We hope this dataset serves as a resource for dissecting and eventually targeting T cell dysfunction in cancer and chronic infection. Extremely grateful to everyone who contributed, including collaborators @santoshvardhana.bsky.social Klebanoff Lab, Abdel-Wahab lab, Ari Hakimi, and Jiankun Lyu lab

Cysteine reactivity profiling together with functional follow up studies highlight signaling and stress pathways linked to this metabolic insufficiency, including MAPK/JNK, p53/DNA damage, ER stress, proteasome regulation, and other proteome-wide effects.

These analyses reveal extensive metabolic rewiring and bioenergetic stress in chronically stimulated T cells, including dysfunction of de novo purine and pyrimidine synthesis.

Here we present an integrated multi-omic characterization of human T cell dysfunction, combining cysteine-reactivity-based chemical proteomics with proteomic, transcriptomic, and metabolomic profiling to track how molecular programs evolve during chronic TCR stimulation.

Chronic antigen stimulation progressively impairs T cell self-renewal and cytotoxicity, but the molecular basis of this transition is still poorly understood, especially at the post-translational level.

Excited to share our new preprint: “A post-translational regulatory map of chronic antigen-driven human T cell dysfunction.”! www.biorxiv.org/content/10.6...

We leverage this platform to study energy balance-dependent remodeling of secreted and ER-resident proteins. We hope this tool and the resulting datasets will be useful for the community for dissecting cell-specific contributions to systemic metabolic regulation.

Excited to share our new preprint in collaboration with Paul Cohen's lab at Rockefeller University and Ken Loh's lab at Yale University! We introduce TurboID-TMT for cell type-specific proximity labeling to profile organ secretomes and ER-resident proteomes in vivo. www.biorxiv.org/content/10.6...

Faculty recruitment - Our Scientists

We are hiring! www.rockefeller.edu/our-scientis...

Very excited to join #CZBiohubNY as part of the recent cohort of new investigators!

Applications for the 2025 Bioorganic GRC are now live! Here is the link where you can check out the current line-up:

grc.org/bioorganic-c...

Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes Many human proteins lack chemical probes and are even considered undruggable. Ogasawara et al. describe diversity-oriented synthesis-inspired photoreactive small molecules (photo-stereoprobes) and the...

Cool paper from Cravatt lab: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes: www.cell.com/cell-chemica...

We further leverage advanced chemical proteomic workflows to explore more broadly the largely untapped potential for targeting of splicing with cysteine-reactive covalent electrophiles.

Reposting here! Check out the final version of our manuscript now in @cellchembiol.bsky.social ! In this study, we investigate the mechanism of action of an immunosuppressive small-molecule electrophile, which targets a core splicing factor SF3B1! @rockefelleruniv.bsky.social

Hello, BlueSky! I'm excited to be here! Thank you, @carolynbertozzi.bsky.social for helping me with the move!