Tej

Public CPTAC Meeting dctd.cancer.gov/abou...

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#proteomics #prot-conf

From the reviewer's question, it seems to be for a niche application or atleast not conventional proteomics? Either way, access need not be investment limited ..core facilities come in handy here! Recently ours did nichePTM analysis upon client req 'with specific MS' as per their referred literature

Looking for proteomics researchers who want to make a broader impact! Join the US HUPO Education & Outreach Committee to help shape how our field teaches, connects, and grows.

Apply by September: tinyurl.com/ys67uvs4

#Proteomics #USHUPO #SciComm

Discovery and validation of biomarkers for Parkinson’s disease from human cerebrospinal fluid using mass spectrometry-based proteomics analysis These candidate biomarkers add to our understanding of PD pathophysiology and the relationship between PD and DLB. They provide further research directions toward disease-modifying therapies.

Discovery and validation of biomarkers for Parkinson’s disease from human cerebrospinal fluid using mass spectrometry-based proteomics analysis #EBioMedicine #MassSpec www.thelancet.com/journals/ebi...

Great opportunity to pursue labs doing spatial proteomics, allosteric proteomics, proteome dynamics, protein ubiquitinylation, neurodegenerative proteomics..

Post-Translational Modifications Remodel Proteome-Wide Ligandability Post-translational modifications (PTMs) vastly expand the diversity of human proteome, dynamically reshaping protein activity, interactions, and localization in response to environmental, pharmacologi...

Excited to share a new preprint from the lab. We show that PTMs like phosphorylation & glycosylation dynamically reshape proteome-wide ligandability in cells, including proteins like KRAS. Great collaboration with the Huang Lab, @forlilab.bsky.social and BMS. www.biorxiv.org/content/10.1...

#TeamMassSpec Curious on the average recovery observed across labs for SP3 sample prep.. seeing about ~50-60% of input (cell lyates by BCA pep est.) and found the following from recent literature (on FFPE).

pubs.acs.org/doi/10.1021/...

doi.org/10.1016/j.mc...

The ten dollar proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers Mass spectrometry (MS)-based proteomics remains technically demanding and prohibitively expensive for many large-scale or routine applications, with per-sample costs of hundreds of dollars or more. To...

New preprint!
Deep proteome profiling for just $10/sample, all-in.
→ Single-step prep for ≤50 ng
→ 6k–8k proteins in ~10 min
→ No gain from more sample/time
Congrats to Siqi Huang, Chao Wang & Hsien-Jung Lin!
www.biorxiv.org/content/10.1...

Mass Spectrometry Technology Specialist, Międzynarodowy Instytut Biologii Molekularnej i Komórkowej w Warszawie, Warszawa Job offer Mass Spectrometry Technology Specialist, Międzynarodowy Instytut Biologii Molekularnej i Komórkowej w Warszawie, Warszawa

🤩 JOB ALERT 🤩 Come work with me and two cutting-edge LC-MS/MS systems at our brand new Mass Spectrometry Facility @iimcb.bsky.social in Warsaw! Apply here tinyurl.com/y47mzafa or send any questions my way. #TeamMassSpec please share widely!

Agree..my objection was to showing it as a mode of acquisition to compare against their method and synchro PASEF...pydiaid can be used to make even synchro PASEF, narrow-window dia PASEF methods as well..

Yep... this goes against everything on how an article is to be reviewed

"..Despite these advancements, most high-level deep proteomic reports are based on the Orbitrap Astral and Orbitrap Exploris 480, and analytical systems using timsTOF MS still require improvement." And the answer is 100min? Also curious what is py-diaAiD PASEF🤔

Horizon Europe 2028 - 2034: twice bigger, simpler, faster and more impactful Research and innovation news alert: As part of the next long-term EU budget 2028-2034, the Commission is proposing to double the budget of the research and innovation framework programme to €175 billi...

Very promising news on the next seven-year EU budget, with a proposed substantial increase for Horizon Europe including the ERC.

We are analysing the other parts of this proposal in more detail.

@Vonderleyen @EZaharievaEU

research-and-innovation.ec.europa.eu/news/all-res...

It appears the Tenzer lab was well ahead of the curve with this with their platform:
pubs.acs.org/doi/10.1021/...
This was the solution I found to be most useful.

Evaluation of the False Discovery Rate in Library-Free Search by DIA-NN Using In Vitro Human Proteome pubs.acs.org/doi/10....

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#proteomics #prot-paper

Apply today and be part of inspiring science! Deadline July 23rd! #Proteomics #Postdocs

MaxQuant Summer School: July 21–25, 2025 at Utrecht University - NVMS www.nvms.nl/index.ph...

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#proteomics #prot-conf

This is a cool way to look at modified peptides even at proteome scale..as close to manual validation

Thought I came across this article before but apparently it is a different one from Cox lab.. cool to see more articles on understanding peptide conformational changes im TIMS..IM2Deep indeed 😉

bsky.app/profile/past...

▶️▶️ To all mass spec enthusiasts: A postdoc position (three years) is available in our international group. Please apply if you are interested in structural MS and in developing novel cross-link ing MS workflows!🧪
Thanks @diegofgar.bsky.social for sharing!
#teammassspec
#AcademicSky
#ChemSky

Many of you (say, about one million) have used my buffer site to calculate thermodynamically correct buffers. Well, today, I upgraded the site with added security, becuase it was stopping soem people connecting. Should all be fine now. https rather than http. Enjoy!
phbuffers.org/BuffferCalc/...

GitHub - pwilmart/quantitative_proteomics_comparison: Comparison of DIA to spectral counting and TMT quantitative techniques using animal lens studies Comparison of DIA to spectral counting and TMT quantitative techniques using animal lens studies - pwilmart/quantitative_proteomics_comparison

DIA, DOA, DUI, DDA, etc. Here is a comparisons of some quantitative proteomics methods from a POV you might not have seen before:
github.com/pwilmart/qua...

Skeletal muscle proteomics: considerations and opportunities npj Metabolic Health and Disease - Skeletal muscle proteomics: considerations and opportunities

Are you interested in #proteomics and #skeletalmuscle? It can be a real pain to get deep reproducible coverage. Check out our review with @adeshmukh.bsky.social We analyse some considerations including current limitations and how to potentially overcome them #myoblue @unimelbcmr.bsky.social

Diagram of Mascot DIA: Mascot Distiller processes DIA runs into peak lists and detects precursor masses in MS1 and MS2 scans; these are sent to Mascot Server for database searching; finally, Distiller downloads the PSMs and performs MS1 quantitation.

We are working on Mascot DIA, which is a universal spectrum-centric solution for DIA analysis. What is spectrum-centric searching meant to solve? Find out in our blog:

www.matrixscience.com/blog/the-pro...

#proteomics #massspec

It is indeed a flex to show tight CVs ..all the more if it's by hard labour 💪🏾, and would be great to see diy reference video. Albeit cost upfront, automation helps, finished on an analysis that's been at a snail's pace, turns out all I needed was just a day away from the bench and the robot :)

With new age instruments, large files, open searches and large sample nos, this is an important question for cores and labs as well.. quite a few solutions listed in the thread..pick as fot for purpose

‼️ Job alert 🚨: I have an open 3-year postdoc position in my lab to develop novel crosslinking mass spectrometry workflows. 🧪🧪 So if you want to do cool stuff in protein MS, please apply ! ⬇️ Please RT
www.verwaltung.uni-halle.de/dezern3/Auss...

#Academicsky
#Chemsky
#teammassspec

Pumped to finally show off our spatial proteomics work presented a few weeks ago at the EAS Congress @eassociety.bsky.social and now live on @biorxivpreprint.bsky.social 🥳

Time for a science thread! #TeamMassSpec #SpatialProteomics #AtheroSky 🧵

www.biorxiv.org/content/10.1...

News in Proteomics Research blog post | You can fix cells and then ship them out for single cell proteomics! Here are the best ways! proteomicsnews.blogs...

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#proteomics #prot-other

High throughput single-cell proteomics of in vivo cells Single-cell mass spectrometry-based proteomics (SCP) can resolve cellular heterogeneity in complex biological systems and provide a system-level view of the proteome of each cell. Major advancements i...

High throughput single-cell proteomics of in vivo cells #MCP #MassSpec www.mcponline.org/article/S153...