Kay Schink

Interview with Sandra Iden – President of the German Society for Cell Biology ABSTRACT. Sandra Iden is Professor and Chair of Cell and Developmental Biology at Saarland University in Homburg, Germany. Her research focuses on cell–cell communication in epithelial tissue homeosta...

Recent interview for @jcellsci.bsky.social. Thx for important services JCS & @biologists.bsky.social provide to the cell science community. Glad we could share aspects on #50yearDGZ @dgz.bsky.social, @dfg.de @hetcci.bsky.social SPP2493, #CGBM & my great team @uni-saarland.de

shorturl.at/rCQg5

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues.
www.biorxiv.org/content/10.1...

Plasma membrane labelling efficiency, internalization and partitioning of functionalized fluorescent lipids as a function of lipid structure Labeling the plasma membrane for advanced imaging remains a significant challenge. For time-lapse live cell imaging, probe internalization and photobleaching are major limitations affecting most membr...

New paper out! Would you like to fluorescently label the plasma membrane in live, fixed or permeabilized cells? I tested different lipid structures and labelling approaches to decide what makes a good membrane labelling probe for such imaging experiments!
www.biorxiv.org/content/10.1...

Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...

Amazing story, André. Congratulations!

Quantitative imaging of lipid transport in mammalian cells Nature - Directional, non-vesicular lipid transport is responsible for fast, species-selective lipid sorting into organelle membranes.

big one! a decade in the making. nadler & team develop tools to track lipid transport in living cells at single-species resolution. turns out non-vesicular, asymmetry-driven transport is the main architect of organelle identity. feels like the dawn of a golden era for lipid biology. rdcu.be/eBGJv

Have you published lipid-protein interactome data? Please let us know, we’d love to include it in the repository. The goal is to build a centralized hub for the scientific community.

Huge thanks to Gaelen Guzman, a graduate student/postdoc in the lab who built it from scratch.

Postdoctoral Research Fellowship in Chromatin Biology and Cancer Epigenetics (283381) | University of Oslo Job title: Postdoctoral Research Fellowship in Chromatin Biology and Cancer Epigenetics (283381), Employer: University of Oslo, Deadline: Wednesday, August 20, 2025

We are hiring! A 3-year postdoc in #Chromatin #Cancer #Epigenetics at University of Oslo, Norway and co-supervised by @amathelier.bsky.social You will be part of @cancelluio.bosky.social. We focus on #Sarcoma #Epigenome #CRISPR and Drug screen studies. Find out more and apply here: shorturl.at/DtLHM

Group Leader (282908) | University of Oslo Job title: Group Leader (282908), Employer: University of Oslo, Deadline: Monday, September 8, 2025

🚨 We're hiring! NCMBM @ncmbm.bsky.social is looking for 2 new group leaders to join our vibrant research community. Ready to build your own group with strong support and a solid startup package?

Interested and attending #ISMBECCB2025? Let’s connect!

🔗 www.jobbnorge.no/en/available...

The power of basic optical design knowledge and CAD!

-> Here's a fast laser point scanner that connects to a commercial microscope. The design, build and alignment are relatively simple. Much harder is agile software control that integrates with the existing system... Custom hardware is hard!

Intracellular mechanics and organelle mechanobiology Mechanobiology is an interdisciplinary field that emerges at the cross-section of biology, physics and engineering. It aims to understand how living cells, tissues and animals sense and respond to me…

Announcing a new @embo.org Workshop on "Intracellular mechanics and organelle mechanobiology" that we are organizing with Michael Krieg and Verena Ruprecht at @icfo.eu (Barcelona) on 16-20 February, 2026. Please, repost and spread the word! 🙏 #EMBOmechanobio

meetings.embo.org/event/26-org...

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

Fig. 4 Super-resolution microscopy in live and fixed cells with Rhobin. (A) Rhobin transiently binds rhodamine molecules and thereby constantly replenishes signal lost through photobleaching of dyes. (B-D) No-wash STED microscopy of live U2OS cells transiently expressing LifeAct-Rhobin2 labeled with 100 nM JFX650. (C, D) Zoom-ins of regions highlighted in (B) imaged either with confocal or STED microscopy. Scale bar: 20 μm (B) and 10 μm (C, D). (E-H) Rhobin enables timelapse STED microscopy of fast ER dynamics with minimal signal loss. U2OS stably expressing N-terminal tag fusions of SEC61B were stained with 1 μM Halo-JFX650 (HaloTag7, reHaloF) or 2 μM SiRhP (Rhobin2) and imaged at a frame rate of 0.387 fps for at least 100 time points. (F, G) Selected frames from timelapse acquisitions of Rhobin2:SEC61B labeled with SiRhP (F) or HaloTag7:SEC61B labeled with Halo-JFX650 (G). Scale bar: 2 μm. (H) Signal .CC-BY-NC 4.0 International licenseavailable under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprintthis version posted June 25, 2025.;https://doi.org/10.1101/2025.06.24.661379doi:bioRxiv preprint 22 loss during timelapse imaging. Mean ± SD signal inside cells over time for indicated constructs. (I-K) PAINT-type super-resolution microscopy with Rhobin. Rhobin2-SEC61B was transiently expressed in U2OS cells and labeled with 5 nM SiRhP after chemical fixation. Imaging under no- wash conditions and near-TIRF illumination. (I) Repeated, but transient binding of SiRhP molecules to Rhobin2 at low nanomolar concentrations can be observed as intensity bursts in intensity time traces extracted from a diffraction-limited area. See movie S3 for raw data of binding-induced blinking. (J) Sum image across 10,000 frames of an image stack, simulating a diffraction-limited image. (K). Reconstructed image from localized molecules in the full stack.

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...

🔎 Looking to start a PhD in Sept 2025?
The Crick Institute spring round for PhD recruitment is open!
If you are interested in actin cytoskeleton and imaging 🔬 then check out my project on role of Arp2/3 iso-complexes in regulating invadopodia👇please RT

www.crick.ac.uk/careers-stud...

Peroxisomal core structures segregate diverse metabolic pathways - Nature Communications Peroxisomes contain detergent resistant core structures. Bäcker et al. show that these core structures contain diverse enzymes and serve to enable metabolic compartmentalization of the peroxisome lume...

nature.com/articles/s41467-025-57053-9
Finally this work is out - we show that short sequence motifs direct proteins to specific peroxisomal sub-domains. Fantastic collaboration with @bangebalcony.bsky.social , Johannes Freitag @unimarburg.bsky.social and many more! @uio.no @cancelluio.bsky.social

Very proud to be part of that team - this was a really long term project with lots of amazing people!

Peroxisomal core structures segregate diverse metabolic pathways - Nature Communications Peroxisomes contain detergent resistant core structures. Bäcker et al. show that these core structures contain diverse enzymes and serve to enable metabolic compartmentalization of the peroxisome lume...

www.nature.com/articles/s41... Great Joint Venture with Johannes Freitag @synmikro.bsky.social @unimarburg.bsky.social

Fast Antibiotic resistance-Based gene editing of mammalian cells with CRISPR-Cas9 (FAB-CRISPR) Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency causing low knock-in rates. We ...

Long in the making but our protocol for gene editing is online! FAB CRISPR is super easy and allows the generation of N and C terminal fusions with TurboID, SNAP, Halo and mStayGold. A single cloninf step to generate HDR templates! arxiv.org/abs/2502.12675 1/n

Genetically encoded mechano-sensors with versatile readouts and compact size Mechanical forces are critical for virtually all fundamental biological processes, yet quantification of mechanical forces at the molecular scale in vivo remains challenging. Here, we present a new st...

Molecular force measurements in vivo are notoriously difficult. We present a new class of programmable force sensors based on engineered coiled-coils, with modular readouts, including fluorescence and luminescence. doi.org/10.1101/2025...

Welcome to the official Bluesky page of the Institut Pasteur!

🔬 Explore with us the frontiers of biomedical research

🧪 Follow us to uncover groundbreaking discoveries

💡 Share knowledge for global health

🌍 Join a community passionate about progress and open science.

Pasteur is on bluesky!

Genetically Encoded Fluorogenic DNA Aptamers for Imaging Metabolite in Living Cells Genetically encoded fluorescent protein and fluorogenic RNA sensors are indispensable tools for imaging biomolecules in cells. To expand the toolboxes and improve the generalizability and stability of...

Genetically Encoded Fluorogenic DNA Aptamers for Imaging Metabolite in Living Cells
pubs.acs.org/doi/10.1021/...

Very nice

How it started How it's going

have ppl found any funders that support toolmaking? I want to keep making tools for people, but there is a seemingly irresistible force pushing us towards hypothesis-driven research. In my experience, funders don't want to hear about tech dev. Any insights? for both neuro & climate would be great tx

"Till you have sequence confirmation, you are not cloning but clowning." 🧬⭕🤡
- Lab Saying.

#PILife #AlwaysBeCloning

YouTube video by Brian Northan Jupyter Notebook 3D Projections

I finally got around to making a video about my utilities to render 3D projections in Jupyter notebooks. The code is useful but a bit rough so I also suggest taking a look at @guiwitz.bsky.social microfilm and @haesleinhuepf.bsky.social stackview as more polished options.
youtu.be/9yOA8R03trE

Congratulations, Volker! Very well earned!

Pretty happy about this side view of mitochondria in an U2OS.

Pretty good axial resolution for an OPM, and a lightsheet in general.

#Microscopy #LightSheet
#Snouty

go.bsky.app/JDAwbAJ Trying again with the actual link this time. Please tag yourself or others who study macropinocytosis, phagocytosis or efferocytosis, and I’ll add you to the pack!