Tyler Inskeep (they/them)

🔥my latest paper from @odedrechavi.bsky.social lab🔥
we found small RNAs act across tissues to regulate fertlity in C. elegans 🪱. Surprisingly, we also found that O2-sensing neurons inhibit germline maintenance. Follow along our journey👇https://www.biorxiv.org/content/10.1101/2025.08.07.669182v1

Solanum pan-genetics reveals paralogues as contingencies in crop engineering - Nature Gene duplication and subsequent paralogue diversification are major obstacles to genotype-to-phenotype predictability.

🚨New paper published in @nature.com! Using pan-genetics across the Solanum genus🍅🥔🍆we reveal why gene duplications🧬are major contingencies in crop engineering. My postdoc work in the Lippman lab @CSHL, collab. with @katiejenike.bsky.social @mikeschatz.bsky.social chatz.bsky.social and many others!

Network properties constrain natural selection on gene expression in Caenorhabditis elegans Gene regulatory networks (GRNs) integrate genetic and environmental signals to coordinate complex phenotypes and evolve through a balance of selection and drift. Using publicly available datasets from...

this project began in the winter while plants and (root-knot) nematodes were bulking and experiments had to slow - thanks again to niels for supporting me in exploring new ideas and venturing out of my comfort zone!

www.biorxiv.org/content/10.1...

would love to hear your thoughts! ⬇️

we believe our findings are largely consistent with macroevolutionary theories of transcriptome evolution - this suggests that gene regulatory networks might evolve in similar ways across time scales

key takeaways

⚖️ selection is mostly weak - drift dominates!

🕸️ core genes face stronger stabilizing selection (but trans-regulatory variation can relax this)

🧠 tissue-specific genes are under stronger selection - with neuronal and reproductive tissues evolving the fastest

what we did:

🧬 we used genotypic selection analyses to link variation in transcript levels to fecundity to test how natural selection shapes transcriptomes within a single generation

🕸️ we then integrated our models with network models to explore what constrains/drives transcriptome evolution

Network properties constrain natural selection on gene expression in Caenorhabditis elegans Gene regulatory networks (GRNs) integrate genetic and environmental signals to coordinate complex phenotypes and evolve through a balance of selection and drift. Using publicly available datasets from...

🚨 excited to share that my first, first author manuscript is now accessible on biorxiv!

in it, my mentor @simoncgroen.bsky.social and I explore how gene expression evolves in real time in C. elegans, leveraging an incredible dataset from Erik Andersen’s group!

www.biorxiv.org/content/10.1...