@attychang.bsky.social
PhD student in Grotjahn lab @Scripps Research #Teamtomo #Mitochondria
@attychang.bsky.social, @nanigrotjahn.bsky.social et al. show that cytoplasmic #ribosomes on #mitochondria alter the local membrane environment for protein import. rupress.org/jcb/article/...
๐ From The Year In Cell Biology: rupress.org/jcb/collecti...
#CellBio2025
Prohibitins arenโt hanging out just anywhere in the membrane โ they prefer the ๐โโ๏ธ๐โจVIP section๐
๐ชฉ๐ฅ
Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics.
s/o to #teamtomo #cryoET co-authors
@hamid13r.bsky.social
@attychang.bsky.social
@baradlab.com
Itโs been a big week for the Grotjahn Labโour very first graduate student, @mmedina300kv.bsky.social, successfully defended her PhD thesis! ๐ So proud of everything sheโs accomplished and grateful for the light sheโs brought to our lab from day one โ๏ธโก
10.05.2025 01:10 โ ๐ 27 ๐ 3 ๐ฌ 3 ๐ 1You want to start tomography? Solve structures inside cells? Reach Nyquist ๐ณ ? @phaips.vd.st and I have a website for you! tomoguide.github.io
You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software!
Hope it will be useful !
So excited for this work to be out!! Thanks to @attychang.bsky.social, @hamid13r.bsky.social, blue sky less Daniel Fuentes, @nanigrotjahn.bsky.social and @tomo.science!!!
01.05.2025 20:54 โ ๐ 13 ๐ 3 ๐ฌ 0 ๐ 0๐จNew preprint!๐จ #teamtomo
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ETโrevealing local variation across organelles.
Led by the labโs first grad student, @mmedina300kv.bsky.social (defending Monday! ๐พ) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
To all #ChlamyDataset enthusiasts, here is a script that lets you directly import the pre-processed results from EMPIAR-11830 into #RELION v5 for subtomogram averaging:
github.com/Chromatin-St...
#Chlamydomonas #TeamTomo #CryoET #OpenSoftwareAcceleratesScience
Figure 4. MemBrain v2 end-to-end workflow detects periodic phycobilisome organization. A: Raw tomogram slice of EMD-31244. B: Out-of-the-box MemBrain-seg segmentation (light blue). C: A single membrane instance can be visualized in Surforama and manually annotated with GT phycobilisome positions (magenta). D: MemBrain-pick localizes particles (trained with data from C) on all membranes in the tomogram. E: MemBrain-stats computes Ripleyโs O statistic using the positions from D with a bin size of 5nm. The distance between peaks (35 nm) was measured to estimate chain unit spacings.
We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples!
Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! ๐ง ๐งต
#CryoET #TeamTomo
www.biorxiv.org/content/10.1...
Thanks for your sharing!๐
23.04.2025 05:41 โ ๐ 1 ๐ 0 ๐ฌ 0 ๐ 0Feeling so honored and thrilled to see our work make it to the coverโwhat a milestone in my career! I canโt wait to receive the poster from JCB๐คฉ
10.04.2025 05:18 โ ๐ 15 ๐ 6 ๐ฌ 1 ๐ 0Happy to share the first paper in my PhD is published in JCB!
It's my honor to work with amazing teammates! I really enjoy exploring mitochondrial protein import mechanism through cryo-ET!
Cytoplasmic #ribosomes on #mitochondria alter the local membrane environment for protein import, say Ya-Ting Chang, Danielle Grotjahn (@nanigrotjahn.bsky.social) @scripps.edu and colleagues: rupress.org/jcb/article/...
#ProteinHomeostasis #Organelles #StructuralBiology #CryoET
