IgnatovaLab

Context-dependent translation inhibition as a cancer therapeutic modality - Nature Communications Robust protein synthesis by the ribosome is required for rapid cancer growth. Here authors present interdictors, small molecule inhibitors of protein synthesis with context-dependent activity that inh...

Incredibly proud of our small but mighty team at Interdict Bio as we bring the interdictor modality closer to the clinic! Check out our first publication that lays the foundation for context-dependent translation modulators as a therapeutic approach for cancer!
www.nature.com/articles/s41...

Single-cell and isoform-specific translational profiling of the mouse brain - Nature Post-transcriptional regulation of mRNA translation was explored using Ribo-STAMP and single-cell RNA sequencing to reveal cell-type-specific and isoform-specific translation patterns across hippocampal neuronal and non-neuronal cell types, highlighting functional differences between CA1 and CA3.

Nature research paper: Single-cell and isoform-specific translational profiling of the mouse brain

go.nature.com/4aYPQu7

Recruitment of bifunctional regulator thermospermine to methylated ribosomes directs xylem fate Polyamines are often associated with ribosomes and are thought to stabilize their integrity. In Arabidopsis, the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SU...

In @science.org this week, thermospermine affects ribosome methylation, which in turn regulates translation of xylem development proteins www.science.org/doi/10.1126/...

#plantscience

Blocking RAN translation without altering repeat RNAs rescues C9ORF72-related ALS and FTD phenotypes GGGGCC (G4C2) repeat expansion in C9ORF72 is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Toxicity is thought to result from the accumulation...

I'm thrilled to anounce that our latest study on ALS/FTD neurodegenerative diseases has been published. Very proud to be part of this excting project. Many thanks to Clotilde Lagier Tourenne for an amazing longlasting and fruitfull collaboration, #ALS #FTD #Ribosomes
www.science.org/doi/10.1126/...

Structures of naked mole-rat, tuco-tuco, and guinea pig ribosomes—is rRNA fragmentation linked to translational fidelity? Abstract. Ribosomes are central to protein synthesis in all organisms. In mammals, the ribosome functional core is highly conserved. Remarkably, two rodent

now online:
academic.oup.com/nar/article/...

Our paper on the structural and functional basis of the human dimeric OS9–SEL1L–HRD1 ERAD complex, together with @qilabuva.bsky.social, is now out! Huge congratulations to Leo, Emir, and Elina!
www.nature.com/articles/s41...

Congrats Ahmad and team!

ADAM-tRNA-seq: an optimized approach for demultiplexing and enhanced hierarchal mapping in direct tRNA sequencing Abstract. Transfer RNAs (tRNAs) play an essential role in protein synthesis and cellular homeostasis, and their dysregulation is associated with various hu

Interested in direct tRNA-seq by Nanopore? We developed a new RNA-based barcoding aproach for seq parallelization; it is also cost-saving. Scripts available on github. #tRNA #Nanopore

track.smtpsendmail.com/9032119/c?p=...

Congratulation Agnes! Truly deserved!

What a beatiful study: GCN2 is activated by ribosome collisions

Congrats Susan! Beatiful story!

Translation landscape of stress granules Cryo–electron tomography visualizes stress granules in situ, revealing their spatial interplay with translation machinery.

Translation landscape of stress granules | Science Advances

www.science.org/doi/10.1126/...

Thrilled to announce our #preprint on a new factor in the last line of defense in #translation quality control out on #bioRXiv! Spearheaded by fantastic PhD student @kaushikiyer.bsky.social, supported by Chloé Walter, Alina Kraft, Max Müller and Lena Tittel.

Deep generative models design mRNA sequences with enhanced translational capacity and stability Despite the success of mRNA COVID-19 vaccines, extending this modality to more diseases necessitates substantial enhancements. We present GEMORNA, a generative RNA model that utilizes Transformer arch...

Deep generative models design mRNA sequences with enhanced translational capacity and stability. #Science

www.science.org/doi/10.1126/...

New Banksy artwork appears on court building showing judge attacking protester Banksy's mural shows a protester on the ground holding a blood-spattered placard, while the judge, in a wig and gown, looms over them wielding a gavel.

New Banksy artwork appears on court building showing judge attacking protester

Suppressor tRNAs as personalized therapy for nonsense mutation-associated pathologies Nonsense mutations – associated with many devastating genetic disorders that currently lack effective treatments – prematurely terminate protein synth…

A new review on suppressor-tRNA therapies, we wrote with Suki Albers 👇🏻
www.sciencedirect.com/science/arti...

Here, we show that bacterial ribosomes—often viewed as conserved drug targets—actually vary across species due to extensive sequence variation in their drug-binding residues. In some cases, this divergence may lead to intrinsic drug resistance in certain bacterial clades: doi.org/10.1016/j.ce...

Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org‬: www.embopress.org/doi/full/10....

I am excited to share the second half of my postdoctoral work in the Yeo lab at UCSD! We found that neuronal aging leads to the accumulation of mitochondrial double-stranded RNA, which triggers the chronic stress response. 1/ www.biorxiv.org/content/10.1...

A rapid, simple, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies Nature Communications - Here, the authors present a method to rapidly isolate actively translating ribosomes in a time- and cost-effective manner using poly-lysine. The method is compatible with a...

Our RAPPL purification of ribosomes and associated material is out. I am really proud of this study in collaboration primary with Pavlovic-Djuranovic lab, as well as Jovanovic and Hashem Labs. This method aims to replace 60 years old purification of ribosomes by sucrose cushions

rdcu.be/ezlhg

Deutschlandkarte der Verteilung der Exzellenzcluster auf Bundesländer und Universitäten.

Die #Exzellenzcluster stehen fest: Heute hat die Exzellenzkommission 70 Projekte zur Förderung ausgewählt. 45 Cluster werden fortgesetzt, 25 neu eingerichtet. Die Förderung beginnt ab 1. Jan. 2026 für 7 Jahre, die Fördersumme beträgt insg. 539 Mio. €/Jahr. Die Liste: www.dfg.de/resource/blo... 1/3

m6A modification is incorporated into bacterial mRNA without specific functional benefit Abstract. N6-Methyladenosine (m6A), the most abundant modification in eukaryotic messenger RNAs (mRNAs), has also been found at a low level in bacterial mR

Delighted to share our newest publication - beautiful collaboration with Ya-Ming Hou’s lab. If you wonder whether bacterial mRNAs contain m6A (yes they do) and whether it matters (does not seem to) look here 👇🏻

academic.oup.com/nar/article/...

Scientific Editor (Absence cover) – EMBO About us EMBO is a not-for-profit organization dedicated to promoting excellence in the life sciences in Europe and beyond. We fund talented researchers at all career stages, facilitate scientific…

EMBO is #hiring a Scientific Editor (Absence cover) to join @emboreports.org. Apply by 8 June: buff.ly/seVUgYW 🧪

Mammalian cells eliminate nascent chains stalled during co-translational protein translocation by activating a ribosome UFMylation-dependent TAQC process at the ER. Ennis, Wang, Ye et al. establish a role for NEMF-mediated CAT tailing in TAQC. rupress.org/jcb/article/...
#ER_literature

Age-related blood condition counteracted with a common diabetes drug Metformin could reduce people’s risk of certain age-associated blood cancers, but more work is needed to identify who is likely to benefit.

www.nature.com/articles/d41...

Turn cold tumors hot by reprogramming the tumor microenvironment - Nature Biotechnology The identification of novel targets together with technological advances is transforming our ability to effectively modulate the tumor microenvironment.

www.nature.com/articles/s41...

Cells are swapping their mitochondria. What does this mean for our health? Researchers are studying why the energy factories are moving between cells and whether the process can be harnessed to treat cancer and other diseases.

Cells are swapping mitochondria. Wondering whether they also swap much smaller cellular entities, e.g. ribosomes?
www.nature.com/articles/d41...

A conserved opal termination codon optimizes a temperature-dependent trade-off between protein production and processing in alphaviruses Alphaviruses optimize viral polymerase production and polyprotein processing at distinct temperatures via a premature stop codon.

www.science.org/doi/10.1126/...

Blood–brain-barrier-crossing lipid nanoparticles for mRNA delivery to the central nervous system - Nature Materials A lipid-nanoparticle-based formulation incorporating engineered lipids containing a blood–brain-barrier-crossing moiety effectively delivers mRNA to neurons and astrocytes following systemic administr...

www.nature.com/articles/s41...

A natural experiment on the effect of herpes zoster vaccination on dementia - Nature Using a natural experiment that avoids common bias concerns, this study finds that the live-attenuated shingles vaccine reduced the probability of a new dementia diagnosis within a follow-up period of...

Very compelling study showing the association between herpes zoster vaccination and dementia prevention. The authors leveraged a unique cohort for this natural experiment which shows a reduction in dementia risk of 20% in vaccinated individuals. #MedSky #IDSky 🧪
www.nature.com/articles/s41...