chrysalis angel 🪻 🇵🇸

I know twitter is bad but if you're really good at it you can drive discourse to attack both transmisogynistic trans mascs and get far more trans mascs onto T by dispelling lies and myths about DIY transition at the same time. It's a beautiful thing to watch.

Governed

THIS IS HUGE! Researchers have just identified a powerful new antibiotic that is 100x STRONGER than existing drugs against deadly superbugs like MRSA and VRE. Hiding in a well-known soil bacterium, pre-methylenomycin C lactone KILLS drug-resistant bacteria WITHOUT triggering resistance.

Summary A team of scientists discovered a hidden antibiotic 100 times stronger than existing drugs against deadly superbugs like MRSA. The molecule had been overlooked for decades in a familiar bacterium. It shows no signs of resistance so far, offering hope in the fight against drug-resistant infections and paving the way for new approaches to antibiotic discovery.

Abstract The methylenomycins are highly functionalized cyclopentanone antibiotics produced by Streptomyces coelicolor A3(2). A biosynthetic pathway to the methylenomycins has been proposed based on sequence analysis of the proteins encoded by the methylenomycin biosynthetic gene cluster and the incorporation of labeled precursors. However, the roles played by putative biosynthetic enzymes remain experimentally uninvestigated. Here, the biosynthetic functions of enzymes encoded by mmyD, mmyO, mmyF, and mmyE were investigated by creating in-frame deletions in each gene and investigating the effect on methylenomycin production. No methylenomycin-related metabolites were produced by the mmyD mutant, consistent with the proposed role of MmyD in an early biosynthetic step. The production of methylenomycin A, but not methylenomycin C, was abolished in the mmyF and mmy mutants, consistent with the corresponding enzymes catalyzing the epoxidation of methylenomycin C, as previously proposed. Expression of mmyF and mmyo in a S. coelicolor M145 derivative engineered to express mmr, which confers methylenomycin resistance, enabled the resulting strain to convert methylenomycin C to methylenomycin A, confirming this hypothesis. A novel metabolite (premethylenomycin C), which readily cyclizes to form the corresponding butanolide (premethylenomycin C lactone), accumulated in the mmyE mutant, indicating the corresponding enzyme is involved in introducing the exomethylene group into methylenomycin C.

Remarkably, both premethylenomycin C and its lactone precursor were one to two orders of magnitude more active against various Gram-positive bacteria, including antibiotic-resistant Staphylococcus aureus and Enterococcus faecium isolates, than methylenomycins A and C, providing a promising starting point for the development of novel antibiotics to combat antimicrobial resistance.

Graphical Abstract

Chemists discovered a powerful hidden antibiotic that’s 100 times stronger than existing ones and effective against deadly superbugs.

The study has been published in the Journal of the American Chemical Society. YES, it is PEER-REVIEWED.
• pubs.acs.org/doi/10.1021/...

🧪🧵⬇️

I like the idea of them all being on different channels a mixer board

Help Jess recover from the devastating loss of her girlfriend, Willow. In the early hours of October 17th, Willow Totzke passed on. She leaves behind a devastated community, especially her longtime girlfriend, Jessica.

hey gang. if you could spare any cash or at least a repost this would mean a lot to me, and Willow's family/partner.
chuffed.org/project/1553...

Interesting coping method

Keep your goals high

The things we do for love

I have no idea what my job is. I spend a couple hours a day putting numbers into a database and then generating charts for people. And that's it. No idea what the numbers are or what the charts are for. The rest of the day I just wander around the building or scroll social media.

hey so me and the girls are gonna study your brain for science. We got lab coats and a clip board and everything

The effects of hormones and surgery change the recognized markers of sex

a guy holds out his phone and says “i love this app that lets you virtually pet a dog” another guy looks concerned and says: “don't they send 17 trillion dollars to israel every year”

Someone's screenshot of a two-post twitter thread, from user blue (@bluewmist): the fastest way to kill motivation is to make your identity depend on the outcome. it's called ego involvement. when failing becomes failing as a person, your brain starts avoiding the whole thing. not because you don't care, but because you care too much. you don't need lower standards. you need less self-worth tangled up in your goals. the work gets easier when it's not about proving who you are.

Found this on the AO3 subreddit, and I'm about to let it change my whole life (or at least work really hard to internalize it wrt my writing).

In a way it's depressing, everyone should get to live in a nice place but most this country is a Dollar General parking lot

yeah I love how conservative fantasies look like AI slop versions of Northampton, famously one of the gayest towns in America

Donate to Support Roxanne B.'s Journey to Stability, organized by Roxi B. Hi there. I'm Roxanne B. (the cat in the picture is Frank) I'm a disabled trans woma… Roxi B. needs your support for Support Roxanne B.'s Journey to Stability

Hey all- Roxanne here. Made a GoFundMe to try and build up some stability and pay off the outstanding debts that are causing a lot of my current issues. Link below. Any help is appreciated, as are reposts.

#mutualaid
#helpsky
#gofundme

gofund.me/05a32093a

Cats by Shou Xin

I like that the evil empire is crumbling but I don’t like that it’s where I live and that many large pieces of debris are going to land on me and the people I care about.

it makes sense that I'm nonbinary. my mom is a woman and my dad is a man. I got a bit of both . it's simply genetics

fox bone, glass beads, and spent shells necklace ❤️

just finished a necklace ❤️

some shit I've finished today ❤️

took some jewelry photos today ❤️

made a new set of earrings ❤️

some multimedia stuff I've been working on lately ❤️🧶

finishing this up 🧶❤️

more collage

mooooore