Karl Carlström

How dopamine producing neurons arise in the developing brain In a new study from Karolinska Institutet, published in Nature Neuroscience, researchers have identified the neurogenic progenitor that give rise to dopaminergic neurons, the primary neurons affected ...

KI researchers have identified the neurogenic progenitor that gives rise to dopaminergic neurons – the primary neurons affected in #Parkinsons disease and essential for controlling movement. The study is published in @natneuro.nature.com 🧪 #neuroscience news.ki.se/how-dopamine...

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis: @cellpress.bsky.social www.cell.com/cell/fulltex...

3 papers in Cell @cellpress.bsky.social today exploring the mechanistic connection between Epstein-Barr virus and multiple sclerosis #MultipleSclerosis
cell.com/cell/fulltex...
cell.com/cell/fulltex...
www.cell.com/cell/fulltex...

TGFβ signaling mediates microglial resilience to spatiotemporally restricted myelin degeneration - Nature Neuroscience Zhu et al. find that aging causes region-specific myelin damage in the spinal cord, which is counteracted by enhanced TGFβ signaling in microglia, revealing a protective mechanism for healthy aging.

Microglia checkpoints promote healthy myelin aging
Our new paper is out in Nature Neuroscience @natneuro.nature.com

www.nature.com/articles/s41...
🧵1/3

With all the excitement about AI giving us answers, we often forget that the real trick is to figure out what is the question.

There’s an art to writing a grant proposal so that your creativity is on full display. Maria Leptin - president of the ERC - talks about it on the new episode of the Night Science Podcast.

First paper from the lab is now online
@natneuro.nature.com !
We mapped injury induced enhancers in the mouse CNS and decoded their sequence architecture. Little 🧵 rdcu.be/eSQi1

Major new direction in the lab: hacking human cell biology with pathogen effectors (eORFs) - amazing collaboration with @miketilapia.bsky.social lab. Huge congrats to first authors Tomas & He & all co-authors! Check out the pre-print on @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...

Generating long deletions across the genome with pooled paired prime editing screens Engineered deletions are a powerful probe for studying genome architecture, function, and regulation. Yet, the lack of effective methods to create them in large numbers and at multi-kilobase scale has...

New 🧬✂️ pre-print! We show that paired prime editing can efficiently generate large deletions — even >1 Mb — with high precision and at scale. We use this to perform the first pooled prime deletion screen across the human genome.

🔗 biorxiv.org/content/10.1...

A short thread (by Juliane Weller)👇

We are looking for a Research Assistant with an interest in functional metabolic and epigenetic interplay in oligodendrocytes! To study this we apply a wide range of leading-edge technologies… | Kar... We are looking for a Research Assistant with an interest in functional metabolic and epigenetic interplay in oligodendrocytes! To study this we apply a wide range of leading-edge technologies, inclu...

We are hiring!!
Interested in applying various CRISPR editing approaches and cell profiling to address functional metabolic and epigenetic interplay in oligodendrocytes?!

Apply here 👇
www.linkedin.com/posts/karl-c...

Oligodendrocytes show early changes in multiple sclerosis A new study from Karolinska Institutet, published in Nature Neuroscience, explores how oligodendrocytes, the cells that produce myelin in the brain and spinal cord, respond during the development of m...

A new study in @natneuro.nature.com by @gocastelobranco.bsky.social et al explores how oligodendrocytes respond in #MultipleSclerosis. The researchers identified distinct and early disease-related states and gene activity patterns that may help explain how #MS progresses 🧪 news.ki.se/oligodendroc...

Distinct transcriptomic and epigenomic responses of mature oligodendrocytes during disease progression in a mouse model of multiple sclerosis - Nature Neuroscience Single-cell ATAC/RNA multiomic profiling was used to investigate how oligodendrocyte lineage cells respond across multiple disease stages within a mouse MS model, revealing early activation of immune ...

Our paper, driven by Chao Zheng and Bastien Hervé in the lab @ki.se, is now out in @natneuro.nature.com!
Check it out at:
www.nature.com/articles/s41...

with @pkukanja.bsky.social, @deckardw.bsky.social, among others

Interestingly we identified MS-associated SNPs to regulate oligodendrocyte proliferation and differentiation but also cytokine secretion!!
Great joint effort with @deckardw.bsky.social and remaining twitterless authors! Enjoy!

New preprint from
@gocastelobranco.bsky.social lab!! We use MPRA & scCRISPRi/a to interrogate possible functions of GWAS-identified Multiple sclerosis risk SNPs in iPSC-derived oligodendrocytes.
www.biorxiv.org/content/10.1...

Chromatin fatigue: DNA repair alters the chromatin environment and introduces heritable variation in gene expression in a larger region around the lesion! Amazing achievement by @sbantele.bsky.social and Jiri Lukas published in @science.org 🙌 Happy we could contribute. See 👇

Researchers at KI and @yale.edu have created a multidimensional, molecular map of how the mouse #brain develops after birth and how it reacts to #inflammation. The study is published in @nature.com. 🧪 #neuroscience news.ki.se/scientists-m...

Scientists map how the brain develops – and how it resolves inflammation Researchers at Karolinska Institutet and Yale University have created a multidimensional, molecular map of how the mouse brain develops after birth and how it reacts to inflammation. The study, which ...

Researchers @ki.se and Yale University have created a multidimensional, molecular map of how the mouse #brain develops after birth and how it reacts to #inflammation #research supported by Knut and Alice Wallenberg Foundation #MS #neuroscience
@gocastelobranco.bsky.social
news.ki.se/scientists-m...

Latest from the lab in colab with @rongfan8.bsky.social

Låter som något äkta gnällisar skulle säga.

🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...

Our study on neuroblastoma developmental plasticity is the cover story in the latest issue of Dev. Cell @cp-devcell.bsky.social .The Chinese-ink Taiji (Yin–Yang) cover to echo cell-state transitions. Huge thanks to the editors, co-authors. #Neuroblastoma www.cell.com/developmenta...

A few weeks ago, I had to discuss about DynaTag, a smart tweak to CUT&Tag that enables single-cell transcription factor mapping with just a buffer change.
Opening a number of future avenues.
📰 Via
@GenomeWeb
: lnkd.in/djYm_sGg
#epigenetics #singlecell

Min vän Jovan har startat cykelverkstad i Gubbängens centrum cykelnomad.com. Blev en räddad palm som inflyttningspresent. Vi jobbade tillsammans för 10 år sedan och han har jobbat heltid som cykelmekaniker sen dess. Sprid gärna ordet så han kommer igång ordentligt!

David Baltimore, Nobel-Winning Molecular Biologist, Dies at 87

Of David's countless accomplishments is the important work that Matt Porteus did in his lab on early-stage genome editing with engineered nucleases (pubmed 12730593).
He and Matt both speak about this in the documentary "Human Nature."
A true titan.
www.nytimes.com/2025/09/07/s...

Balancing off-target and on-target considerations for optimized Cas9 CRISPR knockout library design The continued development of high-dimensional CRISPR screen readouts, such as single-cell RNA sequencing and high-content imaging, necessitates compact libraries to enable functional interrogation at ...

Doomscrolling pause to chat CRISPR libraries! Preprint describes our new, data-driven approach to combine on-target and off-target predictions much more intelligently for *selecting* guides, which we use to develop our newest Cas9 knockout library, Jacquere. Thread: www.biorxiv.org/content/10.1...

Metformin alters mitochondria-related metabolism and enhances human oligodendrocyte function Nature Communications - Metformin is under clinical trial for brain neuroprotection but how it may work is poorly understood. Here, the authors show that metformin helps myelin formation by human...

Our new paper on metformin, myelin, mitochondria and metabolism is now out at rdcu.be/eDbFD
Thanks to Nina and all of the team!

A careful, comprehensive and creative characterization of T cell dynamics in response to pathogens, with all credits going to @rohamparsa.bsky.social who just opened his lab!

Excited to share our new preprint! We studied how T cells are functionally & clonally shaped across organs over time. With the new TRACK mice, we genetically label recently activated T cells with tdTomato and track their migration across tissues. #immunology #science www.biorxiv.org/content/10.1...

Cecilia Uddén: ”Som journalist ska du vara opartisk – men objektiv kan ingen vara” Cecilia Uddén har hamnat i fokus för debatten om journalistik och opartiskhet.

Träffade SR:s Mellanösternkorrespondent Cecilia Uddén och pratade om Gaza, opartisk journalistik och sorgen efter hennes kärlek Elisabet Ohlson.