Dimitris Kolokouris

More exciting Cationic Amino acid Transporters: The more promiscuous and pH-regulated SLC7A4.

Beautiful work from our friends in the Newstead and @philbiggin.bsky.social labs down the hill of @oxfordbiochemistry.bsky.social

www.researchsquare.com/article/rs-7...

Thanks for sharing our work David!

Upper - cryo-EM reconstruction of stomatin AQP1 complex. Lower - reconstruction of stomatin-UT-B complex.

Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️

Covalently constrained ‘Di-Gembodies’ enable parallel structure solutions by cryo-EM - Nature Chemical Biology Disulfide-based dimerization of modified identical and heterologous nanobody scaffolds enables higher-order assembly for high-resolution cryo-electron microscopy structure determination that is widely...

Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets!

A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social

Out now in Nature Chemical Biology @natchembio.nature.com

www.nature.com/articles/s41...

Cryo-EM structures of a pentameric ligand-gated ion channel in liposomes pubmed.ncbi.nlm.nih.gov/40502198/ #cryoEM

Membrane protein shown with part of micelle density removed to reveal transmembrane protein regions.

LocScale-SURFER is a ChimeraX extension that shows cryoEM density maps after removing detergent micelles based on output from command-line program Locscale-2.0. Install it using ChimeraX menu Tools / More Tools.... More details at cxtoolshed.rbvi.ucsf.edu/apps/chimera...

Single-particle cryogenic electron microscopy structure determination for membrane proteins pubmed.ncbi.nlm.nih.gov/40228430/ #cryoEM

Structural basis for cholesterol sensing of LYCHOS and its interaction with indoxyl sulfate. @NatureComms Check the #cryoEM #structure of this #membrane #protein in the UniTmp database: https://pdbtm.unitmp.org/entry/9j3x

Is the difference between AlphaFold and physical/bench structure determination methods the same as the difference between sex on the screen and sex with a real person?
#AI #MolecularBiology

If you do biochemical simulations/comp. biophysics, this MD starter pack has all the right people 🚀

Hi I do membrane protein lipid interactions. Can you add me here?

Hi I’d like to be part of the community. I do cryoEM of membrane proteins and single molecule fluorescence!

It's nice to have this back here !

Bluesky bot that surveys your literature research subfield 👍 #CryoEM

@cryoempapers.bsky.social

Congrats on the beautiful work

Hi can you add me and @iriskalen.bsky.social ?

Hi please add me along with:
@iriskalen.bsky.social

Hi I’d like to be added!

Super cool work!

Proud of new work tinyurl.com/yhz4c6ry from the lab by Zhicheng (Chen) Cui using single-particle cryo-EM on liposomes to show how mTORC1 integrates nutrient and growth factor signaling at lysosomes, collab. w/
G. Napolitano,A. Esposito, and A. Ballabio.

Thanks for catching this Robin, very relevant!

Hi I’d love to join! 🧬🎉

I kind of forgot how to announce Feeds, but here goes

For an all-in-one CryoEM Feed:

#CryoEM, #CryoET, #TeamTomo all-in-one feed that curates posts from the last 7 days containing the various cryoEM and cryoET related hashtags and keywords.

bsky.app/profile/did:...

It was very cool work!