🚨Preprint alert🚨 Mapping of 3D collagen architecture with #tissueclearing, @mesospim.bsky.social #lightsheet #Microscopy, the Schmidt #multiimmersion microscope objective & #deeplearning in healthy tissue & wound healing by Wout Houbart & Thomas Naert & Co! www.biorxiv.org/content/10.6...

This amazing #microscopymonday capture of a young African turquoise killifish's brain blood vessels by the MDI Bio Lab's Haller lab was imaged using the MesoSPIM in the MDIBL Light Microscopy Facility.

#researchmatters #microscopy #fluorescentproteins #lightsheet 🧪 🐟🎸 🍎 👩‍🔬 🌎 🖥️ 🧬

A new preprint introduces aDISCO, a DISCO-based clearing approach that makes whole archival FFPE human tissues transparent and antibody-compatible, enabling true 3D light-sheet histology across brain & multiple organs at cellular resolution. Aguzzi & Helmchen teams.
www.biorxiv.org/content/10.1...

A high-resolution, easy-to-build light-sheet microscope for subcellular imaging An accessible light-sheet microscope delivers subcellular-resolution, multicolor volumetric, and live-cell imaging, lowering barriers to state-of-the-art performance.

In @elife.bsky.social: A high-resolution, easy-to-build light-sheet microscope for subcellular imaging doi.org/10.7554/eLif...

That's super cool, congrats! 🥳

(And that scan lens is essentially a really nice NA 0.125 microscope objective with 32 mm FOV and an accessible pupil location)

The sup material of that same paper includes their scan lens design - so it seems like they designed it as a combo.

The Handbook of optical systems, Vol 4: Survey of optical instruments, Ch 7 on Microscope Optics (p 646) gives a few more reasons (very good chapter btw).

The big exception are stereo-microscopes and macro-zoom systems: Building a zoom system that's fully telecentric across all magnifications is not that trivial, so usually, the way macro systems are designed is that they get more telecentric with higher NA (where the effects are more apparent)

Similarly, in a classical wide-field microscope, being non-telecentric would mean that objects get bigger or smaller as you focus up and down - so they get blurry and change size. That's quite annoying as well, especially for visual use

I'm quite sure that all of these are telecentric or as close as possible. If you have a correction collar and expect varying media, being non-telecentric would mean that you have medium-dependent magnification (because the chief ray get's refracted differently at the cover slip) - that's annoying

You can still design tube lens + objective as a matched pair in the latter case and it will make the whole thing a little easier to balance. As usual, it's a high dimensional design space and there's many solutions that can make biologists happy

A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging An optical microscopy approach with an ultra-large field of view but retained subcellular resolution allows simultaneous imaging of neural activity in widely dispersed brain regions.

This is what Dan Flickinger did for the system that became the Janelia/Thorlabs multiphoton mesoscope: elifesciences.org/articles/14472

In my view, the trade-off is not really worth it. My suggestion would be to not just design the objective, but a whole objective/tubelens/scan lens combo that works together - this way, you can balance aberrations (in particular chromatic etc.) between them.

Yes, you can force a different pupil location onto an objective - essentially that's what's happening in a misaligned laser scanning scope or an OPM with a wrongly matched O1/O2 combo. The trade-off is that you might see other artifacts - for example vignetting, higher aberrations...

The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue - Nature Methods The mesoSPIM is an open hardware axially scanned light-sheet microscope for the rapid imaging of large cleared samples with isotropic resolution.

This figure is from Sup Figure 1 of the 2019 @mesospim.bsky.social paper: www.nature.com/articles/s41...

Telecentricity will make integration into custom scopes much easier. Not being telecentric means that your magnification/FOV depends on the working distance: Now imagine building an OPM with it: If it's not telecentric, the lightsheet will change its lateral width along its propagation direction

Schematic on how to correct field curvature along light-sheet propagation axis.

We're still exploring how to improve LSFM performance with "big" detection air objectives, where field curvature often limits achievable FOV. Here, Steven introduces curvedASLM, a method to dynamically correct field curvature (along one axis) in axially scanned setups www.biorxiv.org/content/10.6...

The EMBO Practical Course "Hack your microscope" in Oeiras, PT, 20–25 Apr 2026 aims to innovate, democratize advanced #imaging technologies and shape the future of open-source #microscopy – Apply now!

Deadline: 12 Dec

https://meetings.embo.org/event/26-microscope
#EMBOmicroscope #EMBOevents 🧪

Supercool work! What's the difference between the lite version ( github.com/uhlmanngroup... ) and the original version ( github.com/uhlmanngroup... ) from a user perspective? Also, how complicated/painful would it be to extend this to 3D?

Say hi to Alain! Hope you all have a fun SfN!

As far as I understand, it's a complete redesign with the same specs and mechanical housing as the CRS8k to allow backward-compatibility.

The long wait is finally over: The @thorlabs.bsky.social 8 kHz resonant scanner is available 🔬! It would be lovely if there would be a 30 mm cube compatible & acoustically shielded mount as well, but I'm sure that's going to come eventually! #Microscopy www.thorlabs.com/newgrouppage...

Philipp Keller, Benquan Wang, and Dan Flickinger at Janelia have designed a mirror objective for expansion samples that has NA 1 over a 12 mm FOV - a superimpressive setup! The only thing that's out about it is a patent application: www.freepatentsonline.com/y2023/000398...

Precise, predictable genome integrations by deep-learning-assisted design of microhomology-based templates - Nature Biotechnology Genomic integration of DNA templates is made more precise through microhomology-focused design.

Thomas Naert recently published a paper on a toolbox for DNA editing with some Schmidt objective data: www.nature.com/articles/s41...

One of the people I'm indebted the most for creating stunningly beautiful #mesoSPIM samples was Martina Schaettin - who tragically passed away in March this year. To honor her memory, Elkhan Yusifov created an award - submit your best #microscopy images & videos! www.linkedin.com/company/mart...

Thanks to @nvladimus.bsky.social the @mesospim.bsky.social #lightsheet #Microscope community is now on forum.image.sc/tag/mesospim !

Definitely the most unexpected @mesospim.bsky.social #lightsheet #microscopy application of the past years: Hunting dark matter with a lightsheet microscope! #astronomy 🔭🔬https://arxiv.org/abs/2503.20732

#Deeplearning-based #CRISPR DNA integration and editing by Thomas Naert & Sören Lienkamp & Co with lots of @mesospim.bsky.social #lightsheet #Microscopy data of tadpoles (and some taken with the #Schmidt #multiimmersion objective prototype!) 🔬🐸 www.nature.com/articles/s41...

The @thorlabs.bsky.social LCPA2 lines are a little more straight, but a little wider as well...