Allegretti Lab

Dear all, we are looking for a research assistant in protein biochemistry / cell biology to support our work on nuclear reshaping in human cells. Here the link to the call, please spread: mrc.tal.net/vx/mobile-0/...

Using #cryoET to investigate plant cell-cell communication 🤩🌿

Structure of the virulence-associated Neisseria meningitidis filamentous bacteriophage MDAphi

Out in @pnas.org

Congrats @janboehning.bsky.social @abultarafder.bsky.social Miles Graham @mathieucoureuil.bsky.social
@mrclmb.bsky.social @hopitalnecker.bsky.social

www.pnas.org/doi/10.1073/...

A picture of 3 rows of people on steps at the MRC LMB. From left to right - top row: Akaash, Vic, Lara - Middle row: Kerrie, Manu, Paddy - bottom row: Meg and Julie

🚨 JOB ALERT 🚨
We're hiring a Research Assistant – no PhD needed!
Join us to explore the molecular mechanisms of receptor sorting in endosomes.
Experience in protein expression/purification, cloning & cell culture desired 🧫🔬
Apply now 👉 www.nature.com/naturecareer...

#STEMCareers #ResearchJobs

Portrait photo of Tanmay Bharat

Congratulations to @tbharat-lab.bsky.social, Group Leader in the LMB’s Structural Studies Division, who has received the Gold Medal from @embo.org for his research on prokaryotic surface molecules.

Read more here: www2.mrc-lmb.cam.ac.uk/tanmay-bhara...

#LMBNews

Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme Nature Chemistry - Models of abiotic RNA replication suffer from inherent product inhibition arising from the high stability of RNA duplexes. Now, it has been shown that RNA trinucleotide...

New paper from my group rdcu.be/eoaRr "Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme" in Nature Chemistry — great collaboration with attwaterlab.uk.

“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬

“Join the Pigino Lab! We’re hiring a PhD student to explore primary cilia biology using advanced imaging, spatial ‘omics, and AI. Apply now at www.cilia-ai.eu and be part of the Cilia-AI Doctoral Network!”

Finally in peer-reviewed form: Mapping the ultrastructural topology of the corynebacterial cell surface. @bupbuse.bsky.social @vikramalva.bsky.social

Please also see concurrent beautiful work from @adriasogues.bsky.social and Han Remaut.

Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.

www.biorxiv.org/content/10.1...

#cryoEM #transcription #splicing

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

Active microtubule-actin cross-talk mediated by a nesprin-2G–kinesin complex Nesprin-2 Giant links the nucleus to the cytoskeleton, enabling actin transport and nuclear movement through kinesin-1 activation.

science.org/doi/10.1126/... Just published! We uncovered a molecular machine that connects the cell's two major structural networks revealing how cells accomplish precise internal reorganization - a process disrupted in diseases ranging from cancer to multiple sclerosis.

Huge congratulations and best of luck with the new adventure Sven!

Research Group Leader Tenure Track - Structural Studies - LMB 2549 - Cambridgeshire job with MRC Laboratory of Molecular Biology | 12835196 Research Group Leader Tenure Track Starting Salary £65,940 to £74,448 per annum MRC Laboratory of Molecular Biology, Cambridge, UK

We have an exciting opportunity for a new independent group leader with an interest in structural/molecular biology and machine learning to join our Division of Structural Studies at the @mrclmb.bsky.social! 🥳
Re-posts appreciated.

www.nature.com/naturecareer...

The work also explains how this mechanism is selective for cancer cells and not healthy cells!! Enjoy the read! @a-dsantos.bsky.social, @piotrkolata.bsky.social, @mrclmb.bsky.social

Ever wondered how the chemotherapeutic drug paclitaxel can target slow-dividing tumours? Check out our preprint Hale et al! @tom-hale.bsky.social found a novel non-mitotic mechanism of action for paclitaxel involving nuclear-cytoskeletal coupling via the LINC complex!
www.biorxiv.org/content/10.1...

The work also explains how this mechanism is selective for cancer cells and not healthy cells!! Enjoy the read! @a-dsantos.bsky.social, @piotrkolata.bsky.social, @mrclmb.bsky.social

Protein production for cryoEM structure determination just got a lot cheaper - high resolution structure from half a plate of HEK cells with the "MIcro ISolation (MISO)" microfluidics-based approach. Congrats to the Brunner and Efremov labs in Brussels! www.biorxiv.org/content/10.1...

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍

Preprint📜: www.biorxiv.org/content/10.1...

A short thread🧵👇

Twenty-seven scientists become EMBO Young Investigators – Press releases – EMBO The group leaders join an international network of nearly 800 life scientists and receive financial support

Wonderful to see that our colleague @marta-shahbazi.bsky.social is now an EMBO YIP. www.embo.org/press-releas...

Crosslinks can sometimes improve AlphaFold3 models. Crosslinking is an experimental method often used as a source of distance restraints in modeling. In AlphaFold3, instead of using restraints, crosslinks can be included as covalent ligands. Thanks to Agnieszka Obarska-Kosinska for the idea.

Marta Shahbazi, sitting in front of a lab bench

Congratulations to @marta-shahbazi.bsky.social, Group Leader in @cellbiol-mrclmb.bsky.social, who has joined the EMBO Young Investigator Programme!
Read more here about the programme and Marta’s research here: www2.mrc-lmb.cam.ac.uk/marta-shahba...
#LMBNews

MRC LMB

🌟 Welcome to the MRC Laboratory of Molecular Biology on Bluesky!
We’re thrilled to connect with you and share our research insights, job opportunities, events, and more.
Join us on this journey as we advance science and improve human health together!
#MolecularBiology #Research #Innovation

Ok, trying to bring my community back together... I created a starter pack for lipids and membrane protein aficionados!

Feel free to share, add and distribute, so we can come together again! 😍

go.bsky.app/AEeXh86

Here is a starter pack for CryoEM/ET enthousiasts
To be completed
go.bsky.app/JmQksC

Join us! Ill present some of our recent tomo work on sperm cells!

Ever wondered if nuclear pore architecture and function changes during germ cell differentiation? In human spermatogenesis it does, and radically!!
Check out our first lab preprint @AliaCSantos et al! www.biorxiv.org/content/10.1...

Over 4 years in the making, I am extremely happy to share our pre-print on the “In-cell structure and snapshots of copia retrotransposons in intact tissue”. We used cryo-ET to resolve the copia capsid structure to subnanometer resolution inside cells: www.biorxiv.org/content/10.1...

Join us @berkeleyMCB Mar. 25 for Cell Biology at the Nanoscale with @cellarchlab @bronwynalucas.bsky.social @thevillalab.bsky.social and more. Register at tinyurl.com/5n7jd7mr.

The Barad lab is hiring across all levels. Open positions here: baradlab.com/join/. If you are interested in doing cutting edge tomography and computational methods development to study intracellular bacterial infection, and living in Portland, OR, but don't fit the openings, reach out anyway!