Hannes Maib

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

📣Paper alert! Interested in faster and brighter protein labeling than before? 👀 @steffi-k.bsky.social & other colleagues have developed an improved tool for labeling proteins with synthetic fluorophores for bioimaging. Also works in STED microscopy 🤩🔬 Congratulations!🥳
www.nature.com/articles/s41...

Shit.

In all my interactions with @jacoates.bsky.social I can vouch for his sincere commitment and drive towards open science and engagement. Moving on from his role with ASAPbio will be a great opportunity for whomever he will work with next!

A graphic for the Marie Skłodowska-Curie Actions (MSCA), showing a historical portrait of Marie Skłodowska-Curie overlaid with an image of four young researchers walking down a hallway. The European Commission logo is in the top left. Text reads: "Marie Skłodowska-Curie Actions – €404.3 million to support postdoctoral researchers”

Choose Science. Choose Europe.

A new Marie Skłodowska-Curie Actions Postdoctoral Fellowships 2025 call is now open.

With a budget of €404.3 million, it will support around 1,650 researchers from Europe and beyond.

Apply by 10 September → europa.eu/!fBTMgF

I’d prefer one meeting a day. Having one meeting freshens up my mind and gets me thinking, which also helps with writing. Many meetings a day drag me down and exhaust me, which is also worse for the other person in the meeting as they won’t be able to get the best out of me

Looking for a student or postdoc!

Want to combine evolutionary analysis + structural modeling with in vitro biochemistry & fluorescence microscopy?
Help us uncover new protein-protein interactions — from in silico to in vitro.

Join us at @istaresearch.bsky.social 👉 looselab.org
🧬💡🔬🎡

If using Bloomigton #Drosophila Stock Center stocks, pls. acknowledge them & their NIH funding (P40 OD018537). Papers listing this no. are being harvested as evidence. We massively depend on the @bdsc.bsky.social & they need our support in these dire times! @flybase.bsky.social @fly-eds.bsky.social

Pls re-skeet! There's a postdoc job available to join us in Bristol! We're looking for a molecular cell biologist to help us investigate plasma membrane proteostasis in neurons. Apply here by 23 April: www.jobs.ac.uk/job/DMO097/r.... If interested, do contact me and check out grieve-lab.com! Thanks!

Only 10 days left to apply!

We are looking for a passionate protein biochemist for the reconstitution of lipid kinases. We have long term funding and you will be trained in the use of supported lipid bilayers.

If you are interested just get in touch or apply directly!

First time playing around with single molecule STORM imaging of PI3P on endosomes using recombinant biosensors. This was great fun with our new fellow Ruby Peters and I see lots of potential for this in the future!

It is the scourge of our times and as a foreigner in the UK it does not feel any better. It is clear that the spirit of open borders and free movement is coming to an end but Germany is still as strong part of the EU. And at least in Germany there is clear and vocal opposition to the far right

Research Associate in Reconstitution of Lipid Kinases at University of Sheffield An opportunity for an academic position as a Research Associate in Reconstitution of Lipid Kinases is available, as advertised on jobs.ac.uk. Apply now and explore other academic job openings.

🚨Long term postdoc position available on the reconstitution of lipid kinases!🚨

Do you have a passion for protein reconstitution and lipid signalling? You will reconstitute lipid kinases from eukaryotic expression systems to determine their kinetic regulation.

www.jobs.ac.uk/job/DLW981/r...

Science is Under Siege: The Open Science Community Must Act and Lead by Example Worldwide, science is under attack by anti-scientific movements. The new US administration has thrown the entire academy into chaos, threatening global health. This article is a call for the open scie...

The open science community must lead by example in defending science.

We are ideally positioned to combat the efforts by Trump, Musk & others that are anti-science & anti-inclusion.

Time to practice what we preach!

#academicsky #openscience #metasci #ScholComm

Cell polarity and membrane dynamics The ability of cells to polarize is essential for organismal development and physiology, and its impairment underlies numerous diseases. The establishment and maintenance of cell polarity requires a …

Registration is open for the 2025 iteration of the wonderful @EMBOevents workshop on cell polarity and membrane dynamics #EMBOcellPolarity25 I am organizing with Ed Munro! Wonderful lineup wonderful please please sign up and share! meetings.embo.org/event/25-cel...

Incredibly honoured to have my first paper as PI highlighted in this collection! The idea was do a quick tool paper as my start into independence and it turned out to be much more useful than I thought. Read about how to use recombinant biosensors to stain for lipids in fixed cells!

I want to share one of my favourite #microscopy images with you all:
Epithelial cells in a monolayer (NMuMG) after undergoing EMT stained for N-Cadherin and actin show beautifully interdigitated cell junctions. Bonus points for spotting the actin asters ⭐

Clathrin-coated pits, plaques, and adhesion... This post is dedicated to Christien Merrifield, who left us far too soon around this time in 2017. His memory continues to inspire 🔬
doi.org/10.1016/j.js...

Apply to become a reporter at the Biologists @ 100 conference! - the Node Keen to get more science communication experience? Is your research related to developmental and stem cell biology? The Node is looking for a reporter to attend and report from the Biologists @ 100 co...

*Last few days to apply!* We're looking for a #DevBio researcher interested in gaining #SciComm experience to join us at @biologists.bsky.social Biologists @ 100 conference.

We're open to any ideas💡If selected, we'll work with you to develop your ideas and assist you at the conference.
More info👇

#Preprints allowed @maiblab.bsky.social
✍️ disseminate research to the community
🗯️ get valuable information about how well it is received
💡 highlight to journals that there is a strong interest in Hannes' work
📄 made it easier to get past the editor's desk

Trying this out for #fluorescencefriday. 🧪 AktPH through an isosurface mesh of the plasma membrane. From this lattice lightsheet collaboration with @minesbioimaging.bsky.social: www.nature.com/articles/s41...

Congratulations to Meiyan Jin on her beautiful publication out today. www.sciencedirect.com/science/arti...

Great opportunity for postdocs interested in molecular neuroscience/ biochemistry to study presynapse formation in human neurons funded by the ERC (see www.leibniz-fmp.de/haucke; Rizalar et al Haucke (2023) Science). We are a certified green lab. Apply now to haucke@fmp-berlin.de before 5 Dec 2024.

EndoConnect Symposium - Program The EndoConnect Symposium on Endo-Lysosomes in Metabolic Regulation Find below the final program and the book of abstracts for the inaugural “EndoConnect Symposium on Endo-Lysosomes in Metabolic...

On my way to brave the snow to get to the EndoConnect conference (endoconnect.eu/endoconnect-...) in Cambridge now. Really looking forward to three days of endo/lysosomal sorting.

Follow me for more fun in phosphoinositide biology, using a combination of in vitro reconstitution and cell biology!

Now in my independent lab we are working on a range of different phosphoinositide conversions. From PI(5)P to PI(4,5P2 conversion during peroxisome membrane contact site formation to the PI(3)P to PI conversion by the Myotubularins during receptor trafficking.

By segmenting the beads we can quantify these differences and show that active Arf6 strongly enhances the reaction speed of phosphoinositide conversion by the PIP5K. This has drastic effect on membrane trafficking pathways! www.cell.com/current-biol...

Importantly it also allows us to directly compare beads with different surface functionalisation. Here I attached the constitutively active mutant of the small GTPase Arf6 (Arf6-QL) and the inactive mutant to beads and we see complete separate reaction kinetics!

These realtime kinetics allow us to monitor individual reactions and revealed strong stochastic effects due to complex feedback loops as has been shown by Scott Hansen and Jay Groves www.pnas.org/doi/full/10....

In my first real post I want to show the key experiment that revealed to me what I want my own lab to be about:
The conversion of phosphoinositides!
By using fluorescent biosensors and membrane coated beads I was able to reconstitute the real time kinetics of PI(4)P to PI(4,5)P2 conversion by PIP5K

I prefer the old, reliable horror over the new flashy thing that will leave me hanging in the middle of a presentation in front of everyone!