Kurianlab

Dissecting the impact of transcription factor dose on cell reprogramming heterogeneity using scTF-seq - Nature Genetics This study introduces single-cell transcription factor (TF) sequencing, a single-cell barcoded and doxycycline-inducible TF overexpression approach that reveals dose-sensitive functional classes of TFs and cellular heterogeneity by mapping TF dose-dependent transcriptomic changes during the reprogramming of mouse embryonic multipotent stromal cells.

🧵1/ Excited to share our new paper introducing a new #singlecell assay: scTF-seq, a high-throughput single-cell approach to explore how transcription factor (TF) dose shapes cell identity and reprogramming outcomes. 🔗 www.nature.com/articles/s41... Big congrats to the entire team @EPFL & @SIAT_China

Towards a unified framework for the function of endoplasmic reticulum exit sites - Nature Reviews Molecular Cell Biology Endoplasmic reticulum exit sites (ERES) are specialized ER subdomains that regulate the export of secreted cargo. This Roadmap explores how ERES integrate biochemical and mechanical signals to coordinate trafficking and proposes a multidisciplinary strategy to investigate their function, including in disease.

ER exit sites are dynamic ER subdomains that integrate signals, reshape membranes and steer secretion. Twelve groups in the field came together to map their diversity, regulation, unanswered questions and future directions.
www.nature.com/articles/s41...

Lead by postdoc in the lab Ruiqi Ge and in collaboration with Bob Coffey’s lab, we are happy to share rPAL-seq for rapid and sensitive sequencing based profiling of glycoRNAs @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...

We are very pleased to announce our joint meeting with the German @gfeev.bsky.social and the Dutch Developmental Biology Societies, which will take place in Potsdam from the 10-13th of March 2026. Registration is now open! www.uni-potsdam.de/en/gfe-meeti...

We combine our yeast genetics experiments with the massive body of literature data to make a case for 40S scanning of 5’UTRs of by 1D diffusion (finalised version): m.rnajournal.cshlp.org/content/31/1...

Furloughed NIH worker: Left office with 'pit' in my stomach about future of our country On day three of the government shutdown, around 750,000 federal employees are furloughed as the White House threatens mass firings as early as today. Furloughed NIH Program Director Jenna Norton joins...

Shared how the greater shutdown of NIH science has been underway since January on @msnbc.com today.

We need Congress to stand firm to protect science, democracy and the American people.

www.msnbc.com/katy-tur/wat...

If you’re applying to your dream lab for an internship/PhD/postdoc, always send a second email 1-2 weeks after the first one if you don’t hear back.

I promise we will be grateful rather than annoyed. My email inbox is a disaster and I’m quite junior - and very few of us have secretarial support

1. I was so grateful to Alex Cooper for having Dr. Jane Goodall on Call Her Daddy earlier this year which brought Dr. Goodall’s life’s work and example to millions of young women who might have heard of her, or recognized her, but may not have known of the depth of her contribution.

🤯

land of the free, home of the brave......

Meiotic cohesin RAD21L shapes 3D genome structure and transcription in the male germline Deletion of the meiotic subunit RAD21L alters 3D chromatin structure and transcription in the mammalian male germ line.

🚨 New from our lab in #ScienceAdvances!

RAD21L cohesin acts as both a 3D genome architect and a transcriptional gatekeeper in the male germline. Its loss disrupts both structure and function—highlighting the tight link between genome folding and gene regulation

www.science.org/doi/10.1126/...

Maria Leptin re-appointed as ERC President! 🇪🇺

ERC Vice Presidents said:

This is excellent news, ensuring continuity at a critical moment. On behalf of the Scientific Council, we thank Maria for her leadership & strong commitment that has taken ERC to new heights.

👇
erc.europa.eu/news-events/...

A bittersweet day as another postdoc leaves to start an independent lab at St Jude’s Children Hospital.

Xiaoyu Chen led fearless work on developing a therapeutic approach for Timothy syndrome—now advancing toward clinical trial.

Primatologist Jane Goodall dies, aged 91 – DW – 10/01/2025 The Jane Goodall Institute said the renowned chimpanzee researcher died of natural causes at the age of 91.

A gem of a human left this world

www.dw.com/en/primatolo...

Congrats @sarawickstrom.bsky.social

Beautiful story

Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells - Nature Cell Biology McCreery, Stubb et al. show that mechano-osmotic changes in the nucleus induce general transcriptional repression and prime chromatin for cell fate transitions by relieving repression of specific differentiation genes.

How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇

Polysomes and mRNA control the biophysical properties of the eukaryotic cytoplasm. A recent study by the Weis lab shows that intracellular diffusion is enhanced when polysomes are disassembled or when mRNA levels are reduced in eukaryotic cells. More: biol.ethz.ch/en/news-and-...

Another fantastic story by Escobar lab, Institute for Genetics, Cologne

Fantastic paper from the Gehring lab, Institute of Genetics, Cologne

Mechanical forces across compartments coordinate cell shape and fate transitions to generate tissue architecture - Nature Cell Biology Villeneuve et al. report coordination of contractile forces during mammalian hair follicle development, with actomyosin contractility and mechanical forces from the epidermis and underlying tissue regulating placode invagination and Sox9 expression.

Third paper to highlight!
Here, Lisa Manning, @sarawickstrom.bsky.social, &c olleagues show contractility, pressure, signalling cooperating in vertebrate development, w/thorough combination of expertise in cell biology, signalling, & biophysics.

(Open Access) www.nature.com/articles/s41...

Top US researchers rush to relocate to Europe U.S.-based applicants to a prestigious EU research scheme have increased five-fold.

ERC funding for researchers wanting to move to Europe from the US has increased, now relocation grants available of up to €4.5 million over 5 years. Applications up 5x.
www.politico.eu/article/euro...

Rookie scientists make research teams more innovative Papers from research teams with a substantial number of beginners are highly disruptive and innovative, study shows.

And diverse teams... www.nature.com/articles/d41... #disruption

Heat shock induces silent ribosomes and reorganizes mRNA turnover https://www.biorxiv.org/content/10.1101/2025.09.26.678833v1

Our annual event at Norwich RNA Club: AI in RNA Biology. Great speakers! Please join us this Thursday at 2pm @norwichrnaclub.bsky.social @johninnescentre.bsky.social @earlhaminst.bsky.social @uniofeastanglia.bsky.social @alperakay.bsky.social

Germany's Helsing unveils 'Europa' combat drone German defence startup Helsing unveiled a new autonomous combat drone, joining a race to build remote-piloted systems that can swarm into battle with fellow robots or team up with crewed fighters to help reshape the future of air warfare.

...we fund more to kill than to live

When a society pours more time and money into more creative ways to kill fellow humans than into better lives or a livable planet, we’re off course.

www.reuters.com/business/aer...

Dear @dfg.de & @erc.europa.eu Why don't you do the same? What's stopping us from adopting this model to all tax-payer funded research in Europe? journals.plos.org/plosbiology/...

Congrats buddy!!

Wow!

🚀 Our new paper is out @natmethods.nature.com!

Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.

🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/

The eukaryotic replisome intrinsically generates asymmetric daughter chromatin fibers DNA replication is molecularly asymmetric, due to distinct mechanisms for lagging and leading strand DNA synthesis. Whether chromatin assembly on newly replicated strands is also asymmetric remains un...

Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)