Ingrid Bunholi

🚨 New paper in Molecular Ecology Resources!
We compiled the most applied rapid DNA/eDNA-based ID tools & primers for #sharks and #rays 🦈
A practical guide to help scientists, managers & enforcement choose the right method for conservation action.
Read here: doi.org/10.1111/1755... #OpenScience #eDNA

I would not miss this opportunity!

Elacatinus phthirophagus is a sister species of E. randalli and is endemic from Brazil. Many species from Brazil also occur in the SE Caribbean - which is actually the rationale behind one of my PhD chapters 🐠🧬

Elacatinus randalli (Bohlke & Robins, 1968)

Main traits: Max 4.6cm; snout yellow/brown with short narrow yellow bar; narrow yellow stripe along upper body (from top eye to tail fin base)

Distribution: SE Caribbean

I actually photographed one during my fieldwork trip in Belize 2023. Not the nicest photo but it was amazing being able to spot such a beautiful and endangered fish!

Epinephelus striatus (Block, 1792)

Nassau grouper (Serranidae)

Main traits: Max 122cm; tan + irregular dark bars; black saddle (mark) tail base; tan stripe Y shape through eyes.
Distribution: Bermuda to Guyana

📷 Mark Betts (STRI website)

Not sure why I chose this one to be the first. I think I like its name 😂

Acanthemblemaria aspera (Longley, 1927) Roughhead blenny (family Chaenopsidae)

Main traits: max 4cm; small pointed spines on snout; colors changeable (male colorless to dark - females yellowish)

Distribution: The Bahamas to the South Caribbean

📷 CABRA lab

One of this year’s goals: get better at identifying fish species 🐠
I’ll be sharing one species a day to keep me on track - hopefully it lasts 😆 If you’re not a fan of fish... well, you’ve been warned.

The MIEM guidelines: Minimum information for reporting of environmental metabarcoding data Environmental DNA (eDNA) and RNA (eRNA) metabarcoding has become a popular tool for assessing biodiversity from environmental samples, but inconsistent documentation of methods, data and metadata make...

Check out our latest paper on #eDNA and metabarcoding data reporting standards 🤓🧬🧪

These minimum reporting guidelines for metabarcoding workflows extend from the physical layout of laboratories through to data archiving enhance trust and reliability in data

mbmg.pensoft.net/article/1286...

Thank you @ednacollab.bsky.social for the opportunity to attend this course! It was intense, but an immensely valuable experience 🧬

Such a fun evening with the 2nd CABRA lab cookie competition 🍪and of course @chemingson.bsky.social won as the most decorative! Who wants to guess which fish species he’s representing? 🐠🤣

Potential Role of Photochemistry in Environmental DNA Degradation Given the severe loss of species richness across diverse ecosystems, there is an urgent need to assess and monitor biodiversity on a global scale. The analysis of environmental DNA (eDNA), referring t...

Are you curious how #eDNA is degraded by photochemistry in the environment? I teamed up with Environmental Chemist's @krismcneill.bsky.social and Eliane Ballmer to see if we could map out what is known and how to move forward --> pubs.acs.org/doi/10.1021/...

Great! Thank you!!

Hi Pedro! How small are you saying? Around 100bp? Also, testing the primer set with regular PCR is enough or would be more appropriate to test with qPCR?

Low‐coverage sequencing and Wahlund effect severely bias estimates of inbreeding, heterozygosity and effective population size in North American wolves vonHoldt et al. ((2024), Molecular Ecology, 33, e17231) (vH24) used low-coverage (average ~ 7X read depth) restriction site-associated DNA sequence data to estimate individual inbreeding and heterozy...

Reposting this from twitter from a few months ago. I think this paper is a must-read for anyone in #PopGen. Always important to consider biases inherent in our methods.

onlinelibrary.wiley.com/doi/full/10....

Can I be added to the list? Thank you!

1st CABRA Lab CryptoVison Fish-ID contest it was definitely one of the best lab meetings of the semester 🐠🐠🐠

New publication led by 🌟 undergrad Ariel Wang: doi.org/10.1007/s003...

We investigate Risor ruber, the tusked goby, a tiny sponge-dwelling fish.

Across the Caribbean, we find seven distinct genetic lineages within Risor and discover that two Risor lineages specialize on a single sponge host!

I started an #eDNA starter pack as one didn’t exist yet. Feel free to message me to get your name added. Open to all! go.bsky.app/U2vgV5Q

Just had a wonderful week in Mexico with the Risor ruber team for our writing (and fun) retreat! It’s been amazing to learn and work with y’all! So excited to what’s to come! #gobyteam 🐠🧬 @TheresaRueger @jordancasey.bsky.social @gobyone.bsky.social

Starting the week with great news! I'm so happy to have been awarded the 2024 @ICRSCoralReefs Graduate Fellowship to conduct part of my doctoral research exploring the connectivity between Brazilian and Caribbean reef fishes! 🐠🧬

Thrilled to share my PhD preliminary findings at the 52nd Benthic Ecology Meeting! I’m so excited to continue unveiling the world of this little cryptobenthic fish! Stay tuned for updates on their pop gen, microbiome and ecological specialization 🧬🐠 #BEM2024

Our paper in the Brazilian press! "Eight out of ten species of sharks and rays in national trade are threatened with extinction" abori.com.br/oceanografia... @ingridbunholi.bsky.social

Excited to finally have this paper out after multiple years in the making.

"Analysing biological colour patterns from digital images: An introduction to the current toolbox"

onlinelibrary.wiley.com/doi/10.1002/...

@biomaralvarenga.bsky.social

Fifteen years of elasmobranchs trade unveiled by DNA tools: Lessons for enhanced monitoring and conservation actions The trade of elasmobranchs (sharks and rays) in Brazil threatens the country's rich endemic biodiversity. The present study explored the use of DNA-ba…

8/8 This research involved collaboration between Brazilian and international institutions 🇧🇷🇵🇹🇺🇸 I am very grateful for the incredible team effort. Find the study here: [link]. Available free for 50 days: www.sciencedirect.com/science/arti...

7/8 Our study highlights research gaps. Address them will improve trade assessments using DNA based-tools and help develop measures for shark and ray protection. Updating legislation, enhancing monitoring, and fostering collaborations are crucial for comprehensive conservation action.

6/8 We examined national and international extinction risk status lists and key conservation and labeling legislation. The SALVE System expanded Brazil's species assessment. Hopefully, it will inform new management legislation, that should align with labeling ones to boost conservation.

5/8 We also found bony fish in trade, alongside other mislabeled samples. Mislabeling, including the generic umbrela-labels (i.e. "cação") obscures species identification, hamper consumer trust and conservation efforts. Standardized labeling is vital for accurate trade regulation.