Pedro Escoll

Backtracking metabolic dynamics in single cells predicts bacterial replication in human macrophages
@natcomms.nature.com
www.nature.com/articles/s41...

Great work! HCS❤️AI

Thus, single-cell metabolic dynamics can forecast infection outcomes. In the spirit of @stephenwolfram.bsky.social's ideas, bacterial infection exhibits pockets of computational reducibility at the single-cell scale. Might this point to new ways to predict infection progression?

So we trained an explainable Machine Learning model using Δψm + mROS dynamics, and the AI model predicted which infected cell will support replication with 83% accuracy before bacterial replication begins

What make these permissive macrophages different? Before bacteria replicate, macrophages that will permit bacterial growth already show sustained mitochondrial membrane potential (Δψm) and higher mitochondrial ROS (mROS). These are early mitochondrial signals of permissive cells!

Backtracking metabolic dynamics in single cells predicts bacterial replication in human macrophages - Nature Communications Computational models can help to explain the dynamics of cellular infection with pathogens. Here the authors use computational models to assess the single cell infection parameters of human macrophage...

We tracked thousands of living primary human macrophages during infection with Legionella pneumophila, using high-throughput live imaging + our BATLI backtracking software. Legionella only replicates in ~20% infected primary macrophages!
www.nature.com/articles/s41...

New paper finally out on @natcomms.nature.com! Led by first author Maria Dramé with the collaboration of Francisco Garcia, @cbuch.bsky.social and @jytinevez.bsky.social at @pasteur.fr. Early mitochondrial signals in single human macrophages predict later Legionella replication. Link + details👇🧵

Extracellular ATP is an environmental cue in bacteria Extracellular ATP acts as a signal regulating physiology and immunity in eukaryotes and is elevated during intestinal infection or damage. Tronnet et al. show that extracellular ATP reprograms the tra...

Extracellular ATP is an environmental cue in bacteria

@cp-cellreports.bsky.social

www.cell.com/cell-reports...

Research engineer in Bioimage Analysis for the researchers of the inIdEx FORMULA Hi all We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks? The position is in the Instit...

Job alert!

We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks?

See also on the forum:
forum.image.sc/t/research-e...

New preprint in the lab! How the mitochondrial ETC works during infection of human macrophages with #Legionella or #Salmonella? Spoiler: there are some similarities and also pathogen-specific differences. Work led by Francisco Garcia and Paula Martínez, with @cbuch.bsky.social at @pasteur.fr

🦠 Mycobacteria aren't just tough, they're interior designers! 🦠 Check out our latest #preprint 🚨, led by our PhD student @camimille.bsky.social , where we show that #Mycobacterium tuberculosis builds secret cytoplasmic membranes to adapt its metabolism and sneak past immunity 🤫

Thread coming soon!

© Institut Pasteur

🟢 The Institut Pasteur goes green for Mitochondrial Disease Awareness Week! 🟢
These rare diseases affect 1 in 5,000 people. Mitochondria, the “powerhouses” of our cells, are also thought to play a role in Alzheimer's disease.
Research today, cure tomorrow. 🔬 #WorldMitoWeek2025

Hydrogen sulfide production distinguishes Salmonella from close relatives, but its biological significance remains obscure. This study uncovers the secret: Salmonella uses hydrogen sulfide production as a weapon to outcompete E. coli and gain a foothold in the gut.
www.pnas.org/doi/10.1073/...

Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters Uridine boosts aminoglycoside treatment efficiency against antibiotic-susceptible as well as antibiotic-resistant E. coli strains.

So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!

New preprint in the lab! How the mitochondrial ETC works during infection of human macrophages with #Legionella or #Salmonella? Spoiler: there are some similarities and also pathogen-specific differences. Work led by Francisco Garcia and Paula Martínez, with @cbuch.bsky.social at @pasteur.fr

Complex-I Preserves Mitochondrial Polarization during Infection of Human Macrophages by Secretion-competent Bacteria https://www.biorxiv.org/content/10.1101/2025.09.04.674018v1

That concludes GRC Salmonella 2025. What a fantastic meeting it was - topnotch science, great discussions and a wonderful spirit. I am proud to be part of this amazing scientific community.

A Salmonella T3SS-2 mutant grows fine in spleen macrophages, contradicting tissue culture dogma (PMID: 23236281). This observation was largely ignored, but adding certain carbon sources rescues growth in cultured macrophages, hinting that T3SS-2 may be doing something entirely different in vivo.

Shigella-trained pro-inflammatory macrophages protect zebrafish from secondary infection
@cgmargarida.bsky.social

New pre-print in the lab! Beautiful work by Francisco Garcia-Rodriguez in collab with @kamovalenz.bsky.social
and @joaquinbernal.bsky.social : when macrophages are provided with specific nutrients, Salmonella can bypass its requirement of the T3SS to replicate, a process that occurs within the SCV

New study reveals that Salmonella can replicate inside macrophages even without its T3SSs, if the nutrient conditions are right! Metabolic heterogeneity of host cells is a game-changer in bacterial replication dynamics. #MicroSky

✍️ @pescoll.bsky.social and coll. @pasteur.fr

📖 shorturl.at/a9a06

Congrats Lea Jost and team!! Really beautiful paper!!

Our latest paper is out! Congratulations to @leaswistak.bsky.social et al for this amazing #cryoET work that elucidates the initial stages of #T3SS dependent vacuolar damage during #Shigella infection. We will do a deep-dive later! journals.plos.org/plosbiology/...

Provision of Preferred Nutrients to Macrophages Enables Salmonella to Replicate Intracellularly Without Relying on Type III Secretion Systems https://www.biorxiv.org/content/10.1101/2025.05.15.653970v1

New pre-print in the lab! Beautiful work by Francisco Garcia-Rodriguez in collab with @kamovalenz.bsky.social
and @joaquinbernal.bsky.social : when macrophages are provided with specific nutrients, Salmonella can bypass its requirement of the T3SS to replicate, a process that occurs within the SCV

Another very cool story I was lucky enough to be involved in! Congrats @pescoll.bsky.social and Francisco! Now let’s hope for a smooth submission!

Congrats Jose and team!!!!!

Mobile integrons encode phage defense systems Integrons are bacterial genetic elements that capture, stockpile, and modulate the expression of genes encoded in integron cassettes. Mobile integrons (MIs) are borne on plasmids, acting as a vehicle ...

🧪
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉

Short 🧵
www.science.org/doi/10.1126/...

Hello world !