Conner Langeberg

"Use it or lose it." This is great advice for exercise, but when cells need to slow down or go dormant, they need to store ribosomes to recover growth in the future. They use hibernation factors to do this. Here is our latest story on how archaea hibernate ribosomes (1/7):
doi.org/10.64898/202...

Excited to share our first work on protein design! Huge thanks to the entire team, and especially to Bel, Evan, to the Doudna, Jacobsen, Cate, Banfield labs, all co-authors, and my D-lab mates! 💫

Viral enzymes degrade to evade Across all domains of life, immune systems rely on nucleotide-based signaling molecules to activate defense responses. In a recent Cell Host & Microbe study, Doherty, Nomburg, and colleagues identify ...

A very nice Preview of our work in
@cp-cellhostmicrobe.bsky.social
this morning from Chrishan Fernando & Nicole D. Marino! www.cell.com/cell-host-mi...

A conserved viral RNA fold enables nuclease resistance across kingdoms of life Abstract. Viral exoribonuclease-resistant RNA (xrRNA) structures block cellular nucleases to produce subgenomic viral RNAs during infection. High sequence

Our study from the Steckelberg lab is out now in @narjournal.bsky.social !! We dove into a new nuclease-blocking viral #RNA structure important for viral infection, which provided evidence of similar structure-based strategies across diverse viral families. academic.oup.com/nar/article/...

Archaeal virus entry and egress Abstract. Archaeal viruses display a high degree of structural and genomic diversity. Few details are known about the mechanisms by which these viruses ent

Archaeal virus entry and egress
Archaea also have viruses and they are cool!
#microbiology #virology #virus #viruses
@femsmicro.org @femsjournals.bsky.social
academic.oup.com/microlife/ar...

Structural insights into transcriptional regulation by the helicase RECQL5 - Nature Structural & Molecular Biology Florez Ariza and Lue et al. use cryo-electron microscopy to investigate how the RECQL5 helicase regulates transcription. Their structural findings suggest that RECQL5 can modulate RNA polymerase II’s ...

Our work, co-led by @alfredo0712.bsky.social and @nlue8.bsky.social is out in @natsmb.nature.com Shout out to coauthors P.Grob, B. Kaeser, J.Fang, and S. Kassube for their contributions and thanks to the reviewers for the suggestions that led to the final work.
www.nature.com/articles/s41...

We are happy to share that our snR30 story is finally out in @natureportfolio.nature.com 🥳 We report the first structure of a H/ACA snoRNP acting in ribosome synthesis thereby providing a detailed structural and biochemical view of the snR30 snoRNP guiding local 18S rRNA subdomain folding. 👇👇👇

Tick-borne flavivirus exoribonuclease-resistant RNAs contain a double loop structure - Nature Communications Many flaviviruses generate specialized non-coding RNAs that resist degradation by host exonucleases, promoting viral infection. Here, the authors characterize an RNA element in Powassan virus, highlig...

Final grad paper is officially out! Grateful for the incredible team and all the support along the way!
www.nature.com/articles/s41...

A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway Microbial and viral co-evolution has created immunity mechanisms involving oligonucleotide signaling that share mechanistic features with human anti-viral systems. In these pathways, including CBASS a...

Preprint alert! ✨ In this project that I co-led with @benadler.bsky.social, we show that a miniature CRISPR-Cas10-like enzyme, mCpol, uses a novel inverse signaling mechanism to prevent the spread of viruses that attempt immune evasion by depleting host cyclic nucleotides.

Check it out:

Giving a seminar for the Genome Engineering Seminar Series at Harvard this Monday, April 7 at 1pm EST / 10am PST! 🎙️ I’ll be talking about our Cas9 work—if you’ve ever wondered what the recipe is for high-efficiency CRISPR genome editing, come hang out!🧬💥
📍 Zoom link: harvard.zoom.us/j/94394339529

Really nice issue of RNA this month featuring many Perspective pieces on RNA in disease. rnajournal.cshlp.org/content/31/3...

We present Rfam-based RNA structural alignments and other structure-based inputs for RNA 3D structure prediction by deep learning.
www.biorxiv.org/content/10.1...

Single-molecule live-cell RNA imaging with CRISPR–Csm - Nature Biotechnology Single-molecule live-cell fluorescence in situ hybridization uses an RNA-targeting CRISPR–Csm complex to image and track endogenous RNAs.

Single-molecule live-cell RNA imaging with CRISPR–Csm - @doudna-lab.bsky.social @qb3-berkeley.bsky.social @innovativegenomics.bsky.social go.nature.com/3EJPKsM

Latent Labs comes out of stealth today with $50M funding. Our goal? To push the frontiers of generative biology, giving partners instant access to tools capable of accelerating drug design

I had the pleasure of co-hosting this new career exploration podcast from ASBMB- tune in to hear from my fellow committee member Tom Kiselak about being a patent attorney!

Excited to see this work finally out, it was a pleasure to be involved!

Third-generation cephalosporin antibiotics induce phage bursts in the human gut microbiome. The use of antibiotics disrupts the gut microbiota, potentially leading to long-term health issues and the spread of resistance. To investigate the impact of antibiotics on phage populations, we follo...

Excited🚨to share our first work on the human gut phageome doi.org/10.1101/2025...
Always inspiring to work with @epcrocha.bsky.social , Erick D, Camille d'H and everyone else within this super dynamic consortium
@inrae-france.bsky.social+@pasteur.fr+@inserm.fr! Many thanks for this great journey!🙌

Today, the IGI turns 10! We’ve grown from a fledgling initiative focused around developing #CRISPR tools to a full-blown research institute bringing together leaders in basic & translational research! ⚕️💊🌱☀️

Take a look at our timeline & key achievements from our first decade: ow.ly/GzVt50UTsbP

A conserved class of viral RNA structures regulates translation reinitiation through dynamic ribosome interactions pubmed.ncbi.nlm.nih.gov/39893634/ #cryoEM

Thrilled to have been involved in this work and looking forward to what the Sherlock Lab has in store!

Thioesters Support Efficient Protein Biosynthesis by the Ribosome Thioesters are critical chemical intermediates in numerous extant biochemical reactions and are invoked as key reagents during prebiotic peptide synthesis on an evolving Earth. Here we asked if a thio...

✨ New from C-GEM in ACS Central Science:
Thioesters Support Efficient Protein Biosynthesis by the Ribosome
pubs.acs.org/doi/10.1021/...

Congrats to Alexandra & Jacob, along with Isaac, Jess, @alexsolivan.bsky.social, @nxhamlish.bsky.social, Katie, Alanna, Jamie, and Scott!
#NSFfunded @pubs.acs.org

Long-term imaging of individual ribosomes reveals ribosome cooperativity in mRNA translation Ribosomes cooperate through transient collisions to ensure efficient translation.

www.cell.com/cell/fulltex...

Out today @ Science: TIR signaling activates caspase-like immunity in bacteria

We report a new immune signaling molecule: N7-cADPR. Produced by phage-induced TIRs and activates a defensive bacterial caspase

Congrats Francois Rousset, Ilya Osterman and coauthors!

www.science.org/doi/10.1126/...

Our paper out @Nature: CARD domains mediate anti-phage defense in bacterial gasdermin systems

CARDs are essential for caspase recruitment during human inflammasome activation. We now find them in bacterial immune systems

Congrats Tana Wein! Thank you Kranzusch lab!
www.nature.com/articles/s41...

C-terminal amides mark proteins for degradation via SCF–FBXO31 - Nature SCF–FBXO31 scans proteins for C-terminal amidation and marks them for subsequent proteasomal degradation.

scary but fascinating - cells have a degradation pathway that hunts down C-terminal scars (amides!) on damaged proteins. just wow. chemical biology plus CRISPR at its best. big congrats to all authors!

www.nature.com/articles/s41...

In addition to today's posted work by @cryoclem.bsky.social on Archaeal ribosomes we further expanded RAPPL (www.biorxiv.org/content/10.1...) on multiple other complexes that work with ribosomes, subunits or even off the ribosome. Soon more...

CARD domains mediate anti-phage defence in bacterial gasdermin systems - Nature Caspase recruitment domains (CARDs) are present in defence systems that protect bacteria against phage, where the bacterial CARD domain is essential for protease-mediated activation of bacterial gasdermins that promote cell death.

Nature research paper: CARD domains mediate anti-phage defence in bacterial gasdermin systems

https://go.nature.com/3CvoD3W

Integrons are anti-phage defence libraries in Vibrio parahaemolyticus Nature Microbiology - Integrons are genetic elements that capture and store gene cassettes in Vibrio species. Bioinformatic and molecular techniques show that these regions can be hotspots of phage...

It’s out! Brand new work from my PostDoc in @themaxwelllab.bsky.social exploring anti-phage defence in V. parahaemolyticus: Integrons are anti-phage defence libraries in Vibrio parahaemolyticus
rdcu.be/d7EsN

GitHub - bjing2016/alphaflow: AlphaFold Meets Flow Matching for Generating Protein Ensembles AlphaFold Meets Flow Matching for Generating Protein Ensembles - bjing2016/alphaflow

New Title Alert: AlphaFlow- a modified version of AlphaFold, fine-tuned with a flow matching objective, designed for generative modeling of protein conformational ensembles.

Learn more here: https://buff.ly/4gwVW5o

#SBGrid #SBGridsoftware #StructuralBiology

Kiwa is a bacterial membrane-embedded defence supercomplex activated by phage-induced membrane changes Bacteria and archaea deploy diverse, sophisticated defence systems to counter virus infection, yet many immunity mechanisms remain poorly understood. Here, we characterise the Kiwa defence system as a...

Happy to share our latest findings on Kiwa. Foundational work from Thomas, with Zhiying and Yi providing beautiful structures and molecular insights. Ever grateful for the collaborations with Eugene and Dinshaw. More to come 🙃 doi.org/10.1101/2023...