@janeliagenie.bsky.social
The Genetically-ENcoded Indicator and Effector (GENIE) project at the Janelia Research Campus of HHMI has the organization, multidisciplinary know-how, and technology to greatly accelerate optimization of protein sensors for observing biological function.
Activation of RubyACRs in the pIP10 neuron suppressed pulse song of male flies during courtship.
🧠🪰 New from GENIE @HHMIJanelia: far-red #RubyACRs from the Spudich Lab enable robust optogenetic inhibition in freely behaving #Drosophila — validated with ephys, imaging & behavior.
📄 Paper bit.ly/3H7S7af
🪰 Get flies bit.ly/41iVNwK
med.uth.edu/bmb/spudich/
#OptoInhibition #FlyNeuro #FlyOptogenetics
Fluorescence images of primary neurons expressing iGABASnFR variants at peak brightness after field stimulation with 40 APs
Improved genetically-encoded GABA indicator are now published. iGABASnFR2 has 4.2X better sensitivity and 20% faster kinetics; detects direction selective GABA release in the retina
📃Preprint: bit.ly/4hISy7b
🧪Plasmids & AAVs: bit.ly/3FDh8Jl @addgene.bsky.social @hhmijanelia.bsky.social #Neuroscience
