Kristina Micheva

From @bdpedigo.bsky.social: a super efficient, highly reliable, and very generalizable method for mesh structure classification, applied here to spine detection across basically every synapse onto a cell in the MICRoNS dataset.

The GeneTex GTX13970 works well for me, on glutaraldehyde fixed tissue as well. For TdTomato: Origene AB8181. Both used postembedding, Lowicryl HM20

Portrait of Dan V. Madison at an electrophysiology rig, overlayed on pairs of pyramidal neurons filled and reconstructed.

It is with a heavy heart that I share the unexpected news that our colleague, friend, and mentor Daniel Madison passed away on Thursday, October 9.

E11 Bio is excited to unveil PRISM technology for mapping brain wiring with simple light microscopes. Today, brain mapping in humans and other mammals is bottlenecked by accurate neuron tracing. PRISM uses molecular ID codes and AI to help neurons trace themselves.

Read more: e11.bio/blog/prism

A sprouted sweet potato puts a gorgeous display of delicate pinkish-purpelish stems and leaves

Back from a trip and I found this unintentional present I left myself

We should definitely think and talk more about this! Ignoring these blind spots can be damaging to both our science and our morality. Some of us try to appease our conscience by sparing other animal lives - gently taking spiders outside, becoming vegetarian, but that does not resolve the conflict

It was a good experience to step back and briefly take stock of the amazing progress in connectomics since I started working on this stuff (20 years ago!)

thanks as well to @natrevneuro.nature.com for the constructive editorial interactions.

Ultramicrotomy escapism: trimming blocks and enjoying the shapes and colors 💕

🤣

Workshop 2025 About the workshop This workshop will cover array tomography (AT) technology with a focus on “simple and affordable” workflows based on serial ultramicrotomy and imaging. The practicals include...

Our 2nd Array Tomography Workshop will be in November in Buenos Aires and there is still time to apply! Five days of hands-on learning, discussions and talks + gorgeous electron microscopy and immunofluorescence. Focus on simple and affordable workflows.
www.arraytomography.org/workshop-202...

MBL Announces Largest Ever Gift for Its Renowned Biological Education and Research Programs | Marine Biological Laboratory Cell Biology Researcher Mark Terasaki’s $25 Million Unrestricted Gift Will Support Operations and Infrastructure Contact: Samantha Cummis scummis@mbl.edu; 973.800.4119

We're thrilled to announce the largest private gift in our 137-year history: a $25 million unrestricted donation from MBL Whitman Scientist Mark Terasaki.

His extraordinary generosity will bolster our renowned research and education programs. 🔬

Learn more: bit.ly/452WL1m

Congratulations!

Excited to announce registration to the next Array Tomography workshop in Buenos Aires is now open!

#volumeEM

See our AT website page for more information ⬇
www.arraytomography.org/workshop-202...

Distinct domanial and lamellar distribution of clustered lipofuscin granules in microglia in the main olfactory bulb of young mice - PubMed Lipofuscin granules are generally considered as age-pigment. However, we encountered numerous large irregular clusters of lipofuscin granules in the olfactory nerve layer and glomerular layer of the m...

Looks like autofluorescence to me (not an olfactory expert). Could it be lipofuscin, I see that a lot in cortex where I mostly look? A quick search of lipofuscin and olfactory bulb brought this paper and the images look pretty similar to yours: pubmed.ncbi.nlm.nih.gov/19666062/

Congratulations! You are amazing!

Congratulations!

Activity-driven myelin sheath growth is mediated by mGluR5 - Nature Neuroscience Employing pharmacology, genetics and all-optical approaches in zebrafish, Braaker et al. find that neuronal activity influences the growth of myelin sheaths along axons by signaling through metabotrop...

For those who want a distraction and/ or are interested, here's the latest from the group, led by Phil Braaker

www.nature.com/articles/s41...

I'm so sorry, there's no words

Congratulations! Looking forward to all the exciting work to come from this!

+1

Reminder that @europepmc.org is a mirror of Pubmed with better search and that it indexes *all* bioRxiv preprints (as well as ResearchSquare), not just the ones funded by NIH like chauvinist Pubmed

Congratulations! Can't wait to read it!

What happened to the pursuit of truth? rupress.org/jgp/article/... - Eve Marder, on point...

Delighted!!! Thank you!

Looks like a perfect winter treat, but not naming it to keep the suspense going 😂

And welcome to @owen-lab.bsky.social who is new here!

Happy to share a new biorxiv manuscript with @owen-lab.bsky.social where we “slice and dice” synapses with array tomography and find subclasses with different glutamate receptor composition 🧪

Gif showing a spine containing spine apparatus, reconstructed from FIB-SEM images. ER: red, synapse: yellow, plasma membrane: blue

Our paper is out just in time for #EM_Monday! It is about a specialized neuron-specific ER, called the spine apparatus, which is found close to synapses and has a very peculiar shape. If you think neurons are special, the spine apparatus is their🦄 horn! But how does it form?!
doi.org/10.1016/j.cu...

A section of retina through the optic fiber layer, ganglion cell layer, inner plexiform layer, inner nuclear layer with amacrine and bipolar cells, the outer plexiform layer and the outer nuclear layers of retina. This has been labeled with antibodies against small molecules to visualize the metabolism using Computational Molecular Phenotyping (CMP) strategies.

Layers Upon Layers

bryanwjones.com/2020/06/laye...

#SciArt #retina

Get a free NIH BRAIN Initiative art calendar to prep for 2025: catalog.ninds.nih.gov/publications... .

I'm proud to say that the cover is a piece we created from the DTI imaging of a subject in the BRAIN funded depression DBS study we reported last year: go.nature.com/48lmlzC

🧪🧠