Korak Ray

Visual advertisement providing details for the 2026 Single Molecule Approaches to Biology GRS in Les Diablerets, Switzerland in June 27-28, 2026. Two-day event for early-career single-molecule biophysicists, with a keynote session, 12 selected talks, 2 poster sessions, and mentorship component. For more information contact Alba or Quentin or see the official GRS website.

🔬 Calling all early-career researchers (ECRs) for the GRS Single Molecule Approaches to Biology!

🌎 Location: Les Diablerets, Switzerland

📆 Save the date: June 27-28, 2026

🔗 More information: www.grc.org/single-molec...

#biology #biophysics #single-molecule #microscopy #ECR

Energy-landscape model of RF2 activation. Comparison of termination complexes (TC), wild-type ArfA-bound nonstop elongation complexes (nsECs), and loss-of-function ArfA mutant-bound nsECs reveals that wild-type ArfA promotes RF2 docking to nsECs by reshaping the conformational cascade.

Slightly overdue article highlight!

In PNAS, we use smFRET to reveal how polypeptide release factor 2 (RF2) undergoes a cascade of structural changes to catalyze polypeptide release during translation termination and ArfA-mediated ribosome rescue.

🧵⬇️
doi.org/10.1073/pnas...

A bit of good news!

Part of my PhD work in collaboration with @ssjang.bsky.social and David Lynall is out in @narjournal.bsky.social! We uncover a surprising mechanism by which an ultrastable RNA stem-loop tunes its flexibility, enabling it to both fold rapidly and resist unfolding.

Congratulations, John! Great to see this out!

Congratulations, John!

Are you at #bps2025 and want to hear about:

HSV-1 DNA replication,
DNA looping,
#smFRET,
TIRF,
optical tweezers
or cryoEM?

Come and talk to me at my poster B175 on Wed, 10.30am!

Precise intracellular uptake and endosomal release of diverse functional mRNA payloads via glutathione-responsive nanogels We present a novel, highly customizable glutathione-responsive nanogel (NG) platform for efficient mRNA delivery with precise mRNA payload release con…

Exciting new research from the Cellular Stress and Single Molecule Imaging groups has created a new technique for transfecting cells that could pave the way for new gene therapies!

We'll be recapping some of the highlights from the article in this thread.

🧵1/6

GonzalezBiophysicsLab tmaven · Discussions Explore the GitHub Discussions forum for GonzalezBiophysicsLab tmaven. Discuss code, ask questions & collaborate with the developer community.

Also make sure you have the latest version of the software for your analysis needs. The software is always being updated. You can find the latest updates at the website gonzalezbiophysicslab.github.io/tmaven/
Also, please follow and take part in the discussions at github.com/GonzalezBiop...

Increasing the accuracy of single-molecule data analysis using tMAVEN Time-dependent single-molecule experiments contain rich kinetic information about the functional dynamics of biomolecules. A key step in extracting th…

tMAVEN, our open-source comprehensive platform for single-molecule data analysis, is finally here! Check out our article describing the platform and how we use it to investigate the use of kinetic models to extract mechanistic info from data, out in Biophys J. www.sciencedirect.com/science/arti...

Spooky season reminder that, at its core, The Monster Mash is a cautionary tale about working in the lab late one night and, ultimately, the dangers of poor work/life balance under late capitalism.

That sounds really interesting and definitely something to try out. Thank you for the suggestion!

The meta-analysis is complicated because
a) different software were historically used with other equipment that confound the software's effect.
b) metadata on software in PDB files had inconsistent reporting.

So, our analysis is purely descriptive. With those caveats in mind however, yes, there is

GitHub - bayes-shape-calc/HARP: Hierarchical Atomic Resolution Perception Hierarchical Atomic Resolution Perception. Contribute to bayes-shape-calc/HARP development by creating an account on GitHub.

(6/6) Our method, which we call HARP, is open-source and freely available as a downloadable application which you can use to test the local resolution and map-to-model agreement of your favourite (or your own!) cryoEM structure! Give it a try! github.com/bayes-shape-...

(5/6) We also see how sensitive this framework is to local chemical changes, with specific amino acids that are known to appear distorted in cryoEM maps (Glu, Asp, Cys), showing lower levels of atomic information than similarly sized residues.

(4/6)...revealing, e.g., how the cryoEM resolution revolution has lead to increased atomic resolution over the years, and, surprisingly, a seasonal trend in atomic resolution (based on when structures are deposited in the PDB) that seems to correlate with the academic calendar.

(3/6) Comparison between the atomic and residue models in particular reveals the extent of local atomic resolution at individual residues. As expected, on average, this local atomic resolution measure correlates with the standard FSC resolution of maps, but yields richer info...

(2/6) We utilise the hierarchical nature of biomolecular structure to define models of cryoEM density at different structural levels, which can be used to select the level that agrees best with experimental data, revealing the extent of structural information present in the data.

Scale-dependent hierarchical resolution: applications to atomic resolution and model validation in c... bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

🚨 Preprint news!! 🚨 The collaboration with @ckinzthompson on a novel definition of resolution and how it can be used to calculate the local atomic resolution and map-to-model correspondence in cryoEM structures is finally on bioRxiv!!!! www.biorxiv.org/content/10.1... 🧵(1/6)

GitHub - bayes-shape-calc/HARP: Hierarchical Atomic Resolution Perception Hierarchical Atomic Resolution Perception. Contribute to bayes-shape-calc/HARP development by creating an account on GitHub.

(6/6) Our method, which we call HARP, is open-source and freely available as a downloadable application which you can use to test the local resolution and map-to-model agreement of your favourite (or your own!) cryoEM structure! Give it a try! github.com/bayes-shape-...

(5/6) We also see how sensitive this framework is to local chemical changes, with specific amino acids that are known to appear distorted in cryoEM maps (Glu, Asp, Cys), showing lower levels of atomic information than similarly sized residues.

(4/6)...revealing, e.g., how the cryoEM resolution revolution has lead to increased atomic resolution over the years, and, surprisingly, a seasonal trend in atomic resolution (based on when structures are deposited in the PDB) that seems to correlate with the academic calendar.

(3/6) Comparison between the atomic and residue models in particular reveals the extent of local atomic resolution at individual residues. As expected, on average, this local atomic resolution measure correlates with the standard FSC resolution of maps, but yields richer info...

(2/6) We utilise the hierarchical nature of biomolecular structure to define models of cryoEM density at different structural levels, which can be used to select the level that agrees best with experimental data, revealing the extent of structural information present in the data.

BREAKING NEWS: Three scientists have won the 2023 #NobelPrize in Chemistry for their research on the discovery and synthesis of quantum dots. Moungi G. Bawendi, Louis E. Brus and Alexei I. Ekimov share prize for research that added color to nanotechnology: cen.acs.org/people/nobel...