Jens Schmidt

12 ms exposure 250 fps doesn’t math.

Its technically not the correct term. B is simply lethal when A is not around. Text book example would be B creates a toxic metabolite that A gets rid of.

Sounds like isoform B is dominant negative lethal in the absence of isoform A.

16 posts??? TLDR 🤣

Global inhibition of deadenylation stabilizes the transcriptome in mitotic cells In the presence of cell division errors, mammalian cells can pause in mitosis for tens of hours with little to no transcription, while still requiring continued translation for viability. These unique...

New preprint! We solve a mystery you didn't know existed. Mitotic cells lack new transcription but require ongoing translation. Interphase mRNA half life is only 2-4 hrs. So how do cells arrest in mitosis for hours without depleting their transcriptomes?

www.biorxiv.org/content/10.1...

Dynamic assemblies and coordinated reactions of non-homologous end joining - Nature Structures associated with the final steps of non-homologous end joining (NHEJ) and gap filling present new targets for NHEJ inhibition to enhance efficacy of radiotherapy and accuracy of gene editing...

Excited this study is out - we helped with an experiment on Paxx www.nature.com/articles/s41...

New pre-print! Ryan Kim et al distinguish the roles of the Spindle Assembly Checkpoint as a surveillance pathway only triggered by mitotic errors vs a mitotic timer that is required in every cell division.
www.biorxiv.org/content/10.1...

The 3rd Berlin Single Molecule Biophysics Course will run from 8th to 12th September at the HU-Berlin, Institute of Biology.
Learn about single channels, single molecule TIRF, theory and analysis. Apply by 7th July at bsmbc@icloud.com. Fee waivers and bursaries available.

! Please share widely !

Are you looking for a faculty job? We are hiring in my department at Roswell Park NOW. Contact me for details as I await the formal ad. (Please RT.)

‘The first experiments produced just jaw-dropping results’ Stanford neuro-oncologist Michelle Monje is pursuing a cure for a deadly pediatric brain cancer – and reshaping our understanding of how cancer and brain development intersect.

🧪
#neurology
#neuroskyence
tinyurl.com/bp5d8386
Another beautiful example of NIH-SPONSORED bedside to bench and back to bedside work from an outstanding clinician scientist. This work is producing some of the most compelling responses to treatment in what is arguably the worst pediatric brain tumor.

First you have to understand what that sentence means???

53BP1/RIF1 and DNA-PKcs show distinct genetic interactions with diverse chromosomal break repair outcomes. DNA double strand breaks (DSBs) are the effective lesion of cancer radiotherapy and induce gene editing. 53BP1 accumulates at DSBs and is implicated in end joining (EJ) repair, but its influence on DS...

New preprint from the lab @kaelamakins.bsky.social Genetic interactions between 53BP1-RIF1 and DNA-PKcs for DSB repair outcomes. www.biorxiv.org/content/10.1...

A great end to another Telomeres CSH meeting! It was nice to see and discuss science with many colleagues. With @cijilim.bsky.social @jenscs83.bsky.social, Stone, Nandakumar, Zappulla labs at banquet

I assume a revision of a review??

An intrinsically disordered region mediates RNA-binding selectivity and pro-oncogenic activities of LARP6 Intrinsically disordered regions (IDRs) are prevalent in RNA-binding proteins (RBPs), yet their roles in RNA interactions remain poorly defined. Here, we examined the structured and disordered RNA-bin...

🧵1/
🚨Check out our significantly updated preprint, now out on @biorxivpreprint.bsky.social. Together with Sasi Conte lab, we characterize a novel role for intrinsically disordered regions (IDRs) in promoting RNA-binding selectivity in RNA binding proteins (RBPs): www.biorxiv.org/content/10.1...

And on other news. My first contribution to science as a postdoc with @stirlingchurchman.bsky.social is out! We discuss the knowns and unknowns of post-transcriptional splicing. It was really fun to write with the great @karinechoquet

Screenshot reporting the Pubmed page of the review article published on Cold Spring Harbor Perspectives in Biology by our Lab.

Telomere Community 🧬 Can't wait for the new CSHL Monograph? Our review on unusual structure at Telomeric repeats is now live on Cold Spring Harbor Perspectives! Grab a few free PDFs here: cshperspectives.cshlp.org/cgi/reprint/... #CHSL #AcademicSky #Science @ezeta.bsky.social @cshlnews.bsky.social

I remember the announcer saying “What are they doing??? You can’t man Crab!!”.

Heavy deadlifts with bad form are a cure all for back issues 🤣🤣

Structural basis for DNA double-strand break sensing by human MRE11-RAD50-NBS1 and its TRF2 complex The MRE11-RAD50-NBS1 (MRN) complex is a central, multifunctional factor in the detection, signaling and nucleolytic processing of DNA double-strand breaks (DSBs). To clarify how human MRN binds generi...

Happy to share our latest pre-print!
We solved the cryoEM structures of human DNA repair factor MRN bound to DNA and to TRF2. Main work of Yilan, Filiz @filizkuybu.bsky.social and Hengjun. Great collab within the @sfb1361.bsky.social and @genecenter-lmu.bsky.social 🧬🔨
www.biorxiv.org/content/10.1...

The “Home alone” strategy :)!

Well done!

Plasmids for our destabilized MCP (dMCP) & PCP (dPCP) coat proteins are now on @addgene.bsky.social here: www.addgene.org/browse/article/28252367/

These proteins are degraded by cells except when bound to their RNA hairpin targets (MS2 & PP7): www.biorxiv.org/content/10.1101/2024.11.21.624393v1

100%. I used squarespace.

Derivatives 😁😁

Seems like this application should not have been discussed!

1.5 and 5 this should make for an interesting discussion. What was the final impact score???

Thursday is the second best day because you know Friday is next 😄

Cash lab is hiring a junior specialist/lab manager. Please feel free to reach out to me for details!
Link to apply here: recruit.ucdavis.edu/JPF07011
Lab website here: cash4rhogefs.faculty.ucdavis.edu

SPO11 dimers are sufficient to catalyse DNA double-strand breaks in vitro - Nature A biochemical system recapitulates the hallmarks of meiotic double-strand break formation, with mouse SPO11 catalysing break formation in the absence of any partners and remaining covalently atta...

🥳Just published @nature.com. Over 25 years after the discovery of SPO11 as the enzyme responsible for initiating meiotic recombination, we finally succeeded in reconstituting its DNA cleavage activity.🧵
www.nature.com/articles/s41...