bioRxiv Synthetic Biology

Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells https://www.biorxiv.org/content/10.64898/2025.12.11.693673v1

Innovative Prevention Approach for Pan Allergens-Induced Allergy: Effective Regulation of Heat Shock Protein 70 and Profilin 3 by siRNA-Loaded Nanomaterial https://www.biorxiv.org/content/10.64898/2025.12.09.693215v1

Going beyond size: Exploring the metabolic burden inPseudomonas putida during heterologous proteinproduction https://www.biorxiv.org/content/10.64898/2025.12.09.693165v1

Structure-based design of antibody repertoires with drug-like properties https://www.biorxiv.org/content/10.64898/2025.12.10.693474v1

A transcription factor-responsive enhancer discovery platform for targeted immunotherapy https://www.biorxiv.org/content/10.64898/2025.12.08.693012v1

Engineering a Covalent Linkage into a Dimeric De Novo Enzyme Reveals a Novel Life-Sustaining Mechanism https://www.biorxiv.org/content/10.64898/2025.12.09.693225v1

Structure and evolution-guided design of minimal RNA-guided nucleases https://www.biorxiv.org/content/10.64898/2025.12.08.692503v1

Conjugation Based CRISPR Antifungals (COBRA) https://www.biorxiv.org/content/10.64898/2025.12.06.692781v1

Elucidation and engineering of arabinofuranosyltransferase to enable total de novo biosynthesis of paris saponins in yeast https://www.biorxiv.org/content/10.64898/2025.12.07.692791v1

Generative design and construction of functional plasmids with a DNA language model https://www.biorxiv.org/content/10.64898/2025.12.06.692736v1

A genetic platform for a biocementation bacterium https://www.biorxiv.org/content/10.64898/2025.12.05.692644v1

Design of post-translational, ligand-controlled inverters and switches https://www.biorxiv.org/content/10.64898/2025.12.05.692672v1

Repurposing the diatom periplastidial compartment for heterologous terpenoid production https://www.biorxiv.org/content/10.64898/2025.12.05.692597v1

Biochemical Upcycling of PET via Glycolysis and Engineered Microbial Consortia https://www.biorxiv.org/content/10.64898/2025.12.04.692332v1

Stimuli-Expressed Protein Qubits for Quantum Quantification of Heavy Metals in Natural Water https://www.biorxiv.org/content/10.64898/2025.12.04.692285v1

Synthetic Modular Cyanobacterial Consortium Enables Carbon-Negative Production of Glycerol and Derivatives https://www.biorxiv.org/content/10.64898/2025.12.04.692262v1

Design and synthesis of mirror image fluorescent nucleotide reversible terminator, (L)-3'-O-azidomethyl-dGTP-N3-fluorophore, and (D)-9°N mutant DNA polymerase for mirror image (L)-DNA sequencing by synthesis https://www.biorxiv.org/content/10.64898/2025.12.03.692211v1

Rapid, Growth Factor-Reduced Differentiation of Functional Neurons from hiPSCs https://www.biorxiv.org/content/10.64898/2025.12.03.692194v1

A foundation model for microbial growth dynamics https://www.biorxiv.org/content/10.64898/2025.12.01.691707v1

Design principles of neuromorphic computing using genetic circuits https://www.biorxiv.org/content/10.64898/2025.12.01.691482v1

Full-length structure of CPR-containing self-sufficient cytochrome P450 https://www.biorxiv.org/content/10.1101/2025.11.29.689641v1

Generative inverse design of RNA structure and function with gRNAde https://www.biorxiv.org/content/10.1101/2025.11.29.691298v1

An ultra-low background far-red light-responsive optogenetic tool based on an engineered biliverdin-binding domain https://www.biorxiv.org/content/10.1101/2025.11.29.691301v1

Engineering Clostridium thermocellum for production of 2,3-butanediol from cellulose https://www.biorxiv.org/content/10.1101/2025.11.28.691234v1

Optimization of heuristic logic synthesis by iteratively reducing circuit substructures using a database of optimal implementations https://www.biorxiv.org/content/10.1101/2025.11.28.691216v1

Engineered symbiont biosensor maps micron-scale sugar gradients in the honeybee gut https://www.biorxiv.org/content/10.1101/2025.11.28.691108v1

Iterative and modular expression of Botryococcus braunii genes enhances isoprenoid production in the diatom Phaeodactylum tricornutum https://www.biorxiv.org/content/10.1101/2025.11.27.690994v1

Paradoxical gene regulation explained by competition for genomic sites https://www.biorxiv.org/content/10.1101/2025.11.27.691022v1

PhenoDEL as a Novel Screening Strategy Based on Intracellular Protein Degradation Activity https://www.biorxiv.org/content/10.1101/2025.11.26.690606v1

Energy-guided combinatorial co-optimization of antibody affinity and stability https://www.biorxiv.org/content/10.1101/2025.11.26.690765v1