Julie Carnesecchi

Systematic analysis of noncanonical ribosomal protein paralogs does not provide evidence for specialized functions in Drosophila

So excited to finally be able to share my first paper and the culmination of my PhD work with @felipekteixeira.bsky.social 🪰🔬

www.pnas.org/doi/10.1073/...

Congrats!!

When setting goals, we often focus on achieving milestones like a PhD, job, tenure, or promotion. Those are great goals but they’re not enough.

We also need to think about:
-Why we’re pursuing that goal
-What specifically we need to do to succeed
-The values we will follow as we do the work

RNA and Proteins: Who Bosses Whom?

Despair seems like a reasonable course of action
'Some estimates are that up to 20% of the entire proteome can bind RNA and moreover that about 20% of all known protein complexes have an RNA component in them and you really have to think about how to deal with such large figures.'

A reminder that the abundance of 'housekeeping' proteins varies significantly across cells and tissues.

GAPDH is often slandered as a 'housekeeping' protein, and its abundance varies significantly across human tissues.

How do you define a 'housekeeping' protein ?

Congrats @longweibai.bsky.social and al 🥳🥳

Hi folks,

Check out our updated website!
Details on next year's conference will be posted there soon!

fly-jedi.org

Cold induced #RNA binding protein linked with longevity and DNA repair

Excited to share my first PhD student’s @biorxivpreprint.bsky.social!

Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!

Check it out!

www.biorxiv.org/content/10.1...

AI-generated image of a lightsaber-wielding figure conquering Portugal.

Preparations for the next JEDI meeting are underway! The upcoming Junior European #Drosophila Investigator / #NewPI meeting is planned for Portugal in June. Final dates and additional details will be announced soon - stay tuned for updates!​

Two individually marked bumble bees are shown in a tunnel used for affective contagion experiments.

This week in Science, researchers demonstrate positive affective contagion—a core component of empathy—in bumble bees.

The discovery shows that even insects can share affective states, tracing the roots of affect and social cognition deep into evolution. Learn more: https://scim.ag/4oblMQq

Principles of protein abundance regulation across single cells in a mammalian tissue Protein synthesis and clearance are major regulatory steps of gene expression, but their in vivo regulatory roles across the cells comprising complex tissues remains unexplored. Here, we systematicall...

This article is a homecoming for me.

As a PhD student, I focused on the growth-rate transcriptional regulation in yeast.

Now, ~ 20 years later, we report protein regulation scaling with the growth rates of single cells in mammalian tissues.

www.biorxiv.org/content/10.1...

A new family of ribosome hibernation factors in Archaea Ribosome hibernation preserves translation machinery during stress, yet its mechanisms in Archaea remain poorly defined. Here we identify Hib, a previously unrecognized family of archaeal hibernation factors. Genetic, structural and biochemical analyses show that Hib binds ribosomal subunits, blocking the mRNA channel and tRNA sites to inactivate translation. Deletion of hib in Thermococcus barophilus delays recovery from stationary phase and reduces 70S ribosome pools, establishing its role in ribosome preservation. Hib displays a unique modular architecture, combining a bacterial-like HPF module with tandem CBS domains. Cryo-EM structures reveal conformational heterogeneity of Hib:ribosome complexes, consistent with dynamic engagement, and the CBS-containing N-terminal domain binds adenine nucleotides, suggesting a link between hibernation and energy status. These findings define Hib as a key archaeal hibernation factor and provide a framework for understanding ribosome dormancy and adaptation across all domains of life. ### Competing Interest Statement The authors have declared no competing interest. Agence Nationale de la Recherche, https://ror.org/00rbzpz17, ANR-25-CE12-4161

A new family of ribosome hibernation factors in Archaea | bioRxiv https://www.biorxiv.org/content/10.1101/2025.10.11.676729v1?rss=1

M2 interdisciplinary internship RNA, Hox, Evo-Devo | Julie Carnesecchi Funded #M2 #Internship (6 months) — Montpellier | IGMM × ISEM We are recruiting a motivated M2 student to join a joint project on Hox factors beyond DNA, testing how RNA binding by conserved transcri...

@igmm-montpel.bsky.social @isemevol.bsky.social @umontpellier.bsky.social
www.linkedin.com/posts/julie-...

M2 internship (funded, 6months) in Montpellier — IGMM × ISEM with amazing @aigverte.bsky.social
Project: Hox factors beyond DNA: how RNA binding links molecules to body-plan evolution.
Start: early 2026
Apply: CV+motivation
#Master2 #Internship #Montpellier #RNA #Hox #EvoDevo #Drosophila #Bat

Figure from the preprint showing sensory neuron morphology with different levels of Cas9 expression.

High levels of Cas9 are toxic in sensory neurons. Reducing Cas9 levels with uORFs avoids toxicity and is compatible with efficient editing. From @thompsonpeerlab.bsky.social. Fly lines @vdrc-flies.bsky.social

#CRISPR #Drosophila

www.biorxiv.org/content/10.1...

FlyBase Update – October 2025 The termination of the NIH/NHGRI FlyBase grant has placed the long-term sustainability of FlyBase at risk. However, thanks to the generous support of several key individuals and institutions, we are pleased to announce that FlyBase will remain operational through the coming year. We extend our deepest gratitude to Yukiko Yamashita, Cassandra Extavour, Hugo Bellen, Thom Kaufman, the Genetics Society of America / Drosophila Board, the Bloomington Drosophila Stock Center, an anonymous donor and the Wellcome Trust. We are especially thankful for a generous gift from Seemay Chou, Jed McCaleb, and The Navigation Fund. We also greatly appreciate the continued support from the broader Drosophila community – your donations and service fees have been vital in helping us stay afloat. Special thanks also go to Jessica Manning for her tireless administrative work at Harvard, to Ruth Lehmann, Hugo Bellen, and Paul Sternberg for advice and efforts, and to the Board of the European Drosophila Society for all their efforts. Sadly, we must also share that several long-standing FlyBase team members have recently moved on. We are immensely grateful to Susan Russo-Gelbart, Lynn Crosby, Gil dos Santos, Kris Broll, Victoria Jenkins, and TyAnna Lovato for their many years of dedicated service and contributions to FlyBase. Looking ahead, ensuring FlyBase’s sustainability beyond the next year – and successfully integrating with the Alliance – will require new funding sources. We kindly ask for your continued support: • European labs: Please consider contributing to the Cambridge, U.K. FlyBase group • U.S. and other non-European labs: Please consider contributing to the U.S. FlyBase groups • Both U.K. and U.S. FlyBase are working diligently to establish an invoicing system. We appreciate your continued patience. For more information on how to support us, please visit: Contribute to FlyBase wiki page https://wiki.flybase.org/wiki/FlyBase:Contribute_to_FlyBase

There's an update on the state of FlyBase on the FlyBase.org front page. You can contribute to FlyBase at this link wiki.flybase.org/wiki/FlyBase...
We express enormous gratitude to the people, labs, groups, and foundations who have already helped us.
#FlyBase #Drosophila

That’s a wrap! We are done with #EDRC2025 and we look forward to seeing everyone again in 2027 for #EDRC2027 in Bonn!

JEDI folks having dinner at #EDRC2025

Update from #EDRC2025! Four days of amazing talks and poster sessions and JEDIs have reconnected across geographical boundaries. Dinner was fun and we are looking forward to everyone at the next #Drosophila conference!

#edrc2025 European #drosophila Society meeting announces the 2029 meeting location : see you all in Szeged (and Bonn in 2027 of course)!

We are on day 3 of #EDRC2025 continuing with excellent #Drosophila science

Dear JEDIs,
as we are self-organizing using the #EDRC as a nucleator we decided it is time for a JEDI database to boost our network! If you identify as a JEDI, please contact us here or send a mail to katja.rust@uni-marburg.de to be added to our database.
#Drosophila
@fly-eds.bsky.social

Welcome to the second day of #EDRC2025 starting with two great keynote lectures by Cassandra Extavour from Harvard University and Yulong Li from Peking University

3, 2, 1 and we are on!!! #EDRC2025 is starting! Welcome to our more than 740 participants from more than 40 countries! Thank you to all our sponsors!

Welcome to the #EDRC2025 pre-opening workshops! #ECM #Drosophila

Only a few hours left before our meeting starts! Are you travelling to #EDRC2025 now? Let us know by using the oficial #EDRC2025 hashtag!

🧬 NEW Starr-Luxton Lab PREPRINT: Our 🌠grad student
@XiangyiDing
figured out why muscular dystrophy mutations cause such widespread cellular chaos. The answer wasn't what we expected - and it changes how we think about the disease. Thread🧵 (1/6). www.biorxiv.org/content/10.1...

Dear JEDIs, the #EDRC meeting is coming soon! Who is joining? Looking forward to meeting everyone next week!

Coming 👍

We are all super happy and proud to see our work on the function and evolution of the #cephalic #furrow published in @nature.com. Let me say a few things about the background and history of this work on the #Evolution_of_Morphogenesis (1/12)