Allegretti Lab

Very sad news, John Gurdon has died.

A developmental biologist's developmental biologist, Nobel prize winner

His work is the foundation of much of today's dev & stem cell bio.

An inspiration to many, including me. Always asking questions & wanting the answers

www.magd.cam.ac.uk/news/profess...

Thank you Kanishka for this piece! @tom-hale.bsky.social ky.social, @a-dsantos.bsky.social y.social , @mrclmb.bsky.social

Paclitaxel compromises nuclear integrity in interphase through SUN2-mediated cytoskeletal coupling - preLights Paclitaxel destabilizes nuclear integrity in interphase by driving microtubule–vimentin aggregates towards the nucleus, leading to SUN2 dependent loss of Lamin A/C loss and enhanced cancer cell vulner...

Another preprint highlight was posted by Kanishka Parashar!

Read how paclitaxel can target cancer cells by destabilising nuclear integrity in interphase - #preprint from @matteoall.bsky.social & team #preprint

#preLight ⬇️👀
prelights.biologists.com/highlights/p...

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.

www.nature.com/articles/s41...

Many congratulations!! Really great work!

We’re looking for a Research Support Officer to join @matteoall.bsky.social’s group, working with @piotrkolata.bsky.social to investigate nuclear-cytoskeletal coupling in mammalian cells using #cryoEM, #cryoET & other biochemical approaches.
www.jobs.ac.uk/job/DOE317/r...
Closes 31 AUG
#ScienceJobs

Also check out the work of @tzviyazbm.bsky.social on the same topic!

It was a lot of fun and a tremendous synergy of intents dear @rezamehdipour.bsky.social! Thank you

We discovered that the SUN5-Nesprin3 LINC complex forms a hexagonal lattice at the sperm HTCA, which radically reshapes the nuclear membrane locally!
Our findings also provide the structural basis for understanding the severe ASS (Acephalic Sperm Syndrome) male infertility phenotype.

This work is the result of a fantastic collaboration with@matteoall.bsky.social!
@warred.bsky.social, @a-dsantos.bsky.social , @alisterburt.bsky.social , @tom-hale.bsky.social, @mrclmb.bsky.social,

We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!!
www.biorxiv.org/content/10.1...

In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells In eukaryotic cells, LINC complexes physically bridge the two nuclear membranes to cytoskeletal filaments, transmitting mechanical forces to the nucleus. Due to their dynamic and membrane-embedded nat...

Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo

www.biorxiv.org/content/10.1...

Dear all, we are looking for a research assistant in protein biochemistry / cell biology to support our work on nuclear reshaping in human cells. Here the link to the call, please spread: mrc.tal.net/vx/mobile-0/...

Using #cryoET to investigate plant cell-cell communication 🤩🌿

Structure of the virulence-associated Neisseria meningitidis filamentous bacteriophage MDAphi

Out in @pnas.org

Congrats @janboehning.bsky.social @abultarafder.bsky.social Miles Graham @mathieucoureuil.bsky.social
@mrclmb.bsky.social @hopitalnecker.bsky.social

www.pnas.org/doi/10.1073/...

A picture of 3 rows of people on steps at the MRC LMB. From left to right - top row: Akaash, Vic, Lara - Middle row: Kerrie, Manu, Paddy - bottom row: Meg and Julie

🚨 JOB ALERT 🚨
We're hiring a Research Assistant – no PhD needed!
Join us to explore the molecular mechanisms of receptor sorting in endosomes.
Experience in protein expression/purification, cloning & cell culture desired 🧫🔬
Apply now 👉 www.nature.com/naturecareer...

#STEMCareers #ResearchJobs

Portrait photo of Tanmay Bharat

Congratulations to @tbharat-lab.bsky.social, Group Leader in the LMB’s Structural Studies Division, who has received the Gold Medal from @embo.org for his research on prokaryotic surface molecules.

Read more here: www2.mrc-lmb.cam.ac.uk/tanmay-bhara...

#LMBNews

Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme Nature Chemistry - Models of abiotic RNA replication suffer from inherent product inhibition arising from the high stability of RNA duplexes. Now, it has been shown that RNA trinucleotide...

New paper from my group rdcu.be/eoaRr "Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme" in Nature Chemistry — great collaboration with attwaterlab.uk.

“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬

“Join the Pigino Lab! We’re hiring a PhD student to explore primary cilia biology using advanced imaging, spatial ‘omics, and AI. Apply now at www.cilia-ai.eu and be part of the Cilia-AI Doctoral Network!”

Finally in peer-reviewed form: Mapping the ultrastructural topology of the corynebacterial cell surface. @bupbuse.bsky.social @vikramalva.bsky.social

Please also see concurrent beautiful work from @adriasogues.bsky.social and Han Remaut.

Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.

www.biorxiv.org/content/10.1...

#cryoEM #transcription #splicing

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

Active microtubule-actin cross-talk mediated by a nesprin-2G–kinesin complex Nesprin-2 Giant links the nucleus to the cytoskeleton, enabling actin transport and nuclear movement through kinesin-1 activation.

science.org/doi/10.1126/... Just published! We uncovered a molecular machine that connects the cell's two major structural networks revealing how cells accomplish precise internal reorganization - a process disrupted in diseases ranging from cancer to multiple sclerosis.

Huge congratulations and best of luck with the new adventure Sven!

Research Group Leader Tenure Track - Structural Studies - LMB 2549 - Cambridgeshire job with MRC Laboratory of Molecular Biology | 12835196 Research Group Leader Tenure Track Starting Salary £65,940 to £74,448 per annum MRC Laboratory of Molecular Biology, Cambridge, UK

We have an exciting opportunity for a new independent group leader with an interest in structural/molecular biology and machine learning to join our Division of Structural Studies at the @mrclmb.bsky.social! 🥳
Re-posts appreciated.

www.nature.com/naturecareer...

The work also explains how this mechanism is selective for cancer cells and not healthy cells!! Enjoy the read! @a-dsantos.bsky.social, @piotrkolata.bsky.social, @mrclmb.bsky.social

Ever wondered how the chemotherapeutic drug paclitaxel can target slow-dividing tumours? Check out our preprint Hale et al! @tom-hale.bsky.social found a novel non-mitotic mechanism of action for paclitaxel involving nuclear-cytoskeletal coupling via the LINC complex!
www.biorxiv.org/content/10.1...

The work also explains how this mechanism is selective for cancer cells and not healthy cells!! Enjoy the read! @a-dsantos.bsky.social, @piotrkolata.bsky.social, @mrclmb.bsky.social

Protein production for cryoEM structure determination just got a lot cheaper - high resolution structure from half a plate of HEK cells with the "MIcro ISolation (MISO)" microfluidics-based approach. Congrats to the Brunner and Efremov labs in Brussels! www.biorxiv.org/content/10.1...