Mohamed ElTanbouly

Congrats Ruth and colleagues!!!

I enjoyed reading this...Fascinating discovery

Really great work

Congrats on your terrific effort Payal and co!!! 🎉

Very nice and comprehensive study.

All the best with what comes next.

Novel multicellular prokaryote discovered next to an underground stream A new bacterium isolated from an underground stream shows a novel multicellularity by self-organizing its filamentous cells like a liquid crystal and accommodating daughter cells in a sequential manne...

What are your thoughts on this?

elifesciences.org/articles/71920

Thanks very much for advising.

Might I ask you if any of these talks are available on NIHvideocast or youtube? Or accessible online?

I tried to access but it says that it's only available to Harvard folks

What am I missing? Aside from a Harvard ID card

Tissue-resident memory CD8 T cell diversity is spatiotemporally imprinted - Nature Profiling of the location and transcriptome of tissue-resident memory CD8 T cell formation at single-transcript resolution finds regionalized signalling as the basis of immune diversity in t...

On an irrelevant note, congratulations on this terrific paper
www.nature.com/articles/s41...

This is fascinating!

These are all immune (lymphocyte-relevant) genes!!!!!

Mitochondria is the powerhouse of the cell

Chloroplast is the cell's farm

ER is the factory and ribosomes are the workers

Plasma membrane are the city walls

Cilia and flagella are the stirring wheels and propellers

Exosomes and endosomes are the email/post office

Many thanks for all these terrific insights.

Tcf7 activates genes with CTNNB1 (β-catenin)?

What signals are upstream of this? Or are they constitutively active?

Congrats @danmcmanus123.bsky.social

Very nice work!

Unrelated request: Would be nice if someone figured out what maintains Tcf7 expression and activity in CD8 stemlike (± mem T cells). It was argued in a recent conference that it's Wnt signaling but I can't see how in the absence of Fzd expression in T cells.

Clones usually encompass cell members with heterogenous cell states, too.

Makes sense. Thanks for clarifying.

Selective expression of the interleukin 7 receptor identifies effector CD8 T cells that give rise to long-lived memory cells - Nature Immunology Nature Immunology - Selective expression of the interleukin 7 receptor identifies effector CD8 T cells that give rise to long-lived memory cells

I think they need Il7r re-expression (and Il2r downregulation)

www.nature.com/articles/ni1...

www.sciencedirect.com/science/arti...

Replicative history marks transcriptional and functional disparity in the CD8+ T cell memory pool - Nature Immunology Schumacher and colleagues have designed a reporter system that allows in vivo tracking of replicative history over many cell generations. Using this system to study acute T cell responses, they uncove...

1-This paper?
www.nature.com/articles/s41...
Might you elaborate on where they discussed this (PD-1) here?

I recall they assessed "renewal potential" (Tcm-ness based on prior division no. and quiescence state)

2-Looking forward to reading your group's findings. Sounds very interesting.

They're memory precursors.

When antigen goes away, most clones contract and these cells make up the central memory (Tcm).

In chronic infection, they act as transient amplifiers differentiating into terminal eff and new precursors.

Statement from PMID: 39708817

Introduction to PMID: 34822279

1-Incredible figure presentation and clarity

2-Eight years of work

3-Favorite statement (reminded me of another statement in an equally authoritative paper). Both are presented in screenshots below.

4-Builds upon earlier work by Peng et al from Jameson group
www.sciencedirect.com/science/arti...

Thank you :) for the very kind feedback.

I'm still enjoying it so will keep summarizing/reviewing

All the best with your work + life

Many thanks

Could I ask your take on the data showing "Treg conversion to Tfh"?

What (signals) persuades them?

PMID: 39662506
PMID: 19286559

Unsolicited feedback: This sounds so cool.

Can't wait to read your findings. All the best with this (and everything, but primarily this).

Thank you for the delightful feedback. Much appreciated!

Thank you for providing the source.

And for sharing these fascinating details about Brenner

Might you share the PMID or book/article title?

For some reason, I tried looking this up and couldn't find it.

Many thanks either way

Looking forward!!!

Congratulations!

Best wishes with equally wonderful papers and discoveries

Not useless...Technologies are ephemeral but ideas and insights are not :)

I was reading this Cell issue celebrating Molecular Biology and this comment is along the lines you described. Amazing how long it took to sequence a bit of DNA.

www.cell.com/cell/fulltex...

I remember in a break between sessions of an online conference (2021), two well-known PIs talked about how they used to move tubes between different temperature water baths before thermal cyclers were invented.

I ran a couple of Microarrays in my distant youth and now feel these were dark ages.