bioRxiv Synthetic Biology

Bioluminescent sentinel plants enable autonomous diagnostics of viral infections https://www.biorxiv.org/content/10.1101/2025.08.05.668616v1

Simulating a for-loop in the human genome: Design and evaluation of recombinase genetic programs that count to three. https://www.biorxiv.org/content/10.1101/2025.08.04.668540v1

Bistability in Gene Regulation: Simulating Positive Feedback and Toggle Circuits Using Python and Hill Functions https://www.biorxiv.org/content/10.1101/2025.08.05.668746v1

Epistatic mutations in ISC metabolism synergize with cell cycle regulation and the PPP to enhance xylose fermentation and acetic acid tolerance in industrial yeast https://www.biorxiv.org/content/10.1101/2025.08.04.668411v1

Rewiring amino acid metabolism to boost nonnatural polyketide production in Yarrowia lipolytica https://www.biorxiv.org/content/10.1101/2025.08.03.668335v1

A Sequence Motif Enables Widespread Use of Non-Canonical Redox Cofactors in Natural Enzymes https://www.biorxiv.org/content/10.1101/2025.08.01.668186v1

Sequence mismatch between gene-drive and target-site flanking regions significantly impairs homing efficiency in Culex quinquefasciatus https://www.biorxiv.org/content/10.1101/2025.08.01.668174v1

Design-driven optimization of low-cost reagent formulations for reproducible and high-yielding cell-free gene expression https://www.biorxiv.org/content/10.1101/2025.08.01.668204v1

InSillyClo, a user-friendly web application to assist large-scale Golden Gate Cloning and MoClo workflows https://www.biorxiv.org/content/10.1101/2025.07.31.667881v1

Activation-Free Upgrading of Carboxylic Acids to Aldehydes and Alcohols https://www.biorxiv.org/content/10.1101/2025.07.28.667276v1

Self-assembling protein materials with genetically programmable morphology and size https://www.biorxiv.org/content/10.1101/2025.07.24.666636v1

Design and Characterisation of a Sensor Integrating Chemical and Temperature Inputs for Gene Expression https://www.biorxiv.org/content/10.1101/2025.07.24.666488v1

Metabolic engineering approach to boost γ-decalactone production in S. cerevisiae identifies a critical role for the peroxisomal Fatty Acyl-CoA Synthetase Faa2 https://www.biorxiv.org/content/10.1101/2025.07.21.665937v1

Screening and machine-learning assisted prediction of translation-enhancing peptides reducing ribosomal stalling in Escherichia coli https://www.biorxiv.org/content/10.1101/2025.07.17.665026v1

Breaking the Salinity-Nitrogen Fixation Trade-Off: Engineering A Synthetic Nitrogen-Fixing Vibrio natriegens Strain https://www.biorxiv.org/content/10.1101/2025.07.16.665069v1

Harnessing Dynamic Cell Responses to Optimise Pluripotency Protocols https://www.biorxiv.org/content/10.1101/2025.07.17.665308v1

Synthetic Aptamer Mechanoreceptors Enable Cell-SpecificForce Sensing and Temporal Control via DNA Circuits https://www.biorxiv.org/content/10.1101/2025.07.16.665263v1

Engineering fluoroacetate dehalogenase by growth-based selections to degrade non-natural organofluorides https://www.biorxiv.org/content/10.1101/2025.07.16.665192v1

CF2H: a Cell-Free Two-Hybrid platform for rapid protein binder screening https://www.biorxiv.org/content/10.1101/2025.07.16.665152v1

Stimuli-driven cyclical content exchange in a composite synthetic cell https://www.biorxiv.org/content/10.1101/2025.07.16.665112v1

Simple high-throughput encoding of deep mutational scanning libraries by oligo-based Golden Gate assembly https://www.biorxiv.org/content/10.1101/2025.07.16.665225v1

Design-Build-Test-Learn guided engineering of a whole-cell pyruvate biosensor based on transcription factor https://www.biorxiv.org/content/10.1101/2025.07.14.664704v1

Engineering Microbial R- and S- β-Hydroxybutyrate Production https://www.biorxiv.org/content/10.1101/2025.07.15.664879v1

Modulating Cardiac-Gut Microbiome Interaction Post-Myocardial Infarction with Engineered Bacteria https://www.biorxiv.org/content/10.1101/2025.07.10.664025v1

Comparing LigandMPNN and Directed Evolution for Altering the Effector-Binding Site in the RamR Transcription Factor https://www.biorxiv.org/content/10.1101/2025.07.10.663684v1

Rewiring DNA Repair Activity into CRISPR Signal Transduction via Synthetic DNA Transducers https://www.biorxiv.org/content/10.1101/2025.07.11.664310v1

Light-up nanostructures with allosteric DNA mimics of GFP https://www.biorxiv.org/content/10.1101/2025.07.10.664110v1

Metalorian: De Novo Generation of Heavy Metal-Binding Peptides with Classifier-Guided Diffusion Sampling https://www.biorxiv.org/content/10.1101/2025.07.10.664242v1

The Coli Toolkit (CTK): An extension of the modular Yeast Toolkit for use in E. coli https://www.biorxiv.org/content/10.1101/2025.07.09.663901v1

A coupled fluorescence assay for high-throughput screening of polyurethane-degrading enzymes https://www.biorxiv.org/content/10.1101/2025.07.09.663332v1