Oxford Optronix

In Vitro Dissolved Oxygen Monitoring in Hypoxia Chambers Explore how an in vitro system that can be utilized within hypoxic chambers ensures precise dissolved oxygen control in cell cultures and media. Hypoxia chambers are used worldwide by research investi...

Stop guessing pericellular oxygen and just measure it.

Guide to dissolved O2 in hypoxia chambers: think in mmHg, verify in media pO2, track stability with #OxyLite and #HypoxyLab.

Read here: www.oxford-optronix.com/resources/in...

#pO2 #Hypoxia #ResearchTools

What if oxygen is the switch for FLASH at the molecular scale?
At 5% O₂, UHDR yields fewer DNA strand breaks than LDR & at ~21% O₂ that advantage vanishes. #HypoxyLab set ~40 mmHg O₂ and #OxyLite confirmed O₂ and sealing to keep conditions true.

Read here👉 arxiv.org/pdf/2510.15478

Regional venous-to-arterial carbon dioxide pressure and content differences during endotoxemic shock: influence of hydrogen ion accumulation vs. Haldane effect - Intensive Care Medicine Experimental Background The relationship between carbon dioxide pressures (PCO2) and contents (CCO2) is linked to the Haldane effect. Nevertheless, under shock conditions, hydrogen ion accumulation might strongly ...

What if ΔPCO₂ in shock is more about acidosis than Haldane effect?
New study shows hydrogen ions dominate the CO₂ gap while flow recovers. #OxyFlo measured jejunal microvascular flow to link perfusion with CO₂ dynamics.
#Shock #CriticalCare #CO2 #LDF #Physiology

link.springer.com/article/10.1...

Rapidly dissolving polymeric microbubbles promote supersaturation and in-situ formation of acoustic nanobubbles capable of dynamic pressure sensing Developing noninvasive methods for monitoring intravascular and intracardiac pressures is crucial for advancing the diagnosis and management of cardio…

Novel paper by a @harvard.edu team used nanobubbles to convert pressure into an ultrasound signal, and our #OxyLite tied mechanism to outcome by tracking dissolved oxygen in real time.
The technique may open safer, more sensitive #cardiology pressure monitoring.

#BiomedicalEngineering #Nanobubbles

Not All Tumors Are Alike: Varying Efficacy of FLASH Across Tumor Types and Oxygenation Status in Spheroid Models - PubMed This preclinical study suggests that tumor iso-efficacy with UHDR may not hold true for all cancer types and is associated with oxygen level.

What if FLASH response is tuned by oxygen and spheroid size?

In 3D models, UHDR matched conventional dose rates in ambient oxygen for small spheroids, but hypoxia increased the dose needed for control by about 7 to 14 percent.

#HypoxyLab maintained 8 mmHg O2 to model hypoxia.

#FLASH #Hypoxia

EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers - Nature Communications EXO1 performs multiple roles in DNA replication and DNA damage repair (DDR), but its role in DDR-deficient cancers remains unclear. Here, the authors find EXO1 loss as synthetic lethal with many DDR genes involved in various cancers, including genes from Fanconi Anaemia pathway, BRCA1-A complex, and spliceosome factor ZRSR2; such interactions represent potential clinical targets.

What if blocking EXO1 could selectively kill FA/BRCA1-A/ZRSR2-deficient tumors?

New @nature.com study maps EXO1 synthetic-lethal partners and combo wins (PARPi, cisplatin, IR). Clonogenic survival imaged and analyzed with #GelCount across key figures (2-4).

#DNARepair #CancerResearch #Oncology

Effects of tempol on renal medullary tissue hypoxia in an ovine model of Gram‐negative septic acute kidney injury Renal arterial infusion of tempol (RAT) at the onset of Gram-negative sepsis can prevent sepsis-induced medullary tissue hypoxia and acute kidney injury (AKI). However, it is not known whether treatm....

What if the clock is the biggest variable in septic #AKI?
In an ovine model, tempol begun at 24–31 h did not reverse medullary hypoxia; normalization followed antibiotics.
#OxyLite (pO2) & #OxyFlo (perfusion) revealed cortex-vs-medulla differences in real time.

#Sepsis #TranslationalResearch

Intra-tumoral hypoxia promotes CD8+ T cell dysfunction via chronic activation of integrated stress response transcription factor ATF4 Metabolic stress imposed by the tumor microenvironment (TME) promotes T cell dysfunction and limits antitumor immunity. Alicea Pauneto et al. demonstrate the hypoxia within the TME drives chronic acti...

What if tumor hypoxia is the switch for CD8⁺ T-cell exhaustion?

New Immunity paper shows chronic hypoxia drives ATF4 leading to mitochondrial stress & death. ISRIB restores T-cell fitness and PD-1 response.

The #HypoxyLab was set to 1.5% O₂ for 96 h to model cell stress.

#Hypoxia #Immunotherapy

Effect of flow change on brain injury during an experimental model of differential hypoxaemia in cardiogenic shock supported by extracorporeal membrane oxygenation - Scientific Reports Scientific Reports - Effect of flow change on brain injury during an experimental model of differential hypoxaemia in cardiogenic shock supported by extracorporeal membrane oxygenation

What if a simple flow change could curb ECMO-related brain injury?
In an ovine VA-ECMO DH model, increasing flow to 4.5 L/min boosted PbTO₂ (+215%) and reduced histologic damage vs 2.5 L/min.
Cerebral measurements achieved with #OxyLite (pO₂) + #OxyFlo (laser-Doppler flow).
#ECMO #Neuroprotection

Hi Prof. Garg,

Interesting letter to the editor here.

In most early preclinical and clinical studies using urine pO₂ as a renal-health marker (including those mentioned in your article), teams used our OxyLite to monitor urine O₂ in catheters. If your teams are exploring this, please reach out.

What if flipping CRC colony morphology could boost chemo?

New @nature.com paper shows azithromycin drives re-epithelialization in 3D collagen and sensitizes to irinotecan. Validations used #GelCount to objectively count 3D colonies.

#CancerResearch #3DCulture

www.nature.com/articles/s42...

Keeping incubators and workstations contamination-free Maintain good laboratory practice by keeping CO₂ incubators and hypoxia workstations contamination-free with tools like MycoFog™, for greater confidence in experimental results

We recently partnered with MycoFog to offer a simple-to-use decontamination device for cell incubators and workstations (HypoxyLab included).

Read this article to reduce contamination risk and keep experiments on track:

www.oxford-optronix.com/resources/ke...

#LabSafety #CellCulture #CleanLab

Contributions of DNA Double Strand Break Repair Pathways to DNA Crosslink Repair DNA crosslink-inducing drugs are widely used in clinical settings for treatment of solid tumors. Double strand breaks (DSBs) that arise during interst…

What if ICL repair isn’t one pathway’s job?

DNA Repair (2025): NHEJ, TMEJ & HR co-contribute to crosslink repair, with dependencies shifting when NHEJ is impaired. Survival readouts (Fig. 1, 3, 4) were supported by #GelCount to image and analyze the clonogenic assays.

#DNARepair #GenomeStability

Frontiers | Astrocytes from lamina cribrosa are involved in the autoregulatory function of optic nerve head vessels in vitro PurposeTo investigate the role of the lamina cribrosa (LC) astrocytes in the autoregulatory capacity of optic nerve head (ONH) vessels and to explore the und...

What if #astrocytes steer optic nerve head blood flow?

Study shows OGD/R → mTOR activation in LC astrocytes, ↑PGE₂, and relaxed VSMCs, supporting astrocyte-driven autoregulation. Hypoxia verified with OxyLite (<10 mmHg after 1 h).
#OxyLite often lets teams fine-tune media pO₂ to in-vivo levels.

UPR-induced intracellular C5aR1 promotes adaptation to the hypoxic tumour microenvironment - Cell Death & Disease Cell Death & Disease - UPR-induced intracellular C5aR1 promotes adaptation to the hypoxic tumour microenvironment

What if we’ve been aiming at the wrong surface?

Under <0.1% O₂, UPR drives intracellular C5aR1 that supports tumour cell survival — favouring cell-permeable inhibitors. #GelCount used to track 3D spheroid growth and to image/analyze clonogenic plates.

#Hypoxia #Spheroids #ClonogenicAssay

Hypoxia ameliorates neurodegeneration and movement disorder in a mouse model of Parkinson’s disease - Nature Neuroscience Parkinson’s disease (PD) involves toxic protein buildup and energy failure in neurons. Continuously breathing low-oxygen air protected mice from PD-like neuronal loss and reversed symptoms, even after...

What if less oxygen could protect the Parkinson’s brain? 🧠
Using Oxford Optronix’s #OxyLite sensor, researchers found α-syn aggregation causes brain hyperoxia—& that hypoxia reversed it, restoring motor function.
#Neurodegeneration #ParkinsonsResearch

Read here: www.nature.com/articles/s41...

Intramuscular adrenaline to improve outcomes following cardiac arrest – An experimental porcine study To investigate early intramuscular adrenaline as an add-on treatment for cardiac arrest in a clinically relevant porcine model. We hypothesized that i…

Can early IM adrenaline save more lives after cardiac arrest?
Early IM adrenaline in cardiac arrest boosted adrenaline levels & heart output but didn’t improve ROSC or brain O₂. Brain oxygen monitoring with #OxyLite showed the gap between systemic gains & brain delivery.
#Resuscitation #Neuro #Brain

Rapid depletion and super-resolution microscopy reveal dual roles of SRSF5 in coordinating nuclear speckle–paraspeckle crosstalk during cellular stress Abstract. Nuclear speckles (NS) and paraspeckles (PS) are adjacent yet distinct nuclear condensates that undergo stress-induced reorganization. Here, we id

What does hypoxia do to your RNA?

A new Nucleic Acids Research paper reveals that low oxygen impairs translation & reshapes RNA mods.
They used the HypoxyLab for precise O₂ control at 0.2% to compare to normoxic conditions.

Read here: academic.oup.com/nar/article/...

#Hypoxia #RNA #LabTech

YouTube video by Oxford Optronix Ltd., UK GelCount 2 intro video

Meet GelCount 2—the new benchmark in automated colony, spheroid & organoid analysis:
⚡ 50% faster imaging
📊 Quantitative size and count data
🧪 Trusted by researchers worldwide with 500+ citations
#CancerResearch #DrugDiscovery #Organoid #LabAutomation

Watch Here: www.youtube.com/watch?v=vYou...

Object Classification in Mammalian Cell Colony, Spheroid, and Organoid Counting Explores how automated imaging and analysis tools define inclusion and exclusion criteria in cell colony, spheroid, and organoid analysis. This article covers how systems like GelCount automate the de...

Are you really counting colonies, or just noise?
GelCount's multi-parameter object classification removes the guesswork—enabling clean, reproducible counts of colonies, spheroids, and organoids.
#Colony #Organoid #Spheroid #LabAutomation #GelCount #CancerResearch

Head-to-head comparison of V-A ECMO, Impella and ECPELLA in normal ovine hearts - Scientific Reports Scientific Reports - Head-to-head comparison of V-A ECMO, Impella and ECPELLA in normal ovine hearts

Study in Scientific Reports compares ECMO, Impella, and ECPELLA in healthy sheep hearts.

Real-time #OxyLite pO₂ tracking enabled precise MVO₂ calculations across devices.

🧪 A foundational study for future cardiomyopathy models.

#CardiacSupport #Physiology #HeartResearch #AHA #ECMO

Recruitment of renal functional reserve by intravenous amino acid loading in a sheep model of cardiopulmonary bypass - Intensive Care Medicine Experimental Background Cardiopulmonary bypass (CPB) may decrease the renal functional reserve (RFR). However, the temporal changes in RFR after during the recovery period after CPB remains unknown. We assessed RF...

How does mechanical ventilation affect kidney oxygenation in sepsis?
This study tracked PO₂ in the renal medulla using OxyLite fiber-optic probes (OxyLite) and found a ~25% drop under mechanical ventilation.

Read here➡️ link.springer.com/article/10.1...

#Sepsis #Renal #Kidney #TranslationalScience

YouTube video by Oxford Optronix Ltd., UK HypoxyLab product intro

Just dropped: a 2-min explainer on the #HypoxyLab — a sealed, mmHg-controlled hypoxia chamber used in cancer, stem cell, and neuroscience research. Come see our novel approach to an oxygen controlled workstation!

and yes, there’s a blooper at the end 👀

#hypoxia #researchtools #science #labtech

First in vitro and in vivo experiments with ultra high-dose rate oxygen ion radiotherapy Within this study we demonstrated the feasibility of ultra-high dose rate (UHDR) oxygen ion irradiation at three different levels of biological comple…

A novel publication data using #OxyLite in vitro:
- Oxygen consumption drops with increasing LETd (0.35→0.18 mmHg/Gy)
- UHDR always lowers O₂ uptake than SDR
Implication: heavy‑ion FLASH may protect normal cells while effectively hitting tumors
#FLASH #RadiationOncology #Tumor

An episodic burst of massive genomic rearrangements and the origin of non-marine annelids - Nature Ecology & Evolution An analysis of annelid genomes reveals massive reshuffling of chromosomes in the ancestral lineage leading to clitellates, a clade composed of non-marine annelids, with potential implications for the ...

How to evolve from ocean to soil? A genome explosion!
New Nature study reveals clitellates underwent massive chromosomal scrambling—shattering ancestral gene order.
The #HypoxyLab was key: its precise O₂ control validated #adaptive responses.
#Evolution #Ecology

🔗: www.nature.com/articles/s41...

Targeting BATF2-RGS2 axis reduces T-cell exhaustion and restores anti-tumor immunity - Molecular Cancer Objective This study aims to investigate the role of RGS2 in immune regulation in lung cancer (LC) and explore the regulatory relationship between RGS2 and BATF2 in modulating T cell exhaustion and tu...

New lung cancer research: BATF2-RGS2 axis drives T-cell exhaustion, limiting immunotherapy. RGS2↑ creates poor PD-1 response.

#GelCount automated colony counting validated 3D colonies (Supp Mat 3), enabling unbiased size/count analysis.

#Immunotherapy #LungCancer #CancerResearch #LabAutomation

BPS Publications Background and Purpose Pulmonary hypertension (PH) results from pulmonary vasculopathy, initially leading to a compensatory right ventricular (RV) hypertrophy, and eventually to RV failure. Hypoxia ...

ITPP prevents RV failure in PH by addressing hypoxia.
Using #OxyLite sensors, researchers found RV pO₂ dropped to 15 mmHg in PH rats (vs. 31.5 mmHg controls). ITPP raised pO₂ to 22 mmHg, reducing mortality.
Proof that oxygen delivery can rescue a failing heart.
#Cardiology #PulmonaryHypertension

Brainstem depolarization–induced lethal apnea associated with gain-of-function SCN1AL263V is prevented by sodium channel blockade An infant presenting with life-threatening apneic events was found to carry a homozygous SCN1AL263V missense mutation, which caused familial hemiplegic migraine in heterozygous family members. We show...

@pnas.org study: A SCN1A mutation causes lethal apnea by triggering brainstem depolarization.
Using the #OxyLite , researchers tracked pO2, revealing medullary hypoxia occurs after depolarization—showing apnea drives hypoxia.
Key insights for SIDS and sudden death research.
#Apnea #Neuroscience

Hypoxia-inducible factor 1α modulates acrolein-induced cellular damage in bronchial epithelial cells Acrolein, a highly reactive α,β-unsaturated aldehyde, is a widespread environmental pollutant. It is generated during the incomplete combustion of mat…

HIF-1α’s dual role in acrolein lung damage revealed by a @baylor.bsky.social team!
Study shows hypoxia via #HypoxyLab lets HIF-1α either worsen or protect cells, depending on exposure. Key for understanding smoke/pollution toxicity! #Hypoxia #LungHealth

www.sciencedirect.com/science/arti...

Interplay between ALK2R206H mutant receptor and autophagy signaling regulates receptor stability and its chondrogenic functions - Cell Death Discovery Cell Death Discovery - Interplay between ALK2R206H mutant receptor and autophagy signaling regulates receptor stability and its chondrogenic functions

New @nature.com study: Hypoxia drives colorectal cancer stemness & drug resistance. Our HypoxyLab provided precise low-O2 conditions, confirming ↑ stemness markers (OCT4/NANOG) & chemo resistance. Vital for modeling tumor microenvironments.

#CancerResearch #Hypoxia #CRC #StemCell #Research