Fernando Gomez-Herreros Lab

A comprehensive genetic catalog of human double-strand break repair The analysis of DNA sequence outcomes provides molecular insights into double-strand break (DSB) repair mechanisms. Using parallel in-pool profiling of Cas9-induced insertions and deletions (indels) w...

Kickstarting our Bluesky🦋 account with great news!
Our REPAIRome study finally out today in
@science.org
www.science.org/doi/10.1126/...

DNA2 enables growth by restricting recombination-restarted replication - Nature DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...

#New-Paper Rass laboratory reveal DNA2 to be a gatekeeper to homologous recombination at stalled DNA replication forks, rrequired to stave off cellular senescence. Their findings provide a molecular explanation for the association of DNA2 with primordial dwarfism and cancer.

doi.org/10.1038/s415...

👉EWS::FLI1 modulates R-loop metabolism via DHX9, driving increased vulnerability to TOP1-targeting agents.
👉Targeting TOP1 in EWS cells destabilizes the genome and provokes strong replication stress and cell death in EwS cells and tumors

Grateful to all collaborators who made this work possible!

EWS::FLI1-DHX9 interaction promotes Ewing sarcoma sensitivity to DNA topoisomerase 1 poisons by altering R-loop metabolism - Oncogene Oncogene - EWS::FLI1-DHX9 interaction promotes Ewing sarcoma sensitivity to DNA topoisomerase 1 poisons by altering R-loop metabolism

Our latest study is now online! A great collaboration between the @fgh-lab.bsky.social and @enriquedealava.bsky.social Labs, driven by the outstanding work of J. Olmedo-Pelayo at @ibis-investigacion.bsky.social Check it out!

PLAMseq enables the proteo-genomic characterization of chromatin-associated proteins and protein interactions in a single experimental workflow. Chromatin Immunoprecipitation (ChIP) and Co-Immunoprecipitation (CoIP) assays are the most common approaches to characterize the genomic localization and protein interactors, respectively, for a prote...

New preprint from the Lab at @cabimer.bsky.social. We present PLAMseq, using TurboID to do like ChIPseq and proximity proteomics in the same protocol with extra powers like mapping protein interactions in the genome.

www.biorxiv.org/content/10.1...