Nicolas Bigot

📣 16th edition of the French 3R meeting “Replication, Recombination, Repair” - Last week for abstract submission!

📆 May 18 to 21, 2026
📍 Belambra Club Presqu’île du Ponant in La Grande-Motte, France

🔗 www.2026.3r-meeting.fr/page/home/

Thanks so much for highlighting our study! The tool is versatile and ready to uncover so much new knowledge 😀

I am happy to share our latest publication.

In this work, we show that OGG1 association with DNA during lesion search involves local DNA distortion promoting base unstacking. This process is tightly controlled by conserved residues to ensure stable association without compromising fast scanning.

We are thrilled to share our latest publication in Cell Reports (Cell Press)!

A huge thank you to everyone involved in this work!

📖 Read the full paper here: www.cell.com/cell-reports...

In this study, we reveal that OGG1’s association with DNA during lesion searching involves local DNA distortion, promoting base unstacking. This process is tightly regulated by conserved amino acids encircling the double helix, ensuring stable binding without compromising rapid scanning.

A coordinated haptic mechanism ensures efficient DNA sampling by the 8-oxoguanine glycosylase OGG1 Martinez et al. propose that OGG1 association with DNA during lesion seeking involves local DNA distortion promoting base unstacking. This process is tightly controlled by several conserved amino acids encircling the double helix, ensuring a sufficiently stable association during base interrogation while avoiding excessive engagement detrimental for fast scanning.

A coordinated haptic mechanism ensures efficient DNA sampling by the 8-oxoguanine glycosylase OGG1

✍️ The Cadoret Lab contributed to a new publication

📖 @natcomms.nature.com
📃 DNA methylation and lncRNA control asynchronous DNA replication at specific imprinted gene domains
🔗 www.nature.com/articles/s41...

A genetically encoded device for transcriptome storage in mammalian cells Understanding how cells make decisions over time requires the ability to link past molecular states to future phenotypic outcomes. We present TimeVault, a genetically encoded system that records and s...

🧬🔬@science.org A genetically encoded device for #transcriptome storage in mammalian cells | Science www.science.org/doi/10.1126/... @broadinstitute.org

L'IGDR @igdrennes.bsky.social et la Faculté des Sciences de l'Université de Rennes annoncent l'ouverture d'un poste de Professeur des Universités en section CNU 65 "Régulations génétiques et épigénétiques" avec l'opportunité de rejoindre notre équipe @spartelab.bsky.social #PU #CNU65

The IGDR @igdrennes.bsky.social and the Faculty of Sciences at the University of Rennes announce an opening for a University Professor position in CNU Section 65 "Genetic and Epigenetic Regulations" with the opportunity to join our team @spartelab.bsky.social #PU #CNU65.

The IGDR @igdrennes.bsky.social and the Faculty of Sciences at the University of Rennes announce an opening for a University Professor position in CNU Section 65 "Genetic and Epigenetic Regulations" with the opportunity to join the SP@RTE team @spartelab.bsky.social #PU #CNU65.

L'IGDR @igdrennes.bsky.social et la Faculté des Sciences de l'Université de Rennes annoncent l'ouverture d'un poste de Professeur des Universités en section CNU 65 "Régulations génétiques et épigénétiques" avec l'opportunité de rejoindre l'équipe SP@RTE @spartelab.bsky.social #PU #CNU65

MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony - Nature Methods The micro isolation (MISO) method combines microfluidics-based protein purification with cryo-EM grid preparation, enabling cryo-EM structure determination of soluble and membrane proteins starting fr...

MISO: a micro isolation method for single-particle cryo-EM grid preparation for extremely small sample amounts.

www.nature.com/articles/s41...

Filament formation and NAD processing by noncanonical human FAM118 sirtuins - Nature Structural & Molecular Biology Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that FAM118A/B processing of NAD involves head-to-tail filament...

Please check our collaborative paper with Marcin Suskiewicz and Sebastien Huet labs on FAM118 proteins – non-canonical sirtuins that form filaments and act as NADases in human and other vertebrate cells.
www.nature.com/articles/s41...

Filament formation and NAD processing by noncanonical human FAM118 sirtuins Nature Structural & Molecular Biology - Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that...

Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.

Agreed! This review thoughtfully and comprehensively dives into the world of HPF1. The questions at the end are on point!

Histone PARylation factor 1: a review of its role in the DNA damage response Abstract. Although poly-(ADP ribose) polymerase 1 (PARP1) and PARylation of histones have been known for over 50 years and have been successfully targeted

Beautiful review from J. Rudolph on Histone PARylation factor 1: a review of its role in the DNA damage response url: academic.oup.com/nar/article/...

#JobOffer

💼 Un poste susceptible d’être vacant de Professeur en stabilité des génomes devrait s’ouvrir pour la rentrée 2026 au sein de l’Institut Jacques Monod

➡️ Informations concernant ce poste et la procédure de candidature 🔗 www.ijm.fr/poste-de-pro...

Finally, someone has solved a real problem with AI! No more having to take a paper in the format for a journal that rejected you, and reformat it for a new journal. Well done!! formatmypaper.com

SPT of DNA repair proteins after cell irradiation by Ashli Kobayashi @asaki-kobayashi.bsky.social and Romane Riou. Setup by Abbelight @abbelight.bsky.social and irradiator by Errol Laser @errol-laser.bsky.social. Look at that recruitment!
#Mifobio2025 @gdrimabio.bsky.social

New method paper:
Bennie Lemmens et al @scilifelab.se describe 3D-SPARK, a super-resolution microscopy method that maps changes in DNA replication nanostructures in response to genetic perturbations or drugs, linking spatial cell biology with DNA synthesis dynamics
www.embopress.org/doi/full/10....

CCDC103-mediated assembly of the R2C complex links RUVBL1-RUVBL2 to Primary Ciliary Dyskinesia Primary ciliary dyskinesia (PCD) is a genetic disorder caused by defective cilia motility, powered by axonemal dynein motors. Assembly of these motors is facilitated by the molecular chaperone HSP90, ...

New pre-print on the role of CCDC103/DNAAF19 in dynein assembly highlighting it's link to PCD.

www.biorxiv.org/content/10.1...

Exciting new study from new-PI Mohinder Pal on the role of CCDC103 and it's links to PCD as the most recent dynein assembly factor (DNAAF19). Congratulations to the entire team.

@Mohinder Pal (currently not on Bluesky)
@mike-wieczorek.bsky.social
@laurencepearl.bsky.social

US drugmaker Merck scraps £1bn London research centre and cuts 125 science jobs New blow to UK’s key life science sector as industry body says country is losing ground on investment and research

Academic research generates almost all intellecutal innovation in the life sciences. With big pharma refusing to contribute, maybe it's time to stop giving them our research outputs cheaply, and instead having globally nationalised means of translating innovation?
www.theguardian.com/business/202...

I supervise an excellent postdoc who's looking for a postdoc or associate position in < 1 yr. Highly skilled in chem biol and biochem (peptide synthesis, native chem ligation, bacterial expression, purification, intein-based splicing, crystallography, interaction assays). Contact me if interested.

DNA2 enables growth by restricting recombination-restarted replication - Nature DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...

#New-Paper Rass laboratory reveal DNA2 to be a gatekeeper to homologous recombination at stalled DNA replication forks, rrequired to stave off cellular senescence. Their findings provide a molecular explanation for the association of DNA2 with primordial dwarfism and cancer.

doi.org/10.1038/s415...

The application deadline has been extended for a postdoc position in our lab until August 22. Please enquire/apply if you are interested in ADP-ribosylation, ubiquitination, genome stability or immunity.
my.corehr.com/pls/uoxrecru...

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

My former lab at the University of Oxford is looking for a postdoc to study ADP-ribosylation and ubiquitination signalling in DNA damage using biochemistry and structural biology.

my.corehr.com/pls/uoxrecru...

Informal inquiries can be sent to Prof Ivan Ahel via email: ivan.ahel@path.ox.ac.uk.