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Roelfes group - Open Positions PhD position on Hybrid biocatalytic cascades aided by Machine learning (1.0 FTE)

📢📢📢 PhD Opportunity!

Join our research group at RUG! We're seeking a motivated PhD student for a project on biocatalytic cascade processes combining natural and designer enzymes, supported by machine learning.
Click below to learn more — and feel free to share!

sites.google.com/rug.nl/roelf...

Incredible work!! Cheers for the fun-to-read thread!

What an amazing paper!
Using the molecular dye eosin Y as a photosensitizer to generate reducing equivalents in an engineered microbe. If that's not enough, they expressed a rhodopsin proton pump that generates ATP, enhancing the CBB cycle and CO2 conversion to acetoine.
doi.org/10.1016/j.jc...

[2Fe-2S] clusters are responsible for so many amazing biocatalytic conversions and we always rely on their presence in host cells when designing cascades both in vitro and in vivo. Here's an interesting article that shines light on the "birth" of these clusters inside the cells.

Inspiring stuff: using the power of biocondensates for creating distinctive environment in cells for new-to-nature biocatalysis performed by artificial metalloenzymes. Synthetic biology at its best!

By incorporating two cofactors into the four binding sites of streptavidin, we engineered programmable artifical enzymes for tandem abiotic transformations including an enantioselective formal C–H hydroxylation and a photooxidation-Michael addition. onlinelibrary.wiley.com/doi/10.1002/... #chemsky

Artificial Gold Enzymes Using a Genetically Encoded Thiophenol‐Based Noble‐Metal‐Binding Ligand Incorporating noble metals in artificial metalloenzymes (ArMs) is challenging due to the lack of suitable soft coordinating ligands among natural amino acids. We present a new class of ArMs featuring...

Interesting paper by the @roelfesgroup.bsky.social and the group of Daniel Sauer in @angewandtechemie.bsky.social. They used genetic code expansion to incorporate a thiophenol-containing amino acid into a protein and used it to make an artificial gold enzyme. onlinelibrary.wiley.com/doi/10.1002/...