Claire Koenig

YouTube video by European Partnership for Personalised Medicine OVA-PDM - Personalising clinical decisions in ovarian cancer through patient-derived in vitro models

We made a short video for the ERA PerMed video competition 🎬
We present our collaborative project on “personalising clinical decisions in ovarian cancer through patient-derived in vitro models”.
Have a look at it 👇🏼 and don’t forget to suport us by putting like on Youtube 👍🏼

youtu.be/J4bc1MPqIqE?...

Enhancing Tandem MS Sensitivity and Peptide Identification via Ion Preaccumulation in an Orbitrap Mass Spectrometer High-throughput mass spectrometry-based proteomics has gained increasing interest for both academic and industrial applications. As implementation of faster gradients has facilitated higher sample thr...

Boosting Orbitrap MS/MS speed to ∼70 Hz? 🚀

Read how we did it in our latest paper 👇

pubs.acs.org/doi/10.1021/...

Thanks a lot! I love working with you and calling you my friend 🥰

Today at CNIC we had the pleasure to host @clairekoenig.bsky.social for her talk about "Proteins for the past". It sparked a lot of interest!

The paper is actually intended to be open access and we are working on it 🔓

🚨 Very exited to see our project published in @science.org
We used proteomics on Paranthropus robustus fossils (~2 million years old) to determine the sex of individuals and detect genetic variability, offering new insight into potential subgroups within this ancient hominin 🦷

Assessing the potential of ancient protein sequences in the study of hominid evolution Palaeoproteomic data can provide invaluable insights into hominid evolution over long timescales. Yet, the potential and limitations of ancient protein sequences to resolve evolutionary relations betw...

We’ve released a preprint on #palaeoproteomics and #phylogenetics !

Over the years, I’ve gotten multiple questions on the usefulness of ancient proteins in resolving phylogenetic questions. This is our attempt to provide some answers:

www.biorxiv.org/content/10.1...

Global analysis of protein turnover dynamics in single cells The SC-pSILAC method enables single-cell measurement of both protein abundance and turnover, providing notable advances in the depth and versatility of proteomic technologies.

🚀 Excited to share our latest article in #singlecell proteomics published in Cell!
We've developed SC-pSILAC to simultaneously measure protein turnover and abundance in single cells, unlocking the first large-scale, 2D proteomic insights at single-cell resolution!

www.cell.com/cell/fulltex...

Hi

We contributed towards the South African Journal of Science Taung Centennial!

some links;

the special issue: issuu.com/sajs/docs/so...

our paper: sajs.co.za/article/view...

and I got featured on where I work from Nature Africa if you are interested in that: www.nature.com/articles/d44...

Formaldehyde Fixation Helps Preserve the Proteome State during Single-Cell Proteomics Sample Processing and Analysis Mass spectrometry-based single-cell proteomics (SCP) is gaining momentum but remains limited to a few laboratories due to the high costs and specialized expertise required. The ability to send samples to specialized core facilities would benefit nonspecialist laboratories and popularize SCP for biological applications. However, no methods have been tested in SCP to “freeze” the proteome state while maintaining cell integrity for transfer between laboratories or prolonged sorting using fluorescence-activated cell sorting (FACS). This study evaluates whether short-term formaldehyde (FA) fixation can maintain the cell states. We demonstrate that short-term FA fixation does not substantially affect protein recovery, even without heating and strong detergents, and maintains analytical depth compared with classical workflows. Fixation also preserves drug-induced specific perturbations of the protein abundance during cell sorting and sample preparation for SCP analysis. Our findings suggest that FA fixation can facilitate SCP by enabling sample shipping and prolonged sorting, potentially democratizing access to SCP technology and expanding its application in biological research, thereby accelerating discoveries in cell biology and personalized medicine.

🚨 More research on single-cell proteomics!
We evaluated how formaldehyde-based fixation preserve proteome state, drug response and cell integrity. Ultimately, cell fixation can facilitate access to #singlecell by enabeling sample shiping and prolonged sorting 📦

pubs.acs.org/doi/10.1021/...

Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics - Nature Methods Chip-Tip is a label-free quantification-based single-cell proteomics workflow for deep single-cell proteomics, which identifies over 5,000 proteins and 40,000 peptides in single HeLa cells.

🚨 Our #singlecell article is out! We describe the Chip-Tip label-free SCP workflow quantifying >5000 proteins in single HeLa cells. With high scalability, increased depth and throughput we can now envision large-scale LFQ-SCP biomedical studies!
www.nature.com/articles/s41...

Well, let’s see in the next few days :)