Alexandre Dumoulin

Happy #FluorescenceFriday!

Temporal projection of a time-lapse recording decided to become abstract art. Pretty splashy, isn't it?

PalmBow-labeled commissural axons in culture.

#neuroscience #microscopy #livecellimaging 🧪

A gentle palette of plasma membrane dyes | PNAS Plasma membrane (PM) stains are important organelle markers for monitoring membrane morphology and dynamics. The state-of-the-art PM stains are bri...

Hello,

Yes, this video has been published as a cropped video in this paper: www.pnas.org/doi/10.1073/...
also feel free to use this uncropped video for your lecture!
Cheers

Zurich is ready! 🤘 looking forward to hosting you at our Fish and Chicks seminar!

On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky

Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪

very nice interview Joaquín!

Check it out at PNAS today. Thank you Alexandre for the collaboration!!

PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!

Available @spirochrome.com

Thanks Luc! and yes it is! 😉

Here’s an embryonic axonal growth cone labeled with PK Mem dye (red) and tubulin (blue), imaged every 2 seconds. 🤩

Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social !
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001

Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster Nature Neuroscience - The developmental origin of behavioral individuality is unclear. The authors show that a temporal sequence of genetically encoded stochastic mechanisms explains variation in...

Hot off the press:
The excellent Maheva Andriatsilavo and colleagues show how a temporal sequence of stochastic molecular mechanisms allow the emergence of stereotyped individualised neuronal circuits. Just published in Nature Neuroscience:
rdcu.be/ek010

Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster Nature Neuroscience - The developmental origin of behavioral individuality is unclear. The authors show that a temporal sequence of genetically encoded stochastic mechanisms explains variation in...

New from the lab: @maheandria.bsky.social and colleagues in collaboration with the Hiesinger, Stoeckli and von Kleist labs dissect the sequential emergence of axonal targeting during development in today's issue of Nature Neuroscience:
rdcu.be/ek010

cool!
here are chick commissural axons crossing the neural tube floor plate :): bsky.app/profile/axon...

The SynCAM synaptic cell adhesion molecules are involved in sensory axon pathfinding by regulating axon–axon contacts ABSTRACT. Synaptic cell adhesion molecules (SynCAMs) are crucial for synapse formation and plasticity. However, we have previously demonstrated that SynCAMs are also required during earlier stages of ...

Hi Erica,
thanks!
for 2D cultures: journals.biologists.com/jcs/article/...
for 3D cultures: www.frontiersin.org/journals/mol...
for live imaging I used 8-well IBIDI μ-slides with same PLL/Laminin coating and the same medium as in the JCS paper.
Cheers
Alex

These ones are growing in a dish, but in a developing embryo or brain, each growth cone would be sniffing its way through a complex molecular terrain, often over long distances to find its correct synaptic partners.

Hi Luc, thanks!
it is indeed Spy555-FastAct.
Cheers

it is a filopodial (thin protusions) party in there! 🕺🧐
🧪 #liveimaging

This time-lapse captures 17 hours of axonal growth from a chicken dorsal root ganglion explant, visualized through the actin cytoskeleton using live confocal imaging.
I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉
🧪

Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!

me too! They seem to be retraction fibers that are then fragmenting into these small vesicles. It has been described in some migrating cells/cancer cells before. We are now inestigating it in the context of axon growth and guidance!

Yes, it is, I wrote it in the ALT text but somehow it I can't see it. SPY555-FastAct

And sometimes accross the floor plate 🙂

🤩

The Company of Biologists: celebrating 100 years Summary: As we begin celebrating our 100-year anniversary, we reflect on our extraordinary story so far, consider the Company's current support for our communities and prepare for some inspiring activ...

A brief history of The Company of Biologists - 100 years of supporting scientists!👍👏
journals.biologists.com/jcs/article/...

Join us in beautiful Quintay to celebrate 25 years of the Practical Course on Developmental Biology!
To register go to biodesarrollo.unab.cl/mini-symposi...
Come to enjoy a unique blend of science, sea, and food – all for free!

Addgene: The First Twenty Years Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.

Did you know 2024 was our 20th anniversary? We're closing it out by sharing a brief history of Addgene, with commentary from our incredible community.
Read it below!

blog.addgene.org/add...

Big blue neurons surrounded by small yellow microglia everywhere!

2024 #FluorescenceFriday retrospective: It was a great year in science for microglia, the brain's immune cells! The brain is much more than neurons; these little cells are essential, particularly in inflammatory processes. Check them interacting below! Markers: neurons (🔵 MAP2), microglia (🟡 IBA1)

How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling.
www.biorxiv.org/content/10.1...

Special thanks to @aliceyting.bsky.social for discussions

Fascinating! Congrats!

I am super excited to share our article addressing a longstanding question in neurobiology: how Ca²⁺ signaling cascades initiated at dendritic spines operate at a distance. Check it out here: authors.elsevier.com/sd/article/S... @cp-cell.bsky.social.