Jim Haseloff

Recipe for PPM (Plant Preservative Mixture) from the patent WO 1996/038542. Simplifies sterile plant tissue culture - especially if you don't have access to a research-grade facility. Great invention from Assaf Guri and Kishor Patel at Plant Cell Technology plantcelltechnology.com/blogs/blog/t...

3D printed rotating tube mixer for incubating samples. Build instructions at: www.printables.com/model/52572-...

Building on my PCB face plate idea, how about a PCB front and back (with the main board in between) and a TPU band round the edge hugging it all in place 🤔 this is just a basic test, but I think it might just work... #Pi0cket #CL32 #ESP32

3D-Printed DIYNAFLUOR Device

DIYNAFLUOR Exploded Filter Cube

DIYNAFLUOR Software GUI

Excited to share the public release of the DIYNAFLUOR (DIY Nucleic Acid FLUORometer), a $40 USD, DIY, open-source, 3D-printed, solder-free, and portable DNA fluorometer you can build. 🦕 🧬

See our GitHub Repo for more! github.com/traulab/DIYN...

#DIYScience #STEM #DIYNAFLUOR

Dear #DIYNAFLUOR users! Please check out the "experimental" folder on our github page.
tinyurl.com/4v23f629

We have a design revision that appears to improve the LoD by ~3.5x, providing quantitation down to 0.16 ng DNA. This should cover v.short nanopore library QC.

Would love any feedback! 🧪🧬🦕❤️

Unlocking the power of Engineering Biology New report outlines the transformative potential of Engineering Biology to tackle modern-day challenges.

Unlocking the power of Engineering Biology www.gov.uk/government/n...

Semi‐automated workflow for high‐throughput Agrobacterium‐mediated plant transformation A simplified and optimized pipeline for Agrobacterium tumefaciens mediated transformation of plants. Useful for obtaining stable transgenic lines of the model liverwort Marchantia polymorpha, or for ...

Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation. Summary of our efforts to accelerate plant transgenics with the @earlhaminst.bsky.social Biofoundry. Now published in @theplantjournal.bsky.social. #PlantScience #Synbio onlinelibrary.wiley.com/doi/10.1111/...

...where a powerful toy slingshot can be used to mount the LoRa node in a tree for better line-of-sight communications with other devices.
www.instructables.com/Meshtastic-S...

Clever description of how to convert an inexpensive solar anchor buoy light into an extremely durable and waterproof solar powered Meshtastic node e.g. for sharing LoRa sensor data in a field setting. www.instructables.com/Meshtastic-S...

1/6 🧵 Excited to share Pocket Spectrometer V2! After positive feedback on the first version of pocket spectrometer. I've made significant improvements (hardware+software) while keeping what worked well with the V1 #OpenSource #Spectrometer #STEM 💡
bsky.app/profile/chin...

chinnadevarapu.bsky.social has updated his tiny, low cost Pocket Spectrometer design that uses an M5StickC microcontroller and AS7341 spectral sensor - simplified hardware connections and software improvements. Nice job! Details at: github.com/scientistnob...

GitHub - scientistnobee/Pocket-Spectrometer: I'm building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with ... I'm building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with AS7341 sensor (~$17.5). My goal? A com...

🚀✨ Exciting innovation alert! Meet the Pocket Spectrometer: a compact, open-source device 📏🔬 (< $50). Affordable, and portable, it is the open-source hardware for spectral analysis 🎨💡. Science in your pocket! 🌟👨‍🔬👩‍🔬 github.com/scientistnob...

The mini capsules can be opened and used for microscopic observation as well.
doi.org/10.1007/978-...

Chopping tubes and adding slits to allow the halves to fit together...
doi.org/10.1007/978-...

Handy little trick from Jose Ignacio Laines-Hidalgo, Angela F. Ku-Gonzalez, and Felipe A. Vazquez-Flota published in doi.org/10.1007/978-...
Making DIY small sealed capsules from Eppendorf tubes for plant histology.

3D print files for a collection of parts that allow micro servos to be plugged into Fischertechnik construction systems. Good for prototyping programmable mechanical devices...
www.thingiverse.com/thing:2807112

The described procedure for making Lenticules is relatively simple. Cells are mixed with a solution containing a high concentration of trehalose, BSA and carboxymethyl cellulose as caking agents, saline (with optional food dye), and dried on parafilm before storage over desiccant.

However, details for preparation of Lenticules appears in a published patent: Storage of microorganisms, cells and tissue (WO 01/05941) See at: link.lens.org/aPFhU0Bjyli

Lenticules were described in doi: 10.1046/j.1365-2672.1998.00611.x. by Codd et al. - but composition of the critical component for storage, the "lenticulating fluid (Codds Medium)" seems missing in this and a subsequent paper (dot: 10.1046/j.1365-2672.2001.01432.x)...

Have always been interested in Lenticules and Vitroids (www.sigmaaldrich.com/deepweb/asse...) - as ways of storing/distributing microbial cells as stable, dry pellets, which could be widely useful for resource sharing. Lenticules originated at Public Health England, and a protocol is available...

Wild collection of heirloom and genetically variant seeds available from Tomato Revolution (www.tomatorevolution.co.uk) ready for next summer.

1/6 🧵 I'm building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with AS7341 sensor (~$16). My goal? A complete spectrometer that is barely larger than a sample cuvette itself.

See how this homemade spectrometer analyzes substances with an Arduino Mega | Arduino Blog Materials, when exposed to light, will reflect or absorb certain portions of the electromagnetic spectrum that can give valuable information about their chemical or physical compositions. Traditional ...

Very cool use of optomechanical elements to make a "scanning" sepctrometer.
blog.arduino.cc/2024/12/23/s...

Downloadable reference card for focus bracketing, on a site full of examples and advice about Olympus macro photography systems from Chris McGinniss
www.chrismcginnis.com/gear

Here's an archived free copy of CL Strong's 1960 book with collected Amateur Scientist columns from Scientific American. Inspiration for citizen science. Forward by Vannevar Bush...
archive.org/details/Proj...

Online portfolio of bryophyte plant images from across the globe - excellent macrophotography from Des Callaghan, captures some of the morphological diversity of this clade
bryophytes.myportfolio.com/work

Fusion 360 files for 3D printing a good-looking Stevenson Screen (en.wikipedia.org/wiki/Stevens...) to house outdoor sensors and electronics, some OpenGreenEnergy. Nice design with other potential applications...
hackaday.io/project/1650...

Successive updates/instructions for building solar-powered environmental sensing stations with custom electronics, wireless communications and 3D printed outdoor housing.
hackaday.io/project/1650... and hackaday.io/project/1820...

Neatly designed $300 EDGE electrophoresis unit with integrated power supply and visualisation system from Edvotek - looks good for education and DIY lab - and design looks extendable
www.edvotek.com/500

Collection of tips and techniques for handling delicate bryophyte plants for microscopy, including sectioning, KOH clearing, slide mounting, etc. from the Britsh Bryological Society
www.britishbryologicalsociety.org.uk/learning/tip...