Nick showing Ron Vale the eduWOSM.

22.06.2025 08:02 — 👍 8    🔁 1    💬 0    📌 0

In BIORXIV/2025/651652, we use ATPγS to reveal a previously-occult kinesin state. In the Await-Isomerisation (AI) state, ATP is bound but NL docking and hydrolysis are inhibited. ATPγS overstabilises the AI state, producing a characteristic signature of load-independent 8 nm tethered backslips:

02.05.2025 10:10 — 👍 0    🔁 0    💬 0    📌 0

Nick has been in Rennes, helping Romain Gibeaux install a WOSM-TIRF. Big happiness!

05.04.2025 14:05 — 👍 8    🔁 2    💬 0    📌 0

Tubulin isotypes polymerise into sectioned microtubules that locally regulate protein binding Microtubules assemble from tubulin heterodimers composed of conserved α- and β- tubulins. In many species, including humans, tubulins are expressed from multiple genes. While the resulting tubulin iso...

Prakash et al (doi.org/10.1101/2025...), emerging from a happy & ongoing multilab collaboration, shows that a mix of human α1β3 and α1β4 tubulins assembles in vitro into segmented microtubules in which the segments have different protofilament numbers, and are recognized by different MAPS.

14.03.2025 08:41 — 👍 0    🔁 0    💬 0    📌 0

Yean Ming Chew and I wrote doi.org/10.1042/BST2... about MT lattice switching. We discuss evidence that protofilament-level structural switching is ancient and fundamental and conserved; and that tubulation creates new allosteric interfaces that operate on essentially the same structural switch.

12.02.2025 16:21 — 👍 9    🔁 5    💬 0    📌 1