Osvaldo Contreras osvaldoics

Congratulations Dr Osvaldo Contreras

Congratulations @osvaldoics.bsky.social on being awarded a MAWA/RFF Research Grant - supporting his project Animal Free Human Stem Cell Models to Investigate Congenital Heart Defects. Grateful to the MAWA Trust and the Rozzoli Family Fund for supporting this work.

27.02.2026 00:10 — 👍 6 🔁 2 💬 0 📌 0

Thanks for sharing! 🙏🏽😃

21.01.2026 10:30 — 👍 1 🔁 0 💬 0 📌 0

ICYMI
🔗: www.biorxiv.org/content/10.6...

21.01.2026 10:08 — 👍 2 🔁 1 💬 1 📌 0

Proteo-transcriptomics and morphometrics of teleost cardiac cells define regulatory networks and exercise-induced cardiomyocyte hypertrophy and hyperplasia Zebrafish and medaka are powerful cardiovascular models, yet cellular and molecular investigations of adult heart cells have been constrained by suboptimal dissociation and characterization methods. To overcome these barriers, we developed a physiological-temperature workflow that generates high-yield, viable single-cell suspensions for FACS, imaging, and low-input molecular profiling. Using transgenic fluorescent reporters, we consistently isolate ∼6,000 cardiomyocytes per adult zebrafish ventricle and ∼12,000 from medaka, preserving cellular, structural, and molecular integrity. Single-cell morphometrics revealed cardiomyocyte heterogeneity and demonstrated that swimming exercise induces both hypertrophy and hyperplasia, while ventricular injury triggers expansion of regenerative gata4 ⁺ cardiomyocytes. We integrated proteomics and RNA-seq from FACS-purified cells to construct cell-type-specific proteo-transcriptomic atlases. Functional enrichment, transcription factor, and network analyses identified protein hubs and regulatory circuits defining cardiomyocyte and endothelial cell identity. Our platform delivers cell-type-resolved molecular datasets of adult teleost cardiac cells, establishing a systems-level resource for heart regeneration research, cardiovascular disease modeling, and drug discovery. Teaser A robust workflow for isolation, FACS, imaging, and multi-omics profiling of cardiomyocytes and cardiac endothelial cells in fish. HIGHLIGHTS ### Competing Interest Statement The authors have declared no competing interest.

www.biorxiv.org/content/10.6...

18.01.2026 22:44 — 👍 1 🔁 1 💬 0 📌 0

I’m back here to share our new pre-print! 🔬 🧬 😃

Proteo-transcriptomics and morphometrics of teleost cardiac cells define regulatory networks and exercise-induced cardiomyocyte hypertrophy and hyperplasia

Flow cytometry+Imaging+RNA-seq+Proteomics

Preprint available on bioRxiv [link in comments]

18.01.2026 22:44 — 👍 15 🔁 5 💬 1 📌 1

Thanks for the highlight Prof Deepak!

15.12.2025 05:32 — 👍 1 🔁 0 💬 0 📌 0

Fantastic insights on how replication stress drives cell differentiation by Osvaldo Contreras at the joint meeting of the #InSDB and International Society of Differentiation #ISD joint meeting at #IISER_BERHAMPUR
@osvaldoics.bsky.social
#INSDBISD2025

15.12.2025 05:29 — 👍 2 🔁 1 💬 1 📌 0

Congrats!

10.12.2025 23:14 — 👍 0 🔁 0 💬 0 📌 0

Star Scientist - Dr Osvaldo Contreras The Institute's Dr Osvaldo Contreras's work could one day help children born with heart defects and adults facing heart failure live longer, healthier…

Star #Scientist @osvaldoics.bsky.socia is uncovering how hearts grow - and what goes wrong when they don’t. He's mapping the molecular ‘blueprint’ of heart cells to uncover the mechanisms that could one day help damaged hearts repair themselves:

10.12.2025 04:10 — 👍 3 🔁 1 💬 0 📌 0

Thank you for the highlight @ishr-aus.bsky.social 🙏🏼❤️

03.12.2025 22:47 — 👍 3 🔁 2 💬 0 📌 0

Cannot agree more

03.12.2025 22:30 — 👍 0 🔁 0 💬 0 📌 0

Nuclear actin and DNA replication stress regulate telomere maintenance by telomerase - Nature Communications Telomerase recruitment to telomeres is a tightly regulated process which is stimulated by replication stress. Here, the authors identify that nuclear filamentous actin is important for interaction bet...

New @natcomms.nature.com paper today from Tracy Bryan's lab @cmri.bsky.social: "Nuclear actin and DNA replication stress regulate telomere maintenance by telomerase".

Happy our lab could play a small part.

Congrats Tracy, Ash Harman, Noa Lamm and the whole team.

www.nature.com/articles/s41...

03.12.2025 00:19 — 👍 22 🔁 13 💬 2 📌 0

💡Dr Osvaldo Contreras’s funds will allow for open access publication of “OpenEMMU: A versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics” in iScience.

03.12.2025 22:26 — 👍 4 🔁 1 💬 1 📌 0

We are all mosaics: vast genetic diversity found between cells in a single person Technical advances allow researchers to trace the genetic changes that occur over time.

“There were some cells in there that were very messed up”

In a technological tour-de-force, researchers have sequenced the whole genomes of more than 100 individual cells from one 74-year-old man
go.nature.com/44yj4MN

26.11.2025 09:28 — 👍 73 🔁 20 💬 3 📌 2

Cool work!

19.11.2025 23:28 — 👍 0 🔁 0 💬 0 📌 0

In vivo differentiation of embryonic cells devoid of key reprogramming factors Youlten, Miao et al. use zebrafish transplantation to understand the fate of cells lacking pluripotency factors Nanog, Pou5f3, and Sox19b (NPS) in developmental reprogramming and cell identity establishment. They found that NPS are required for proper cell identity for most, but not all, lineages, likely through nuclear and cytoplasmic reprogramming.

In vivo differentiation of embryonic cells devoid of key reprogramming factors

12.11.2025 20:50 — 👍 5 🔁 3 💬 0 📌 1

Catch up on Development presents… webinar on development across scales - the Node Our October webinar featured two early-career researchers working on development across scales. Here, we share the talks from Osvaldo Contreras (Victor

thenode.biologists.com/catch-up-on-...

A webinar of our work is now available on YouTube 🔭

14.11.2025 12:02 — 👍 1 🔁 0 💬 0 📌 0

Biological Differentiation Across the Scales • November 14, 2025, at 12:00 p.m. Central • Register Now

One week left to register! Join the Editors-in-Chief of Differentiation on November 14 at 12:00 p.m. Central for a virtual seminar with Pavani P. Perera on the enteric nervous system, evolution, and cave fish. Register for free: https://bit.ly/4oaU71P
Topic: https://bit.ly/478UZhu

07.11.2025 19:49 — 👍 4 🔁 3 💬 0 📌 2

Stem Cell & Developmental Biology Early Career Symposium September 23-25, 2026 Seattle, Washington, USA Planning Committee: Alessandro Bertero, Ken Cho, Gideon Dunster, Whitney Edwards, Evan Graham, Valentina Greco, Leigh Harris, Serge Parent, Helen Willsey Allen Institute logo; International Society for Stem Cell Research logo; Society for Developmental Biology logo

Save the date! @isscr.org, @alleninstitute.org & @socdevbio.bsky.social are collaborating to present a 3-day scientific symposium led by early-career scientists. The Stem Cell & Developmental Biology Early Career Symposium will be held September 23-25, 2026 in Seattle, WA. Learn more: bit.ly/4p98dkv

13.11.2025 17:20 — 👍 24 🔁 18 💬 0 📌 2

Catch up on Development presents… webinar on development across scales - the Node Our October webinar featured two early-career researchers working on development across scales. Here, we share the talks from Osvaldo Contreras (Victor

Catch up on the recordings from our October #DevPres webinar on development across scales. View the presentations from @osvaldoics.bsky.social and @yinanwan.bsky.social.

thenode.biologists.com/catch-up-on-...

12.11.2025 10:13 — 👍 4 🔁 1 💬 0 📌 1

Transitions is the newest in our series of stem cell conversations. Launched by Ritika Saxena, Jarmon Lees and @osvaldoics.bsky.social we explore project and career transitions. All the parts needed for successful science that don't make it to the bench. For Aussie scientists, but all welcome.

19.10.2025 22:11 — 👍 2 🔁 1 💬 0 📌 0

We are celebrating 5 years of the Australia stem cell conversation! Hosted by @unimelb.bsky.social and @asscr.bsky.social the stem cell conversation is a weekly 30min virtual seminar series organised by EMCR for EMCR.
Join the first 'transitions' episode Leadership through Service: 11am AEST Wed.

19.10.2025 22:05 — 👍 6 🔁 2 💬 1 📌 1

I’m very excited to be invited to present at “Development Presents…”

Thanks @amjeve.bsky.social

30.09.2025 11:36 — 👍 6 🔁 2 💬 0 📌 0

Remarkable conference organized by @asbmbaus.bsky.social up in Brisbane at the University of Queensland.

I was invited to present my latest work as a Keynote Speaker for the Multi-Sonic’s session, and so many thanks for selecting me!

Truly an honour!

30.09.2025 01:13 — 👍 5 🔁 1 💬 0 📌 0

SAVE THE DATE! Join us for the 2025 @anzscdb.bsky.social
NSW Branch Cell & Developmental Biology Symposium at the Garvan Institute @garvaninstitute.bsky.social on Wed 5 Nov 2025. Keynote speakers include Dougall Norris, @katequinlan.bsky.social, Silvia Velasco and @ryanlister.bsky.social 🔬🧠🧫

19.09.2025 07:29 — 👍 8 🔁 2 💬 1 📌 1

Honored to present at @ramacgenomics.bsky.social @unsw.edu.au next Tuesday.😃

05.09.2025 03:55 — 👍 3 🔁 2 💬 0 📌 0

Don't miss @osvaldoics.bsky.social in the flesh next Tuesday in Sydney

05.09.2025 01:00 — 👍 1 🔁 1 💬 0 📌 0

Thank you Françoise!☺️

03.09.2025 10:06 — 👍 1 🔁 0 💬 0 📌 0

We're proud to share Dr Osvaldo Contreras has been awarded a Scale Biosciences Core Lab Grant, from @garvaninstitute.bsky.social Genomics Platform, Scale Biosciences, and Integrated Sciences to further his research into the origins of congenital heart disease that affects one in 100 babies.

01.09.2025 00:49 — 👍 7 🔁 1 💬 0 📌 0

OpenEMMU: A versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics Biochemistry; Cell biology; Developmental biology; Computational bioinformatics

Thrilled to share our new paper: "OpenEMMU: A versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics"!

We've developed a new resource that make this crucial research more accessible, accurate, powerful and low-cost.

www.cell.com/iscience/ful...

02.09.2025 21:44 — 👍 9 🔁 5 💬 2 📌 1

Posts by Osvaldo Contreras (@osvaldoics.bsky.social)