and share them with the world. Please share with the best recent/soon-to-be B.S. chemists you know! bit.ly/42w8C6N

That was some fun imaging!

Come and work with us. We're really fun :)

Microscopy Training Grants Supporting microscopy training for early-career researchers in cell biology Apply for a grant of up to £1,000 Application dates: 7 March 2025, 6 June 2025, 5 September 2025, 7 November 2025 Online of a person looking down a microscopy with a computer next to them. Find out more at biologists.com/grants/jcs-focalplane-training-grants QR code Journal of Cell Science logo, FocalPlane logo

We’ll be continuing to award JCS-FocalPlane Training Grants in 2025. They are open to ECRs that otherwise wouldn’t have sufficient funding to attend a microscopy or bioimage analysis course to support their research in cell biology.
www.biologists.com/grants/jcs-f...
@jcellsci.bsky.social

YouTube video by amiableamy24 The West Wing-Pluie

This was exactly my first thought! youtu.be/Avo0-8GvBlA?...

A photo of a group of people in front of a festive background. The front row of people are kneeling and the people are holding various photo props such as funny glasses.

Many thanks to the @hhmijanelia.bsky.social Lab Coordinators for another wonderful Cookie Party! The @aicjanelia.bsky.social / @i2janelia.bsky.social team had a ton of fun!

Photo featuring: @desantis42.bsky.social, @mairahmoon.bsky.social, @harikrushnan.bsky.social, @anjanja1024.bsky.social